Method for biodegrading vomitoxin in DDGS

A production method and microbial fermentation technology, applied in the fields of bacteria, food science, and application used in food preparation, can solve the problems of research and development of feed additives, and no research reports on deoxynivalensis degrading enzymes.

Inactive Publication Date: 2019-02-19
SHANGHAI HONGYUAN BIOTECH
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  • Abstract
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  • Application Information

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Problems solved by technology

But so far, there is no research report on the deoxygenation enzyme of vomitoxin, and there is no rel

Method used

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Embodiment Construction

[0012] Using the production method of Lactobacillus acidophilus fermentation material, the production method of Bacillus subtilis fermentation material, the production method of Saccharomyces cerevisiae fermentation material and the fermentation of Aspergillus niger disclosed in "Microbiological Fermentation Antibiotic-Free Feed and Its Preparation Method" with the patent number ZL 201010148491.6 The production method of the material, the production method of the fermented material of Aspergillus oryzae, and the "self-supporting keyboard" disclosed by my patent number ZL201220624896. Kind of koji and Aspergillus oryzae kind of koji for use.

[0013] Utilize described ZL 201010148491.6 making fermented material method to make Lactobacillus acidophilus kind of koji, Bacillus subtilis kind of koji, Saccharomyces cerevisiae kind of koji, Aspergillus niger kind of koji, Aspergillus oryzae kind of koji comprising the following steps:

[0014] (1) Making Lactobacillus acidophilus koj...

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Abstract

The invention discloses a method for effectively biodegrading vomitoxin in DDGS. The method comprises the following steps: mixing 15-25% of two or one of a lactobacillus acidophilus mould starter anda saccharomyces cerevisiae mould starter, 65-45% of two or one of an aspergillus niger mould starter and an aspergillus oryzae mould starter and 20-30% of a bacillus subtilis mould starter into a composite mould starter; inoculating the composite mould starter into DDGS according to 3-5% of inoculating amount, after stirring for 30 min, adding an aqueous solution which contains 3.0-4.2% of ammonium sulfate until the water content of the material reaches 42-48%, stirring uniformly, standing for fermenting for 4-20 h, when the material layer is within 30 cm and the temperature rises to be 36-38DEG C, carrying out turning (oxygenation) cooling to the temperature of 30-27 DEG C, and then stopping turning; when the material layer is within 30 cm and the temperature rises to be 36-38 DEG C again, carrying out turning (oxygenation) cooling to be temperature of 30-27 DEG C again, and then stopping turning; repeatedly operating, and maintaining the whole process for 36-48 h; and when the material temperature does not rise sharply, carrying out turning cooling on the fermented material to the temperature of 26-28 DEG C, flattening and compacting the material, and then sealing for 20-30 h, and stopping fermentation and degradation when a fermentation tank diffuses sour fragrance.

Description

technical field [0001] The invention relates to the technical field of biodegradation of mycotoxins polluted by grain and oil, in particular to the biodegradation technology of vomitoxin polluted by DDGS, a by-product of corn deep processing. Background technique [0002] According to the estimates of the Food and Agriculture Organization of the United Nations, the losses caused by mycotoxin pollution in the world amount to hundreds of billions of dollars every year, and the harm to humans and animals is even more difficult to count. In recent years, the negative impact of mycotoxin pollution on livestock production has gradually been recognized, and foreign scientific research institutions have begun to carry out a series of studies on the biodegradation of mycotoxins. In China, there are few research reports on DON-degrading bacteria and detoxification enzymes. Screening strains capable of producing DON-degrading enzymes and obtaining highly expressed detoxification enzyme...

Claims

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Application Information

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IPC IPC(8): A23L5/20
CPCA23L5/28A23V2002/00A23V2400/113A23V2250/762
Inventor 王德昌张玉国张楠楠
Owner SHANGHAI HONGYUAN BIOTECH
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