Confocal microscopy imaging system for fluorescence lifetime in second near-infrared region

A fluorescence lifetime and microscopic imaging technology, applied in fluorescence/phosphorescence, material analysis through optical means, measurement devices, etc., to achieve the effects of easy disassembly, enhanced sensitivity, and rich image information

Inactive Publication Date: 2019-02-12
ZHEJIANG UNIV
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  • Application Information

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Problems solved by technology

However, there are few commercially available TCSPC microscop

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  • Confocal microscopy imaging system for fluorescence lifetime in second near-infrared region
  • Confocal microscopy imaging system for fluorescence lifetime in second near-infrared region
  • Confocal microscopy imaging system for fluorescence lifetime in second near-infrared region

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Embodiment Construction

[0024] The present invention will be further described below in conjunction with accompanying drawing.

[0025] The present invention utilizes functionally independent hardware equipment and time-correlated single photon counting (TCSPC) technology to build a set of confocal near-infrared second region (1000-1700nm) fluorescence lifetime microscopy imaging system based on single-photon excitation. It is based on the laser scanning confocal microscope (FV1000+BX61), uses 810 nm femtosecond pulsed laser as the light source to excite the sample, and introduces the near-infrared second region fluorescence of the sample into the near-infrared through the near-infrared anti-reflection large-diameter optical fiber The photomultiplier tube (PMT) (H12397-75) with the response of the second zone band is converted into an electrical signal, and the Becker&Hickl counting board (SPC-150) is used to realize the near-infrared second zone fluorescence lifetime under confocal conditions by usin...

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Abstract

The invention discloses a confocal microscopy imaging system for the fluorescence lifetime in a second near-infrared region. A laser emitted by a femtosecond laser source is introduced into an uprightconfocal scanning microscope. The laser is focused on the sample by a near-infrared enhanced objective lens, after pass through a scanning galvanometer, a scanning lens, and a sleeve lens. The signals in the second near-infrared region of the sample are collected by a same near-infrared enhanced objective lens; are split by a long-pass dichroic mirror and then are taken out of the system, after pass through a sleeve lens, a scanning lens, and a scanning galvanometer; are collected and coupled into a large diameter fiber; and finally are converted into electrical signals by a photomultiplier tube detection. The electrical signals are converted into electrical counting signals, and input to a counting board. At the same time, the counting board receives synchronous pulse signals from an excitation light, and uses the signals as timing stop signals. The system obtains each sample of a fluorescence lifetime image in the second near-infrared region and a fluorescence intensity image in thesecond near-infrared region, after processed by the computer. According to the confocal microscopy imaging system for the fluorescence lifetime in the second near-infrared region, the system expandsa function of the fluorescence lifetime imaging in the second near-infrared region; and obtains more abundant image information.

Description

technical field [0001] The invention belongs to the field of microscopic imaging of applied optics, and relates to a confocal near-infrared second-zone fluorescence lifetime microscopic imaging system. Background technique [0002] 1. Confocal near-infrared two-region fluorescence microscopy imaging based on single-photon excitation [0003] According to the relevant theory of the biological tissue window, the fluorescence in the second near-infrared region (1000-1700nm) has a small scattering in biological tissue and has a strong tissue penetration ability, which is conducive to the realization of large depth and high (spatial) resolution imaging. In addition, the autofluorescence of biological tissues in this band is low, so near-infrared second region fluorescence imaging also has the advantage of a high signal-to-background ratio. [0004] At present, commercial near-infrared second-region fluorescence macroscopic imaging systems have appeared on the market, which can r...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6458
Inventor 钱骏虞文斌周静
Owner ZHEJIANG UNIV
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