Method for preparing nanocluster gel of hydroxyl-like phosphorite component

A hydroxyapatite-like and nano-cluster technology is applied in non-active ingredients medical preparations, inorganic non-active ingredients, pharmaceutical formulations, etc., and can solve the problems of complicated preparation steps, high cost, poor biocompatibility, etc. Achieve the effect of low cost, high yield and excellent degradable properties

Active Publication Date: 2019-02-05
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the preparation steps of the existing fluorescent gel are relatively complicated, the biocompatibility is not good, and the cost is high.

Method used

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  • Method for preparing nanocluster gel of hydroxyl-like phosphorite component
  • Method for preparing nanocluster gel of hydroxyl-like phosphorite component
  • Method for preparing nanocluster gel of hydroxyl-like phosphorite component

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 AuATP-Ca

[0024] (1) Preparation of precursor gold clusters of AuATP gel: Weigh 200 μL of 0.1 mol / L chloroauric acid solution and 4 mL of 10 mmol / L adenosine triphosphate, mix the two well, and allow them to fully react for 15 minutes (the two are mixed The color of the final solution is light yellow to nearly colorless), the ratio of gold to adenine nucleotide series is 1:2; then add ultrapure water to the mixed solution, set the volume to 15mL, after 0.5h, add 0.2M 5mL of citric acid buffer solution, the molar ratio of citric acid to gold is 50:1; Mix 20mL of absolute ethanol with the same volume as the above reaction solution, mix and shake well; finally, centrifuge 40mL of the turbid solution in a centrifuge at 8000rpm for 15min to precipitate, take out the precipitate and dry it overnight, and weigh the sample after overnight drying, according to Proportionally add ultra-pure water and mix to form a solution of 10 μg / mL, and store it in freezer at -20°C...

Embodiment 2

[0028] Example 2 AuAMP-Ca

[0029] The basic steps are the same as in Example 1, except that adenosine monophosphate is used for the adenine nucleotide series.

Embodiment 3

[0030] Example 3 AuADP-Ca

[0031] The basic steps are the same as in Example 1, except that adenosine diphosphate is used in the adenine nucleotide series.

[0032] Cytotoxicity Assay

[0033] HepG2 cells in the logarithmic phase of rapid growth and normal liver cells L02 were selected, collected after trypsinization, added 100 μL of medium to each well of a 96-well plate, and inoculated at a density of 10,000 cells / well. The cells were placed in a constant temperature incubator with 5% carbon dioxide concentration at 37°C and cultured for 10 hours to complete attachment. Add AuAMP-Ca, AuADP-Ca and 100 μL of AuATP-Ca medium, after continuing to culture for 24 h, add 20 μL of 5 mg / mL MTT solution to each well, continue to culture for 4 h, discard the supernatant in the well, add 150 μL of dimethyl sulfoxide to each well, Shake horizontally for 15 minutes to completely dissolve the MTT product crystals in the wells. Use a microplate reader to measure the light absorption va...

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Abstract

The invention discloses a method for preparing nanocluster gel of a hydroxyl-like phosphorite component. The method comprises the following steps: mixing a chloroauric acid solution and a adenine nucleotide series, reacting for 1 to 60 minutes, adding a citric acid buffering solution, standing for 0.5 to 24 hours in a light-shielding manner, adding absolute ethyl alcohol, uniformly mixing, and centrifuging to obtain precipitates, preparing the obtained precipitates into an adenine nucleotide shell nanocluster with the concentration of 0.1 to 10000 microgram/mL, adding metal ions to prepare sediments, dispersing the sediments in deionized water, thus obtaining the nanocluster gel. The fluorescent gold nanocluster adopting the adenine nucleotide series as ligand reacts with the metal ions toobtain aggregation-induced fluorescent nanocluster gel, the aggregation of a metal-phosphate structure achieves the fluorescent reinforced effect, the combination force of gold and adenine in the nanocluster gel is slightly weak, can be dissociated under the existence of molecules such as glutathione in the cell, the degradability is excellent, and the preparation method is simple in operation, high in yield, and low in cost.

Description

technical field [0001] The invention belongs to the field of gel preparation, in particular to a method for preparing nano-cluster gel of hydroxyapatite-like components. Background technique [0002] In recent years, gold nanomaterials have been widely used in many fields such as the development of new biosensors, in vivo or in vitro imaging of organisms, early diagnosis and treatment of diseases, etc. due to their superior characteristics. Using natural biological small molecules as precursor materials, the application of green chemical methods to synthesize metal nanomaterials has outstanding advantages. The adenine nucleotide series originates from living organisms, has the characteristics of stable chemical properties and good biocompatibility, and is favored in the fields of biomedicine and other fields. It can be used for macro-preparation of metal nanostructures and has good industrialization prospects. Among them, fluorescent gold nanoclusters with ultra-small size...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K9/06A61K47/02A61K47/26
CPCA61K9/06A61K47/02A61K47/26A61K49/0017A61K49/0052A61K49/0065A61K49/0073
Inventor 姜晖刘浏秦肇建王雪梅
Owner SOUTHEAST UNIV
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