Helicobacter pylori culture method
A technology of Helicobacter pylori and its culture method, which is applied in the field of deep expansion culture of Helicobacter pylori, can solve the problems of high cost, difficult to cultivate in large quantities, and failure to meet the company's production requirements, and achieve low production costs, good bacterial growth, and reduced culture costs Effect
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Embodiment 1
[0026] The culture method of Helicobacter pylori, comprises the steps:
[0027] 1. Preparation of cryovial B:
[0028] (1) Weigh 1.4 g of Brooke’s Broth, add distilled water to prepare 50 ml of solution, put it into a 100 ml suction filter bottle 1, and sterilize it by high-temperature steam to obtain liquid medium A; take the frozen tube of preserved Helicobacter pylori , after natural melting, the pipette gun draws 2 ml of bacterial solution and inoculates it into sterilized liquid medium A, adds 4 ml of serum, plugs it with a rubber stopper, wraps it with six layers of gauze 2, and then opens the clip 4 provided on the rubber tube 3 , use a vacuum pump to evacuate the filter bottle 1 to a vacuum, then fill the mixed gas containing 5% oxygen through the micro sterile filter 5 until the mixed gas is full of the filter bottle 1, cultivate for 48 hours, and change the air 3 times a day;
[0029] (2) Add 50ml of sterilized 40% glycerol protective agent with equal volume concent...
Embodiment 2
[0034] The culture method of Helicobacter pylori, comprises the steps:
[0035] 1. The preparation steps of cryotube B in this embodiment 2 are the same as the preparation steps of cryotube B in embodiment 1;
[0036] 2. Enlargement and cultivation:
[0037] (1) Weigh 0.56 g of Brooke’s broth, prepare 20 ml of solution with distilled water, put it into a 50 ml suction filter bottle 1, and sterilize it with high-temperature steam to obtain liquid culture medium B, which is prepared in step 1-(2). Freezing tube B, after natural melting, pipette 2 ml of bacterial liquid to inoculate into the liquid medium B prepared in this step, add 1.5 ml of serum, plug it with a rubber stopper, wrap seven layers of gauze 2, and then place the Open the clip 4, use a vacuum pump to evacuate the filter bottle 1 to a vacuum, then fill the mixed gas containing 5% oxygen through the micro sterile filter 5 until the mixed gas is full of the filter bottle 1, cultivate for 48 hours, and change the air...
Embodiment 3
[0040] The culture method of Helicobacter pylori, comprises the steps:
[0041] 1. The preparation steps of cryotube B in this embodiment 2 are the same as the preparation steps of cryotube B in embodiment 1;
[0042] 2. Enlargement and cultivation:
[0043] (1) Weigh 0.56 g of Brooke’s Broth, prepare 20 ml of solution with distilled water, put it into a 50 ml suction filter bottle 1, and sterilize with high-temperature steam to obtain liquid medium B; take the medium in step 1-(2) Prepared frozen tube B, after natural melting, pipette 2 ml of bacterial liquid to inoculate into the liquid culture medium B prepared in this step, add 1.5 ml of serum, put on a rubber stopper, wrap eight layers of gauze 2, and then put the rubber tube Open the clamp 4 provided on 3, and use a vacuum pump to evacuate the filter bottle 1 to a vacuum, then fill the mixed gas containing 5% oxygen through the micro sterile filter 5 until the mixed gas is full of the filter bottle 1, and cultivate for ...
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