Bilirubin response electrode, preparation method thereof, bilirubin detector containing same and use method

A technology that responds to electrodes and bilirubin. It is used in instruments, measuring devices, scientific instruments, etc. It can solve the problems of decreased dispersion, hindered application, and decreased electron conduction efficiency, and achieve the effect of short response time.

Inactive Publication Date: 2019-02-01
陕西莫泰若传感科技有限公司
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At this time, graphene nanosheets tend to re-stack and graphitize, resulting in a decrease in their electron conduction efficiency; and the dispersion in the solution drops sharply, forming a heterogeneous solution or film, which seriously hinders its use in actual research and commercial products. The application of graphene is one of the problems that need to be solved urgently in the field of graphene research

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bilirubin response electrode, preparation method thereof, bilirubin detector containing same and use method
  • Bilirubin response electrode, preparation method thereof, bilirubin detector containing same and use method
  • Bilirubin response electrode, preparation method thereof, bilirubin detector containing same and use method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] A bilirubin responsive electrode prepared by the following method:

[0076] (1) Preparation Solution: Method: Cool the diethyl ether solution to -78°C, then add TTF powder and lithium diisopropylamide according to the proportion of the reaction chemical formula, and cool it fully; then pass in carbon dioxide gas for 1 hour, continue stirring at low temperature for 12 hours, wash, Filter to obtain carboxylated TTF-COOH powder material;

[0077] (2) Preparation of gold nanoparticle sol by liquid-phase synthesis method: All experimental devices were immersed in aqua regia solution for 12 hours, washed with ultrapure water for 3 times, and placed for use. 2 mL of 1.0% HAuCl 4 Solution and 5mL of 1% sodium citrate solution were mixed and stirred, and diluted with 200mL of ultrapure water into a 400ml beaker; after uniform stirring, the color of the solution changed from blue to wine red; after that, the solution was continued to be heated After 10 minutes, remove the hea...

Embodiment 2

[0093] A bilirubin responsive electrode, the difference between the preparation method and Example 1 is: the concentration of gold nanoparticles in the TTF-COOH / G dispersion is 0.4‰mg / mL, and the concentration of TTF-COOH / G is 0.5mg / mL .

Embodiment 3

[0095] A bilirubin responsive electrode, the difference between the preparation method and Example 1 is: the concentration of gold nanoparticles in the TTF-COOH / G dispersion is 0.6‰mg / mL, and the concentration of TTF-COOH / G is 1.2‰mg / mL mL.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
concentrationaaaaaaaaaa
concentrationaaaaaaaaaa
Login to view more

Abstract

The invention provides a bilirubin response electrode. The electrode includes an electrode body. A graphene composite material, a biocompatible material and a bilirubin specific-recognition enzyme areadsorbed at the electrode. The graphene composite material includes a tetrathiafulvalene derivative and graphene dispersed in the tetrathiafulvalene derivative. The tetrathiafulvalene derivative hasa structure of a formula (I). The designed bilirubin response electrode of the invention utilizes changes of electrical signals after bilirubin oxidase and bilirubin binding reaction to obtain bilirubin content data, and response time is short.

Description

technical field [0001] The invention belongs to the technical field of electrochemical sensors, and in particular relates to a bilirubin responsive electrode, a preparation method thereof, a bilirubin detector comprising the same and a use method thereof. Background technique [0002] Bilirubin is an important basis for clinically judging jaundice and an important indicator of liver function. It is very important to efficiently, quickly and accurately determine its content in human blood. At present, the methods for measuring serum bilirubin mainly include diazonium reagent method, bilirubin oxidase method, vanadate oxidation method, etc., all of which are based on the chemical reaction phenomenon of bilirubin and other reagents and the change of absorbance to determine its content in detail. Measured. [0003] CN 106896102A discloses a total bilirubin detection reagent, the composition of which is: preservative 6-8wt%; Tris-HCl buffer solution 160-180mmol / L; sodium lauryl ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30G01N27/48
CPCG01N27/308G01N27/3271G01N27/3277G01N27/48
Inventor 冯晴亮朱美洁刘宏燕郑建邦
Owner 陕西莫泰若传感科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap