A preparation method of nucleoporin grafted homoporous polycarbonate biomimetic protein transport membrane
A polycarbonate and nucleoporin technology, which is applied in chemical instruments and methods, membrane technology, semi-permeable membrane separation, etc., can solve the problems of easy mass transfer restrictions, difficult to achieve industrial production and application, and expensive processing capacity of equipment.
Active Publication Date: 2021-03-09
TIANJIN POLYTECHNIC UNIV
View PDF9 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
However, the equipment required by these technologies is expensive and the processing capacity is small, and it is difficult to realize industrial production applications
In recent years, affinity chromatography has been widely used in the separation and purification of biological macromolecules such as proteins, nucleic acids, and polypeptides due to its good separation performance. This method is based on the interaction between ligands and target biomolecules, such as antibodies and antigens, sugars It is one of the most effective means in the separation mechanism of macromolecules to achieve the purpose of separation with lectins, enzymes and substrates. However, its operation is easily limited by mass transfer and the processing capacity is small.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0009] (1) Potassium persulfate is used to oxidize the polycarbonate homoporous membrane, potassium persulfate is made into an aqueous solution with a mass concentration of 20%, the polycarbonate homoporous membrane is soaked in potassium persulfate solution and reacted at 80° C. for 5 hours, Promptly get the polycarbonate membrane of surface hydroxylation; (2) configuration mass fraction is the acetonitrile solution of 5% toluene 2,6-diisocyanate, the polycarbonate membrane of surface hydroxylation is soaked in toluene 2,6-diisocyanate In the solution, react at 60 ° C for 10 minutes to convert the surface hydroxyl group into an isocyanate-NCO group; (3) configure an acetonitrile solution with a mass fraction of 5% m-phenylenediamine, and soak the film treated with diisocyanate on the surface In the phenylenediamine solution, react at 60°C for 10 minutes to aminate the membrane surface; (4) add bifunctional protein cross-linking agent 4-(p-maleimide phenyl) butyric acid succini...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
| Property | Measurement | Unit |
|---|---|---|
| Average pore size | aaaaa | aaaaa |
Login to View More
Abstract
The invention discloses a preparation method of nucleoporin grafted homoporous polycarbonate biomimetic protein transport membrane. The method comprises the following steps: (1) using plasma irradiation or persulfide oxidation to hydroxylate the surface of the polycarbonate homoporous membrane; (2) reacting the surface hydroxylated polycarbonate membrane with diisocyanate to convert the surface terminal hydroxyl group (-NCO) group; (3) the film treated with diisocyanate reacts with diamine to aminate the surface of the film; (4) the surface-aminated film is reacted with bifunctional protein cross-linking agent 4‑( The reaction of p‑maleimide phenyl) butyric acid succinimide ester makes the membrane surface have highly active maleimide; The nucleoporin is grafted onto the surface of the polycarbonate homoporous membrane by imide bonding to prepare the nucleoporin-grafted homoporous polycarbonate biomimetic membrane, which can be used to selectively transport proteins.
Description
technical field [0001] The invention relates to the field of biological separation, in particular to a preparation method of nucleoporin-grafted homoporous polycarbonate biomimetic protein transport membrane. Background technique [0002] Protein medicine is the diagnosis, prevention and treatment of diseases at the protein level. In a broad sense, protein medicine includes all products that are chemically proteins or polypeptides, such as hormones, growth factors / cytokines, proteases, receptor molecules, monoclonal antibodies and antibodies Related molecules, partial protein or peptide vaccines, etc. Compared with previous small-molecule drugs, protein drugs have the characteristics of high activity, strong specificity, low toxicity, clear biological function, and favorable clinical application. Because of its high success rate, safety and reliability, it has become an important part of pharmaceutical products. However, naturally occurring proteins, or proteins that have ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More IPC IPC(8): B01D71/50B01D67/00
CPCB01D67/0006B01D71/50
Inventor 王明霞严峰曹越超李晓丹宋健王文昭
Owner TIANJIN POLYTECHNIC UNIV