Method for degrading sodium alginate and agar composite membrane by using Zobellia galactanovorans

A technology of Flavobacterium zobeirii and galactan, which is applied in the field of membrane degradation, can solve the problems of slow degradation speed and achieve the effects of short degradation time, fast growth and avoiding environmental pollution

Active Publication Date: 2019-03-08
FUZHOU UNIV
View PDF5 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Biodegradable films are generally split and degraded in the natural environment through the action of microorganisms in soil and water or through the action of ultraviolet rays in sunlight. Although they can be degraded, the degradation rate is relatively slow, and they are not degraded by general bacteria in the air. degradation, which makes it an urge

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for degrading sodium alginate and agar composite membranes using Flavobacterium galactans-eating Flavobacterium zabelia, comprising the following steps:

[0019] 1) Mix 5 parts by weight of peptone, 1 part by weight of yeast extract, 0.1 part by weight of ferric phosphate, 30 parts by weight of NaCl, and 1000 parts by weight of distilled water. Prepare a culture solution, then culture the Flavobacterium galactans-eating Zobelia in the culture solution at a pH of 7.5 and 35°C for 3 days, then adjust its absorbance to 0.3 to obtain galactans-eating Flavobacterium Zobelia Bacillus culture medium, spare.

[0020] 2) Mix 75mL1 wt.% sodium alginate solution and 75mL1 wt.% agar solution, stir evenly at 55°C and pour it into a 190mm diameter petri dish, then put it in an oven at 50°C for 12h and take it out. Then add a mixed solution containing 0.5 wt% calcium chloride and 20vt% absolute ethanol, immerse the film in the solution, cross-link for 30 minutes, put it in a...

Embodiment 2

[0023] A method for degrading sodium alginate and agar composite membranes using Flavobacterium galactans-eating Flavobacterium zabelia, comprising the following steps:

[0024] 1) Mix 5 parts by weight of peptone, 1 part by weight of yeast extract, 0.1 part by weight of ferric phosphate, 30 parts by weight of NaCl, and 1000 parts by weight of distilled water. Prepare a culture solution, then culture Flavobacterium galactans-eating Zobelia in the culture medium at a pH of 7.5 and 35°C for 2 days, then adjust its absorbance to 0.3 to obtain galactans-eating Flavobacterium Zobelia Bacillus culture medium, spare.

[0025] 2) Stir 75mL of 1wt.% sodium alginate solution and 75mL of 1 wt.% agar solution at 55°C and pour them into a petri dish with a diameter of 190mm. A mixed solution of 0.5wt.% calcium chloride and 20vt% absolute ethanol, the solution is immersed in the film, after cross-linking for 30min, it is placed in an oven and dried to make small discs of appropriate size, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for degrading sodium alginate and agar composite membrane by using Zobellia galactanovorans. The method comprises the following steps: degrading a sodium alginate and agar composite membrane disc prepared early in a culture solution containing Zobellia galactanovorans for a period of time to obtain a degraded membrane, taking out the degraded membrane, filtering, washing, freezing-drying, weighing with an analytical balance, and comparing the degraded membrane with the membrane before degradation, wherein a result shows that the method disclosed by the inventionhas a relatively good degradation effect. The method disclosed by the invention is safe, environmentally-friendly, convenient and capable of effectively avoiding environmental pollution caused by film degradation by using a conventional chemical method, and has a very broad application prospect.

Description

technical field [0001] The invention belongs to the technical field of membrane degradation, and in particular relates to a method for degrading a composite membrane of sodium alginate and agar by using Flavobacterium galactan-eating Flavobacterium zobellia. Background technique [0002] Zobellia galactanovorans is a marine bacterium that exists widely in nature. The bacterium grows fast and is not easily inactivated, so it has a high application prospect. And it also contains a special effect of degrading agar, which adds to the potential of industrial application. [0003] Among the many factors causing environmental pollution, the public nuisance caused by plastic waste has aroused widespread concern in society. At the beginning of the 21st century, my country used a large amount of plastic packaging materials, and the annual consumption will reach 50 million tons. If 30% of them are disposable foamed plastics, then the annual waste plastics in the country will be more ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08L5/04C08L5/12C08K3/16C08J5/18
CPCC08J5/18C08J2305/04C08J2305/12C08J2405/04C08J2405/12C08K2003/162
Inventor 叶卓亮宋鑫卓淑敏肖玲玲王梦瑶
Owner FUZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap