Nucleic acid extraction and amplification detection kit and detection method thereof
A detection kit and the technology of the kit are applied in the fields of biochemical equipment and methods, microbial determination/inspection, DNA preparation, etc., which can solve the problems of complex configuration work, experimental errors, and high costs, and enhance the effect of nucleic acid cleavage. , the effect of enhancing the degree of purification
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[0022] Example 1:
[0023] According to the scheme of the present invention, 40ML of nucleic acid lysis solution is prepared, including CuHCl 5.6g / 10ML, ethylenediaminetetraacetic acid 0.03g / 10ML, tris 0.06g / 10ML, NaCl 0.01g / 10ML, polyethylene glycol Octyl phenyl ether 2% (volume ratio), surfactant can be sodium lauryl sulfate, surfactant 0.075% (volume ratio), 1% (volume ratio) of phenol and isoamyl alcohol mixture, which The volume ratio of phenol to isoamyl alcohol is 20:1, and the pH value of the lysis solution is adjusted to 4.
Example Embodiment
[0024] Example 2
[0025] According to the scheme of the present invention, 40ML of nucleic acid lysis solution was prepared, including CuHCl 5.85g / 10ML, ethylenediaminetetraacetic acid 0.04g / 10ML, tris 0.07g / 10ML, NaCl 0.015g / 10ML, polyethylene glycol Octyl phenyl ether 10% (volume ratio), the surfactant can be mixed with polyvinylpyrrolidone and sodium lauroyl amide, the surfactant is 0.1% (volume ratio), 2.5% (volume ratio) of phenol, isoamyl alcohol The mixed solution, in which the volume ratio of phenol and isoamyl alcohol is 25:1, adjust the PH value of the lysate to 4.5.
Example Embodiment
[0026] Example 3
[0027] According to the scheme of the present invention, 40ML of nucleic acid lysis solution is prepared, including CuHCl 5.75g / 10ML, ethylenediaminetetraacetic acid 0.038g / 10ML, tris 0.062g / 10ML, NaCl 0.011g / 10ML, polyethylene glycol Octyl phenyl ether is 2.7% (volume ratio), the surfactant is cetylmethyltriamine bromide, the surfactant is 0.85% (volume ratio), and 2.5% (volume ratio) phenol is added to the nucleic acid lysis solution , Isoamyl alcohol mixture, in which the volume ratio of phenol to isoamyl alcohol is 22:1, and the pH value of the lysis solution is adjusted to 4.
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