Cellulase producing strain and application thereof
A cellulase and bacteria-producing technology, applied in the directions of enzymes, bacteria, enzymes, etc., can solve the problems that cannot be used to prepare cellulase, and peanut shells cannot be used to prepare cellulase, so as to improve the environment and reduce waste of resources. , the effect of high economic value
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0052] The preparation process of peanut shell powder: select peanut shells without mildew, wash with clean water to remove surface dirt, drain the water, dry naturally, then crush and pass through a 40-mesh sieve, take the sieved powder and boil for 20-40min, Dry and set aside.
[0053] 1) Add peanut shell powder into the enzyme-producing medium, and process 0.5g of peanut shell powder per 20ml of enzyme-producing medium, wherein the formula of the enzyme-producing medium is: every 1000ml of water includes KH 2 PO 4 2g, MgSO 4 .7H 2 O 0.5g, peptone 5g, yeast powder 5g, autoclaved, obtain the enzyme culture medium to be fermented;
[0054] 2) Insert the activated strain into the medium to be fermented for enzyme production, and culture it in a shaking flask at 150 r / min at 27° C. for 10 h, and the pH of the cultured fermentation broth is 6.0;
[0055] 3) Centrifuge the fermented liquid after cultivation, and take the supernatant as crude enzyme liquid; then measure the en...
Embodiment 2
[0057] The preparation process of peanut shell powder: select peanut shells without mildew, wash with clean water to remove surface dirt, drain the water and dry naturally, then crush and pass through a 40-mesh sieve, take the sieved powder, boil for 40 minutes, and dry spare.
[0058] 1) Add peanut shell powder to the enzyme-producing medium, and process 3.0 g of peanut shell powder per 20 ml of the enzyme-producing medium, wherein the formulation of the enzyme-producing medium is: every 1000 ml of water includes KH 2 PO 4 2g, MgSO 4 .7H 2 O 0.5g, peptone 5g, yeast powder 5g, autoclaved, obtain the enzyme culture medium to be fermented;
[0059] 2) Insert the activated strain into the medium to be fermented for enzyme production, and culture it in a shaking flask at 170 r / min at 42°C for 96 hours, and the pH of the cultured fermentation broth is 7.5;
[0060] 3) Centrifuge the fermented liquid after cultivation, and take the supernatant as crude enzyme liquid; then measu...
Embodiment 3
[0062] The preparation process of peanut shell powder: select peanut shells without mold, wash with clean water to remove surface dirt, drain the water, dry naturally, boil for 30 minutes, and dry for later use.
[0063] 1) Add peanut shell powder into the enzyme-producing medium, and process 1.75g of peanut shell powder per 20ml of enzyme-producing medium, wherein the formula of the enzyme-producing medium is: every 1000ml of water includes KH 2 PO 4 2g, MgSO 4 .7H 2 O 0.5g, peptone 5g, yeast powder 5g, autoclaved, obtain the enzyme culture medium to be fermented;
[0064] 2) Insert the activated strain into the medium to be fermented for enzyme production, and culture it in a shaking flask at 150-170 r / min at 34.9°C for 53 hours, and the pH of the cultured fermentation broth is 6.7;
[0065] 3) Centrifuge the fermented liquid after cultivation, and take the supernatant as crude enzyme liquid; then measure the enzyme activity (U) on the filter paper. The measured actua...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More