Nutritional prophylactic for turbot enteritis

A nutritional and preventive technology, applied in the direction of organic active ingredients, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve problems such as fish intestinal inflammation, increase the height of intestinal villi, reduce infiltration, alleviate the effect of

Active Publication Date: 2019-02-15
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a large number of studies have shown that plant protein sources can only replace fish meal in a small amount in carnivorous fish feed, and the replacement of fish meal by plant protein sources will cause intestinal inflammation in fish

Method used

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  • Nutritional prophylactic for turbot enteritis
  • Nutritional prophylactic for turbot enteritis
  • Nutritional prophylactic for turbot enteritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Screening of nutritional preventive agents

[0022] With fishmeal as the main protein source, wheat flour as the main sugar source, and fish oil as the main fat source, the whole fishmeal feed for turbot was prepared as the basic feed. Three protective agents, glutamine (G), mannooligosaccharide (M) and sodium butyrate (S), were combined and added to the basal feed, and experimental feeds with 7 preventive agents were designed, abbreviated as G, M, S, G+M, G+S, M+S, and G+M+S. In addition, 1.0% soybean saponin was added to the basic feed as the feed that caused turbot enteritis, see Table 1 for details. The breeding facilities use 300L fiberglass barrels, and 30 turbot fry are stocked in each barrel, with a starting weight of about 8.5 grams. Each barrel was used as a replicate, and each feed was set up three replicates. A total of 8 experimental treatments were designed, and the basal feed and 7 kinds of feeds were fed to 8 treatments of turbot, and after...

Embodiment 2

[0036] Example 2 Screening of the ratio of the preventive agent for turbot enteritis

[0037]With fishmeal as the main protein source, wheat flour as the main sugar source, and fish oil as the main fat source, the whole fishmeal feed for turbot was prepared as the basic feed. Manno-oligosaccharide (M) and sodium butyrate (S) were added to the basal feed at the ratio of 1:2, 1:3 and 1:5, the total amount added was 1.2%, and three kinds of preventive agents were designed. Experimental feeds are abbreviated as M+S(1:2), M+S(1:3) and M+S(1:5) respectively. In addition, 1.0% soybean saponin was added to the basic feed as the feed that caused turbot enteritis, see Table 4 for details. The breeding facilities use 300L fiberglass barrels, and 30 turbot fry are stocked in each barrel, with a starting weight of about 8.5 grams. Each barrel was used as a replicate, and each feed was set up three replicates. A total of 4 experimental treatments were designed, and the basal diet and 3 k...

Embodiment 3

[0048] Example 3 Effect Evaluation and Concentration Screening of Nutritional Preventive Agent for Turbot Enteritis in Soybean Meal as Protein Source Feed

[0049] With fishmeal as the main protein source, wheat flour as the main sugar source, and fish oil as the main fat source, the whole fishmeal feed for turbot was prepared as the basic feed. Mannan oligosaccharide (M) and sodium butyrate (S) were combined in a ratio of 1:3, added to the basic feed, and the added concentration was 0.5%, 1.0% and 1.5%, and three preventive agents were designed The experimental feeds are abbreviated as MS0.5, MS1.0 and MS1.5 respectively. In addition, 40% soybean meal was added to the basic feed instead of fish meal as the feed for the soybean meal group, see Table 6 for details. The breeding facilities use 300L fiberglass barrels, and 30 turbot fry are stocked in each barrel, with a starting weight of about 8.5 grams. Each barrel was used as a replicate, and each feed was set up three repl...

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Abstract

The invention provides a nutritional prophylactic for turbot enteritis. The nutritional prophylactic contains 20%-30% by mass of mannan oligosaccharide and 70%-80% by mass of sodium butyrate. The invention further provides an application of the nutritional prophylactic to preparation of turbot feed. The nutritional prophylactic for turbot enteritis is used before feeding with feed containing turbot enteritis-inducing anti-nutritional factors and plant protein sources, and can remarkably reduce occurrence of turbot enteritis. Specifically, the nutritional prophylactic can remarkably increase intestinal villus height, reduce intestinal villus adhesion, reduce infiltration of inflammatory cells on lamina propria and submucosa and increase vacuoles absorbed on epithelia cell nuclei. Compared with antibiotics for treatment, the nutritional prophylactic for turbot enteritis is safer and more reliable.

Description

technical field [0001] The invention belongs to the technical field of aquaculture feed, in particular to a nutritional preventive agent for turbot enteritis. Background technique [0002] Fish provides high-quality protein sources for humans, which is very important for improving dietary nutritional structure and ensuring human health. With the development of society, the improvement of people's living standards and the increasing awareness of health, the demand and consumption of fish products have increased dramatically. However, while fish farming is booming, the problems of water pollution and ecological environment damage are becoming increasingly prominent, resulting in frequent outbreaks of fish diseases. The reason is that fish live in water and are lower vertebrates with weak digestion and disease resistance. Among them, intestinal health is the key to healthy fish farming. The intestinal tract is the organ with the largest contact area between fish and the exter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/702A61P1/00A23K50/80A23K20/163A23K20/105A61K31/19
CPCA61K31/19A61K31/702A23K20/105A23K20/163A23K50/80A61P1/00A61K2300/00Y02A40/818
Inventor 谷珉潘世会白楠贾倩柳文爽
Owner SHANDONG UNIV
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