Separation and purification method of ammonia nitrogen degrading bacteria, ammonia nitrogen degrading bacteria and application
An ammonia nitrogen and microbial strain technology, applied in the field of microorganisms, can solve the problems of inability to achieve long-term treatment goals, cure the symptoms but not the root cause, and high costs, and achieve the effects of low cost, convenient operation and broad application prospects.
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Embodiment 1
[0039] The separation and purification method of ammonia nitrogen degrading bacteria specifically includes the following steps:
[0040] 1) Prepare medium for ammonia nitrogen degradation and enrichment: glucose 8g / L, ammonium sulfate 3g / L, sodium chloride 0.5g / L, dipotassium hydrogen phosphate 1.5g / L, magnesium sulfate 0.5g / L, solvent is water.
[0041] 2) Tolerate and acclimatize the black and odorous sediment samples in the above-mentioned high-concentration enrichment medium. Each generation of acclimatization takes 7 days, and a total of three generations of tolerance culture are carried out to obtain a mixed bacterial solution. The culture temperature is a constant temperature of 30°C;
[0042] 3) Prepare medium for ammonia nitrogen degradation and separation: glucose 4g / L, ammonium sulfate 0.3g / L, sodium chloride 0.5g / L, dipotassium hydrogen phosphate 0.7g / L, magnesium sulfate 0.1g / L, solvent is water;
[0043]4) Inoculate the bacterial liquid onto the above-mentioned m...
Embodiment 2
[0046] The separation and purification method of ammonia nitrogen degrading bacteria specifically includes the following steps:
[0047] 1) Preparation of medium for ammonia nitrogen degradation and enrichment: glucose 12g / L, ammonium sulfate 1g / L, sodium chloride 1.5g / L, dipotassium hydrogen phosphate 0.5g / L, magnesium sulfate 1.5g / L, solvent is water.
[0048] 2) Tolerate and acclimatize the black and odorous sediment samples in the above-mentioned high-concentration enrichment medium. Each generation of acclimatization takes 7 days, and a total of four generations of tolerance culture are carried out to obtain a mixed bacterial solution. The culture temperature is a constant temperature of 25°C;
[0049] 3) Prepare medium for ammonia nitrogen degradation and separation: glucose 7g / L, ammonium sulfate 0.1g / L, sodium chloride 1.5g / L, dipotassium hydrogen phosphate 0.3g / L, magnesium sulfate 0.3g / L, solvent is water;
[0050] 4) Inoculate the bacterial liquid onto the above-men...
Embodiment 3
[0053] The separation and purification method of ammonia nitrogen degrading bacteria specifically includes the following steps:
[0054] 1) Prepare medium for ammonia nitrogen degradation and enrichment: glucose 11g / L, ammonium sulfate 1.5g / L, sodium chloride 1.2g / L, dipotassium hydrogen phosphate 1.2g / L, magnesium sulfate 0.7g / L, solvent is water .
[0055] 2) Tolerate and acclimatize the black and odorous sediment samples in the above-mentioned high-concentration enrichment medium. Each generation of acclimatization takes 7 days, and a total of four generations of tolerance culture are carried out to obtain a mixed bacterial solution. The culture temperature is a constant temperature of 25°C;
[0056] 3) Prepare medium for ammonia nitrogen degradation and separation: glucose 5g / L, ammonium sulfate 0.2g / L, sodium chloride 1.2g / L, dipotassium hydrogen phosphate 0.6g / L, magnesium sulfate 0.2g / L, solvent is water;
[0057] 4) Inoculate the bacterial liquid onto the above-mentio...
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