Nucleic acid extraction and amplification detection kit and detection method thereof
A detection kit and the technology of the kit are applied in the fields of biochemical equipment and methods, microbial determination/inspection, DNA preparation, etc., which can solve the problems of complex configuration work, experimental errors, and high costs, and enhance the effect of nucleic acid cleavage. , the effect of enhancing the degree of purification
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0023] Prepare 40ML of nucleic acid lysate according to the scheme of the present invention, including CuHCl 5.6g / 10ML, ethylenediaminetetraacetic acid 0.03g / 10ML, trishydroxymethylaminomethane 0.06g / 10ML, NaCl 0.01g / 10ML, polyethylene glycol Octyl phenyl ether 2% (volume ratio), surfactant can be sodium lauryl sulfate, surfactant 0.075% (volume ratio), 1% (volume ratio) phenol, isoamyl alcohol mixture, wherein The volume ratio of phenol and isoamyl alcohol is 20:1, and the pH value of the lysate is adjusted to 4.
Embodiment 2
[0025] Prepare 40ML of nucleic acid lysate according to the scheme of the present invention, including CuHCl 5.85g / 10ML, ethylenediaminetetraacetic acid 0.04g / 10ML, trishydroxymethylaminomethane 0.07g / 10ML, NaCl 0.015g / 10ML, polyethylene glycol Octyl phenyl ether 10% (volume ratio), the surfactant can be mixed with polyvinylpyrrolidone and sodium lauroyl acid acid, surfactant 0.1% (volume ratio), 2.5% (volume ratio) phenol, isoamyl alcohol Mixed solution, wherein the volume ratio of phenol and isoamyl alcohol is 25:1, and the pH value of the lysate is adjusted to 4.5.
Embodiment 3
[0027]Prepare 40ML of nucleic acid lysate according to the scheme of the present invention, including CuHCl 5.75g / 10ML, ethylenediaminetetraacetic acid 0.038g / 10ML, tris 0.062g / 10ML, NaCl 0.011g / 10ML, polyethylene glycol The octyl phenyl ether is 2.7% (volume ratio), the surfactant is cetylmethyltriamine bromide, the surfactant is 0.85% (volume ratio), and 2.5% (volume ratio) of phenol is added to the nucleic acid lysate 1. Isoamyl alcohol mixed solution, wherein the volume ratio of phenol and isoamyl alcohol is 22:1, and the pH value of the lysate is adjusted to 4.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com