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Method for producing high-quality high-yield selenium-enriched hericium erinaceus culture

A production method and technology of Hericium erinaceus, applied in the directions of using microorganisms, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of long production cycle, high cost and low yield of Hericium erinaceus, and achieve rich The effect of selenium is remarkable, the production cycle is short, and the output is high

Active Publication Date: 2019-02-15
上海紫京生物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a kind of method that utilizes liquid fermentation technology to produce high-quality and high-yield selenium-enriched Hericium erinaceus strains, thereby solving the problems of long production cycle, low efficiency, low output and high cost of Hericium erinaceus in the prior art, and at the same time Increasing the content of organic selenium in Hericium erinaceus will help meet the needs of industrialized cultivation of Hericium erinaceus and selenium-rich products on the market

Method used

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  • Method for producing high-quality high-yield selenium-enriched hericium erinaceus culture
  • Method for producing high-quality high-yield selenium-enriched hericium erinaceus culture
  • Method for producing high-quality high-yield selenium-enriched hericium erinaceus culture

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Test according to the production process of the high-quality and high-yield selenium-rich Hericium erinaceus bacterial classification provided by the invention, the steps are as follows:

[0040] Step 1. Make the mother species

[0041] Screen the excellent strain "Changshan Hericium" of Hericium erinaceus with fast mycelial growth and strong growth. Under sterile conditions, use an inoculation shovel to take out a piece of Hericium erinaceus with a size of about 0.5cm. 2 Inoculate the slant of the PDA-enriched medium, culture at 25°C for 6 days to obtain the slant strain, then transfer the slant strain to the PDA-enriched medium plate, and cultivate at 25°C until the mycelia cover the plate. Get the mother species. The composition of PDA rich medium is: potato 200g / L, trehalose 20g / L, agar 20g / L, peptone 3.5g / L, lemon juice 4mL / L, wort juice 12mL / L, VB 1 25mg / L, VB 2 25mg / L, MgSO 4 ·7H 2 O0.5g / L, KH 2 PO 4 1.0g / L, the balance is water, and the pH is adjusted ...

Embodiment 2

[0053] In order to compare the liquid culture medium of the present invention and the influence of conventional liquid culture medium on Hericium erinaceus shake flask strain growth, the present embodiment replaces the liquid culture medium adopted in embodiment 1 step 2 with the following conventional culture medium: culture medium A (glucose 30g / L, yeast extract 10g / L, KH 2 PO 4 1g / L, MgSO 4 ·7H 2 O0.5g / L), medium B (soluble starch 25g / L, yeast extract 25g / L, KH 2 PO 4 1g / L, MgSO 4 ·7H 2 O1g / L), medium C (maltose 20g / L, wheat bran 15g / L, peptone 20g / L, yeast powder 2g / L, KH 2 PO 4 1.5g / L, MgSO 4 ·7H 2 (00.75g / L), other conditions are constant, measure the mycelia biomass, bacterium ball diameter and bacterium ball density of shake flask bacterial classification, and statistics are as follows table 1 together with the result of embodiment 1.

[0054]

[0055] As can be seen from the results in Table 1, when the liquid culture medium of the present invention wa...

Embodiment 3

[0057] 1) In order to prove the positive effect of electric field and magnetic field co-treatment on selenium-enriched fermentation strains, the prepared shake flask strains were subjected to the following treatments in this example: 1) electric field treatment alone; 2) magnetic field treatment alone; 3) Without treatment, the mycelium biomass, total selenium content and organic selenium content of the fermented strains were measured, and the results of Example 1 were counted in Table 2 below.

[0058]

[0059] As can be seen from the results in Table 2, compared with no treatment, the mycelium biomass, total selenium content and organic selenium content of Hericium erinaceus fermentation strains were all the same when the electric field and magnetic field were jointly treated, the electric field was treated alone, and the magnetic field was treated alone. got improved. Compared with a single treatment method, the treatment effect of electric field and magnetic field is si...

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Abstract

The invention provides a method for producing high-quality high-yield selenium-enriched hericium erinaceus culture and belongs to the field of production of edible mushrooms. The method comprises thefollowing process steps: 1, making stock culture; 2, making shake flask culture; 3, performing electric field and magnetic field co-treatment on the shake flask culture; 4, producing selenium-enrichedfermentation culture. According to the method disclosed by the invention, the hericium erinaceus culture is produced by utilizing a liquid fermentation technology, the solid production method of thetraditional hericium erinaceus culture is changed, the culture production cycle is shortened, the quality and yield of the culture are improved, and the cost is reduced. Meanwhile, selenium is added into the liquid fermentation process, and then the selenium-enriched hericium erinaceus culture having the organic selenium content of 87.84mg / kg is obtained. The culture is fast in growth after inoculation, strong in growth vigor and high in yield, the organic selenium content in the produced hericium erinaceus fruiting bodies reaches 87.84mg / kg, and the culture has extremely high market value.

Description

technical field [0001] The invention belongs to the field of edible fungus production, and relates to a production method of high-quality and high-yield selenium-enriched Hericium erinaceus strains, in particular to a method for producing selenium-enriched Hericium erinaceus strains by using liquid fermentation technology. Background technique [0002] Hericium erinaceus ( Hericium erinaceus ) is also called Hericium erinaceus, Hedgehog, Cauliflower or Mountain bacterium, etc. It belongs to Basidiomycota, Laminomycetes, Phyllomycetes, Dendtaceae, and Hericium genus. Because of its round and thick fruiting body, it often hangs in the The trunk is covered with needle-like thorns, which are shaped like a monkey's head, hence the name Hericium erinaceus. The meat of Hericium erinaceus is fresh and tender, mellow and delicious. It is known as "vegetarian meat". It is also known as the four traditional Chinese dishes together with bear's paw, sea cucumber and shark's fin. Herici...

Claims

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Application Information

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IPC IPC(8): C12N13/00C12N1/14C12N1/36C12R1/645
CPCC12N1/14C12N1/36C12N13/00
Inventor 沙淼高雪谷玉秋
Owner 上海紫京生物科技有限公司
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