Floatable and degradable microbial carrier as well as preparation method and application thereof

An easily degradable, carrier technology that can be used in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., and can solve problems such as low mechanical strength and short service life.

Active Publication Date: 2019-03-01
SHANDONG JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if the natural organic polymer carrier does not undergo other treatments, it has low mechanical strength and is easily decomposed by microorganisms, resulting in short service life and other shortcomings.

Method used

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  • Floatable and degradable microbial carrier as well as preparation method and application thereof
  • Floatable and degradable microbial carrier as well as preparation method and application thereof
  • Floatable and degradable microbial carrier as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Example 1. Preparation of a floating and easily degradable macroporous immobilized material for embedding bacterial strains

[0036] 1) According to figure 1 According to the procedure, take 2.0g of sodium alginate (SA), add 100mL of water, stir to fully dissolve, add 4mL of n-propanol to the solution as a free radical scavenger, so as to reduce the number of free radicals in the aqueous solution system, and then add 0.21g of strong oxidant Sodium periodate was placed in a temperature-controlled shaker protected from light, and oxidized at 4°C for 2 hours to obtain an activated intermediate containing an aldehyde group.

[0037]2) Add 1 mL of ethylene glycol to the reaction solution as a termination reaction reagent, stir for 15 minutes, ethylene glycol and excess sodium periodate are oxidized, the carbon chain of ethylene glycol is broken to form aldehyde, and periodate is reduced to iodic acid. After freeze-drying, the partial oxidation product OSA of SA was obtained...

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Abstract

The invention discloses a floatable and degradable microbial carrier as well as a preparation method and application thereof. The preparation method comprises the following steps: 1) dissolving a natural macromolecular gel carrier in water to form an aqueous solution, and sequentially adding a free radical scavenger and a strong oxidant into the aqueous solution for an oxidizing reaction to obtainreaction liquid of an activation intermediate containing aldehyde group; 2) adding an oxidation terminator into the reaction liquid of the step 1) to obtain a partial oxidation product of the naturalmacromolecular gel carrier; 3) performing reductive amination, alkyl grafting and alcohol precipitation separation on the partial oxidation product to obtain an amphiphilic product; 4) dissolving theamphiphilic product in water, and adding an organic macromolecular carrier, a cross-linking agent and petroleum degrading bacteria for a crosslinking reaction to obtain the floatable and degradable microbial carrier. The floatable and degradable carrier material embedding the petroleum degrading bacteria can be used for adsorbing and degrading petroleum so as to clear oil spill pollution.

Description

technical field [0001] The invention relates to a floating and easily degradable microbial carrier, a preparation method and an application. Background technique [0002] Biological treatment of oil pollution in water has the advantages of safety, environmental protection and reliability, but there are still many problems to be solved in practical application. The application of free degrading bacteria has antagonism with native microorganisms, and there are also disadvantages such as easy loss of bacteria and sensitivity to changes in environmental conditions. The immobilization technology of petroleum-degrading strains can initially solve this problem, and has a certain effect in overcoming the antagonism between free strains and native microorganisms, but there are still disadvantages such as low oil absorption rate, weak mechanical strength, and poor degradability of carrier materials, which limit the Large-scale promotion of biological treatment of oil spills. [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12N11/04C08B37/04C02F3/34C12R1/125C12R1/01C02F101/32
CPCC02F3/341C02F3/344C02F2101/32C08B37/0084C12N11/04C12N11/10
Inventor 张少君王明雨李德宇孟雄飞
Owner SHANDONG JIAOTONG UNIV
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