Microbial preparation based black and odorous water in-situ remediation method and microbial preparation
A microbial preparation, in-situ remediation technology, applied in biological water/sewage treatment, biological sludge treatment, chemical instruments and methods, etc., can solve the problems of high price, difficult to promote, and biological safety hazards.
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Embodiment 1
[0042] Embodiment 1: In-situ repair of black and odorous water body
[0043] Taking the black and odorous water body of a polluted river in Shenzhen as the restoration object, the main indicators are: the dissolved oxygen (DO) of the overlying water body is 1.8mg / L, the redox potential (ORP) of the sediment is -240mv, and the moisture content of the sediment is 43.7%, the organic matter (TOC) content of the bottom mud is 2.88%, and the total microbial activity of the bottom mud as determined by the FDA method is 0.59A / g.
[0044] The black and odorous water in-situ repair method adopted in this embodiment, the specific steps are as follows:
[0045] S1. Sewage interception: Introduce the running water near the implementation section to the downstream of the implementation section, and prohibit external waste water and domestic garbage from entering the treatment waterway section during the implementation period. Along the width of the waterway, use fishing nets and PE nets to...
Embodiment
[0058] Evaluation of Example Method Application
[0059] In this example, a combined bioremediation method based on microbial preparations is used to build ecological revetments through dredging, aeration and re-oxygenation, adding high-efficiency microbial preparations, and suspended fillers. In-situ restoration, using the self-purification ability of aquatic ecological animals, plants and microorganisms to absorb organic pollutants in the water body, so as to achieve the purpose of water purification.
[0060] ① Dredging to build ecological revetment
[0061] The key to waterway improvement is sewage interception, without which it is impossible to fundamentally improve water quality. Pollution sources of waterways include internal pollution and external pollution: internal pollution generally comes from sediment; external pollution includes domestic sewage, industrial wastewater, rainwater discharge, domestic garbage, dumping of residual mud, etc. The bottom sludge has a h...
Embodiment 2
[0081] Embodiment 2: the preparation of microbial preparation
[0082] The present invention employs microorganisms cultured by standard submerged fermentation methods known in the art.
[0083] ① Bacillus species
[0084] Starter cultures of each of B. subtilis, B. amyloliquefaciens, B. orientalis, and B. natto were grown according to the following general protocol: 18 g of nutrient broth, 18 g of yeast extract, and 36 g of maltodextrin were added to a 1 L Erlenmeyer flask. Add 900ml of distilled deionized water and stir the flask until all dry ingredients are dissolved. The flask was capped and placed in an autoclave operating at 121°C and 15 psi for 30 minutes. After cooling, 100 ml of microbial culture medium from a 250 ml Erlenmeyer flask was added after cooling. The flask was sealed and placed on an orbital shaker at 30°C. The cultures were allowed to grow for 3-5 days. This method is repeated for each microorganism in the mixture.
[0085] In the final outgrowth p...
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