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Method for preparing different crystal forms of calcium carbonate by microbial mineralization

A technology of calcium carbonate and microorganisms, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems affecting the application effect of calcium carbonate, achieve the effects of improving application effect, simple operation, and overcoming high energy consumption

Active Publication Date: 2022-02-15
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are certain differences in the stability, compactness and morphology of calcium carbonate with different crystal forms, which will also affect the application effect of calcium carbonate in practical applications.

Method used

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  • Method for preparing different crystal forms of calcium carbonate by microbial mineralization
  • Method for preparing different crystal forms of calcium carbonate by microbial mineralization
  • Method for preparing different crystal forms of calcium carbonate by microbial mineralization

Examples

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Effect test

Embodiment 1

[0049] A kind of method utilizing microbial mineralization to prepare calcium carbonate of different crystalline forms, the steps are as follows:

[0050] (1) Preparation of culture medium for Bacillus sarcina pasteurii: Weigh 15.75g of Tris and dissolve it in 1L of distilled water, adjust the pH with 50% hydrochloric acid to make a Tris-Hcl buffer solution with a pH of 9.0, then weigh the yeast extract Material 20g, ammonium sulfate 10g, dissolved in 1LTris-Hcl buffer. The prepared liquid medium was divided into Erlenmeyer flasks, sterilized at 121°C for 20 minutes, and then taken out for use.

[0051] (2) Take by weighing 83.3g of anhydrous calcium chloride, 45.0g of urea is dissolved in 1L distilled water, mix well, the concentration of calcium chloride in this mixed solution is about 0.75mol / L, the amount of substance of calcium chloride and urea The ratio is about 1:1.

[0052] (3) Pick a single colony of Bacillus sarcini in the ultra-clean workbench and inoculate it in...

Embodiment 2

[0055] A kind of method utilizing microbial mineralization to prepare calcium carbonate of different crystalline forms, the steps are as follows:

[0056] (3) Preparation of culture medium for Bacillus sarcina pasteurii: Weigh 15.75g of Tris and dissolve it in 1L of distilled water, adjust the pH with 50% hydrochloric acid, configure it as a Tris-Hcl buffer solution with a pH of 9.0, and then weigh the yeast extract Material 20g, ammonium sulfate 10g, dissolved in 1LTris-Hcl buffer. The prepared liquid medium was divided into Erlenmeyer flasks, sterilized at 121°C for 20 minutes, and then taken out for use.

[0057] (4) Take by weighing 83.3g of anhydrous calcium chloride, 45.0g of urea is dissolved in 1L distilled water, mix well, the concentration of calcium chloride in this mixed solution is about 0.75mol / L, the amount of substance of calcium chloride and urea The ratio is about 1:1.

[0058] (3) Pick a single colony of Bacillus sarcini in the ultra-clean workbench and in...

Embodiment 3

[0061] A kind of method utilizing microbial mineralization to prepare calcium carbonate of different crystalline forms, the steps are as follows:

[0062] (1) Preparation of culture medium for Bacillus sarcina pasteurii: Weigh 15.75g of Tris and dissolve it in 1L of distilled water, adjust the pH with 50% hydrochloric acid to make a Tris-Hcl buffer solution with a pH of 9.0, then weigh the yeast extract Material 20g, ammonium sulfate 10g, dissolved in 1LTris-Hcl buffer. The prepared liquid medium was divided into Erlenmeyer flasks, sterilized at 121°C for 20 minutes, and then taken out for use.

[0063] (2) Take by weighing 83.3g of anhydrous calcium chloride, 45.0g of urea is dissolved in 1L distilled water, mix well, the concentration of calcium chloride in this mixed solution is about 0.75mol / L, the amount of substance of calcium chloride and urea The ratio is about 1:1.

[0064] (3) Pick a single colony of Bacillus sarcini in the ultra-clean workbench and inoculate it in...

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Abstract

The invention provides a method for preparing calcium carbonate of different crystalline forms by utilizing microbial mineralization, inoculating the washed and resuspended bacterial suspension into a mixed solution containing calcium salt and urea at a certain ratio, standing and controlling different crystal forms of calcium carbonate. Mineralization reaction conditions: Mineralization reaction is carried out. After the reaction is completed, the precipitated material is taken out for washing and drying to obtain calcium carbonate products of different crystal forms, wherein the microorganism is a high-yield strain of urease, Sarcina pasteurianus. The present invention utilizes microbial mineralization to prepare calcium carbonate of different crystal forms, wherein the utilized microbial resources are abundant, the enzyme-catalyzed reaction is highly efficient, the production process is simple, and the cost is low. By controlling the mineralization reaction conditions, different calcium carbonates with different stability and structural compactness are obtained. The crystalline calcium carbonate sample improves the application effect of the calcium carbonate and makes the calcium carbonate more widely used in many fields.

Description

technical field [0001] The invention relates to a preparation method of calcium carbonate, in particular to a method for preparing calcium carbonate of different crystal forms by utilizing microbial mineralization. Background technique [0002] Calcium carbonate is an inorganic chemical material with a large amount of use and a wide range of uses. It is also a typical biomineralization product and is widely used in different fields such as rubber, plastics, papermaking, inks, coatings, medicine, environmental protection, and food. The crystal forms of calcium carbonate include calcite, aragonite, and vaterite. Among them, calcite has the most stable structure, while aragonite and vaterite are both in an unstable state. Different crystal forms of calcium carbonate also have certain differences in their structure density and morphology. Therefore, the crystal form of calcium carbonate has a certain influence on its application effect. [0003] The commonly used calcium carbon...

Claims

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Application Information

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IPC IPC(8): C12P3/00C12R1/07
CPCC12P3/00
Inventor 韩培培李梦楠张晓琪贾士儒钟成谭之磊
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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