Extracting method of cowhide collagen

An extraction method and original protein technology, which is applied in the field of bovine collagen extraction, can solve the problems of harsh extraction temperature, high collagen loss, restricting the development of enzymatic methods, etc. The effect of shortening the extraction time

Active Publication Date: 2019-02-19
GUIZHOU MOUNT FANJING ECOLOGICAL AGRI INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the hair removal and degreasing process of the prior art causes a lot of collagen loss, and the pre-extraction treatment generally first removes the hair and then degreases, which increases the process and requires harsh extraction temperatures, which limits the development of enzymatic methods, and most researchers are committed to How to optimize the extraction effect of enzyme combination, but there are few studies on how to reduce the loss rate of collagen in the process of hair removal and degreasing and how to reduce the extraction temperature requirements

Method used

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  • Extracting method of cowhide collagen
  • Extracting method of cowhide collagen

Examples

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Effect test

Embodiment 1

[0033] A method for extracting cowhide collagen, comprising the steps of:

[0034] S1 degreasing and hair removal: immerse the cowhide in water 2.5 times the weight of the cowhide, control the temperature at 40°C, add a hair removal and degreasing compound enzyme preparation with a weight of 0.002 cowhide, treat at a constant temperature for 3 hours, drain, and wash with water;

[0035] S2 Immunization treatment: Put the cowhide in a sodium carbonate solution 1.5 times the weight of the cowhide, maintain the pH at 8.5, and keep the temperature at 25°C. After rotating for 20 minutes, take it out and drain it, add water 1.3 times the weight of the cowhide, and heat it at 600 rpm. Stir at a high speed under the condition of 1 min to make a paste, and process it with a high-pressure homogenizer to make a kraft pulp;

[0036] S3 extraction: Add organic acid solution to the cowhide pulp after immune treatment to adjust the pH to 6.8, then add an activated compound enzyme preparation...

Embodiment 2

[0048] A method for extracting cowhide collagen, comprising the steps of:

[0049] S1 degreasing and hair removal: immerse the cowhide in water twice the weight of the cowhide, control the temperature at 35°C, add a hair removal and degreasing compound enzyme preparation with a weight of 0.001 cowhide, treat at a constant temperature for 2 hours, drain, and wash with water;

[0050] S2 immune treatment: place cowhide in a sodium carbonate solution 1.2 times the weight of cowhide, keep the pH at 7.8, and keep the temperature at 20 °C. Stir at a high speed under the condition of 1 min to make a paste, and process it with a high-pressure homogenizer to make a kraft pulp;

[0051] S3 extraction: Add organic acid solution to the cowhide pulp after immune treatment to adjust the pH to 6, then add an activated compound enzyme preparation 0.002 times the weight of cowhide, stir at 100r / min for 5h at natural temperature, and use a filter press to solidify The liquid is separated to ob...

Embodiment 3

[0063] A method for extracting cowhide collagen, comprising the steps of:

[0064] S1 degreasing and hair removal: immerse the cowhide in water 2.2 times the weight of the cowhide, control the temperature at 37°C, add a hair removal and degreasing compound enzyme preparation with a weight of 0.0012 cowhide, treat it at a constant temperature for 2.5 hours, drain it, and wash it with water;

[0065] S2 immune treatment: place cowhide in a sodium carbonate solution 1.3 times the weight of cowhide, keep the pH at 8.0, and at a temperature of 25°C, turn it for 18 minutes, take it out and drain it, add water 1.2 times the weight of cowhide, and heat at 550 rpm Stir at a high speed under the condition of 1 min to make a paste, and process it with a high-pressure homogenizer to make a kraft pulp;

[0066] S3 extraction: Add organic acid solution to the cowhide pulp after immune treatment to adjust the pH to 6.5, then add an activated compound enzyme preparation 0.003 times the weight...

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Abstract

The invention relates to the technical field of biological collagen preparation, in particular to an extracting method of cowhide collagen. The method includes the following steps of S1, conducting degreasing and unhairing, wherein cowhide is soaked in water, an unhairing and degreasing compound enzyme preparation is added under a controlled temperature for constant-temperature treatment, and thecowhide is scooped up and cleaned with clear water; S2, conducting immune treatment, wherein the cowhide is placed in a sodium carbonate solution, the cow hide is rotated for 15-20 min and taken out and drained at the pH of 7.8-8.5 and the temperature of 20-25 DEG C, water is added to be stirred at a high speed to prepare slurry, and cowhide slurry is prepared after the slurry is processed througha high-pressure homogenizer; S3, conducting extracting, wherein an organic acid solution is added to the cowhide slurry to adjust the pH, an active compound enzyme preparation is added, and solid-liquid separation is conducted to obtain an extraction solution for use after stirring is conducted for 5-7 h at a natural temperature; S4, conducting purifying, wherein the extraction solution is centrifugally separated to obtain upper layer liquid, and a collagen solution is obtained. By means of the method, the purity and yield of collagen protein are improved, and the extraction time is shortened.

Description

technical field [0001] The invention relates to the technical field of biological collagen preparation, in particular to a method for extracting cowhide collagen. Background technique [0002] Collagen is a natural biopolymer synthesized by animal cells. It is widely found in animal skin, bone, cartilage, teeth, tendons, ligaments and blood vessels. The role of the body. Collagen has a stable nature, and it is difficult to decompose it under general processing temperature and short-term heating, so it is difficult to be absorbed by the human body, and the utilization rate of food is low. [0003] At present, the commonly used extraction methods include enzymatic degradation, acid extraction, alkali extraction and recombinant DNA extraction. The acid-base method is not suitable for industrial application due to the violent reaction of strong acid and strong alkali and serious corrosion of equipment. The extraction effect of recombinant DNA is good, but the process is compl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/14
CPCC07K14/78C12P21/06
Inventor 蒋宇王小清
Owner GUIZHOU MOUNT FANJING ECOLOGICAL AGRI INC
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