Microbial preparation based black and odorous water in-situ remediation method and microbial preparation

A microbial preparation, in-situ remediation technology, applied in biological water/sewage treatment, biological sludge treatment, chemical instruments and methods, etc., can solve the problems of high price, difficult to promote, and biological safety hazards.

Active Publication Date: 2019-02-01
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bioremediation technology is widely used in the treatment of black and odorous rivers. However, the core part of biotechnology is microbial preparations, most of which come from abroad. The price is high and it is difficult to promote. It is also a potential hazard to my country's biosafety.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: In-situ repair of black and odorous water body

[0043] Taking the black and odorous water body of a polluted river in Shenzhen as the restoration object, the main indicators are: the dissolved oxygen (DO) of the overlying water body is 1.8mg / L, the redox potential (ORP) of the sediment is -240mv, and the moisture content of the sediment is 43.7%, the organic matter (TOC) content of the bottom mud is 2.88%, and the total microbial activity of the bottom mud as determined by the FDA method is 0.59A / g.

[0044] The black and odorous water in-situ repair method adopted in this embodiment, the specific steps are as follows:

[0045] S1. Sewage interception: Introduce the running water near the implementation section to the downstream of the implementation section, and prohibit external waste water and domestic garbage from entering the treatment waterway section during the implementation period. Along the width of the waterway, use fishing nets and PE nets to...

Embodiment

[0058] Evaluation of Example Method Application

[0059] In this example, a combined bioremediation method based on microbial preparations is used to build ecological revetments through dredging, aeration and re-oxygenation, adding high-efficiency microbial preparations, and suspended fillers. In-situ restoration, using the self-purification ability of aquatic ecological animals, plants and microorganisms to absorb organic pollutants in the water body, so as to achieve the purpose of water purification.

[0060] ① Dredging to build ecological revetment

[0061] The key to waterway improvement is sewage interception, without which it is impossible to fundamentally improve water quality. Pollution sources of waterways include internal pollution and external pollution: internal pollution generally comes from sediment; external pollution includes domestic sewage, industrial wastewater, rainwater discharge, domestic garbage, dumping of residual mud, etc. The bottom sludge has a h...

Embodiment 2

[0081] Embodiment 2: the preparation of microbial preparation

[0082] The present invention employs microorganisms cultured by standard submerged fermentation methods known in the art.

[0083] ① Bacillus species

[0084] Starter cultures of each of B. subtilis, B. amyloliquefaciens, B. orientalis, and B. natto were grown according to the following general protocol: 18 g of nutrient broth, 18 g of yeast extract, and 36 g of maltodextrin were added to a 1 L Erlenmeyer flask. Add 900ml of distilled deionized water and stir the flask until all dry ingredients are dissolved. The flask was capped and placed in an autoclave operating at 121°C and 15 psi for 30 minutes. After cooling, 100 ml of microbial culture medium from a 250 ml Erlenmeyer flask was added after cooling. The flask was sealed and placed on an orbital shaker at 30°C. The cultures were allowed to grow for 3-5 days. This method is repeated for each microorganism in the mixture.

[0085] In the final outgrowth p...

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Abstract

The invention discloses a microbial preparation based black and odorous water in-situ remediation method and a microbial preparation. The method is a microorganism based combined bioremediation method, and includes the combined application of dredging, construction of ecological revetment, re-aeration, adding of a high efficiency microbial preparation, suspended filler, etc. The microbial preparation is the core of the in-situ remediation method. The microbial preparation with high efficiency degradation ability is added into the water, is composed of different types of microorganisms, relieson the synergistic effect of high density floras for transfer, transformation and degradation of organic pollutants in the water, also decomposes various organic pollutants in the water into carbon dioxide, nitrogen, nitrate, sulfate and the like, and utilizes the food chain of the water ecological communities to form matter and energy cycle, thus enhancing the self-purification ability of the water.

Description

technical field [0001] The invention belongs to the technical field of water environment restoration, and in particular relates to an in-situ repair method for black and odorous water bodies based on microbial preparations and microbial preparations. Background technique [0002] With the rapid development of social economy, the continuous advancement of industrialization and urbanization, the concentrated discharge of industrial wastewater and domestic sewage, the load of organic carbon pollutants, organic nitrogen pollutants and phosphorus-containing compounds in urban rivers continues to increase. Water pollution is becoming more and more serious. After investigation, it was found that organic pollutants widely present in sediments include: polycyclic aromatic hydrocarbons (PAHs), pesticides (such as DDT), chlorinated hydrocarbons (such as polychlorinated biphenyls (PCBs), monocyclic aromatic hydrocarbons (benzene and its Derivatives) and phthalates, etc., organic matter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F3/32C02F11/02C02F101/16C02F101/30
CPCC02F3/32C02F3/34C02F11/02C02F2101/30C02F2101/16
Inventor 黎双飞柳新月杨雪薇陈辉蓉王科举刘良叙
Owner SHENZHEN UNIV
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