A nanoparticle for improving tumor tissue permeability, its preparation method and application

A nanoparticle, tumor tissue technology, applied in biochemical equipment and methods, antitumor drugs, nanotechnology, etc., can solve problems such as accelerating tumor deterioration and difficulty

Inactive Publication Date: 2020-12-04
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, high intratumoral pressure will increase the difficulty of intratumoral injection, and repeated tumor injection stimulation will also accelerate tumor deterioration, thus limiting the application of this method

Method used

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  • A nanoparticle for improving tumor tissue permeability, its preparation method and application
  • A nanoparticle for improving tumor tissue permeability, its preparation method and application
  • A nanoparticle for improving tumor tissue permeability, its preparation method and application

Examples

Experimental program
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preparation example Construction

[0033] The present invention also provides a method for preparing nanoparticles for improving tumor tissue permeability described in the above technical solution, comprising the following steps:

[0034] (1) dissolving the phase change material, the tumor matrix degrading material and the antitumor drug in methanol to obtain a solution of the loaded material;

[0035] (2) After mixing the solution of the loading material and the hollow mesoporous Prussian blue nanoparticles, stir at 50-80°C for 2-6 hours, then mix the mixed solution obtained by the stirring with water, and then centrifuge to obtain Nanoparticles for enhanced tumor tissue permeability.

[0036] The invention dissolves the phase change material, the tumor matrix degradation material and the antitumor drug in methanol to obtain the solution of the loaded material. In the present invention, there is no special limitation on the order of adding the phase change material, the tumor matrix degradation material and t...

Embodiment 1

[0059] (1) Preparation of Mesoporous Prussian Blue (MPB): Dissolve 3.0 g of polyvinylpyrrolidone and 132 mg of potassium ferricyanide in 40 mL of hydrochloric acid with a concentration of 0.01 M, and stir at room temperature for 30 min; when the solution turns into a light yellow transparent liquid, The solution was placed in a high-pressure reactor and reacted in an electric oven at 80°C for 24 hours; after the reaction was completed, a solid product was obtained by centrifugation, and the solid product was freeze-dried to obtain MPB;

[0060] (2) Preparation of hollow mesoporous Prussian blue (HMPB): 20 mg MPB and 200 mg PVP were dissolved in 20 mL of 1 M hydrochloric acid and stirred at room temperature for 3 h; React in an electric oven for 3h; after the reaction is completed, the product obtained from the reaction is centrifuged to obtain a solid product, and the solid product is freeze-dried to obtain HMPB;

[0061] (3) Preparation of nanoparticles for improving tumor ti...

Embodiment 2

[0066] (1) prepare hollow mesoporous Prussian blue nanoparticles according to the method described in step (1) and step (2) in Example 1;

[0067] (2) Dissolve 50 mg of lauric acid, 2.0 mL of adriamycin methanol solution with a concentration of 4.0 mg / mL and 2.0 mL of methanol solution of losartan with a concentration of 3 mg / mL in methanol, and stir for 30 minutes to obtain a solution of the encapsulation material ;

[0068] Add 2.0 mL of methanol dispersion of hollow mesoporous Prussian blue nanoparticles with a concentration of 3 mg / mL to the solution of the encapsulation material, and stir at 70°C for 4 h; after the stirring is completed, add 800 μL of water, and then centrifuge at 5000 rpm for 10 min , keep the lower layer as the product obtained by centrifugation; the product obtained by the centrifugation is dispersed in deionized water, and then freeze-dried at -50°C for 24h to obtain nanoparticles (abbreviated as (DOX+Losartan)@ HMPB).

[0069] Dissolve the obtained...

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Abstract

The invention belongs to the technical field of medicines for treating tumors, and provides a nano-particle for improving tumor tissue penetrability. The nano-particle comprises hollow mesoporous Prussian blue nano-particles, phase-change materials, tumor matrix degradation materials and anti-tumor medicines, the phase-change materials are loaded in the hollow portions of the hollow mesoporous Prussian blue nano-particles, the tumor matrix degradation materials and the anti-tumor medicines are embedded into the phase-change materials, and the phase-change materials comprise lauric acid, 9-heptadecanone and decanoic acid or 1-tetradecyl alcohol. According to the nano-particle for improving the tumor tissue penetrability, the anti-tumor medicines can enter tumor tissues, and the anti-tumor rate of the nano-particle can reach 81.3%.

Description

technical field [0001] The invention relates to the technical field of medicine for treating tumors, in particular to a nanoparticle for improving the permeability of tumor tissue, its preparation method and application. Background technique [0002] Cancer is the primary chronic disease that threatens human health, and chemotherapy is the basic method for clinical treatment of tumors. Due to the advantages of long blood circulation, selective tumor enrichment, long residence time in tumor sites, and low toxicity, nanomedicine is expected to overcome the shortcomings of small molecule chemotherapy drugs and improve its antitumor effect. However, the average amount of nanoparticles reaching the tumor site is only 0.7% of the injected dose, and most of the nanoparticles can only stay around the blood vessels and cannot enter the center of the tumor, which greatly limits the therapeutic effect of nanomedicine. For example, Professor Liang Xingjie studied the penetration perfor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/06A61K38/47A61K38/48A61K31/337A61K31/4745A61K31/704A61K31/4178A61K47/69A61P35/00B82Y5/00
CPCA61K31/337A61K31/4178A61K31/4745A61K31/704A61K38/47A61K38/4873A61K38/4886A61K45/06A61K47/6949A61P35/00B82Y5/00C12Y302/01035C12Y304/22004C12Y304/24A61K2300/00
Inventor 袁直张雅佩
Owner NANKAI UNIV
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