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Preparation method of a cordyceps militaris extract, and functional food

A technology for functional food and Cordyceps militaris, which is applied to the functions of food ingredients, medical preparations containing active ingredients, food ingredients, etc. The effect of separation cost and broad market development prospects

Inactive Publication Date: 2019-03-15
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in some current extraction methods, more emphasis is placed on the high extraction rate of a single component, but there is little research on the simultaneous extraction of multiple components in Cordyceps militaris

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0060] The preparation method includes:

[0061] Step S101: Provide an extract to be extracted.

[0062] The extract to be extracted is a crude product and is obtained from Cordyceps militaris. The extract includes a first target product and a second target product.

[0063] In addition, the aforementioned to-be-extracted material can be pre-made, or directly produced on site. In other words, the extract to be extracted can be directly purchased as a raw material or used as a prefabricated raw material.

[0064] Taking into account the stability of various active substances in Cordyceps militaris, as well as the difficulty and complexity of storage, the use of extracts can be selected according to actual production needs.

[0065] In the example, the extract can be obtained by extracting Cordyceps militaris.

[0066] Among them, extraction includes water extraction of Cordyceps militaris to obtain a water extract. The method for extracting Cordyceps militaris from water to obtain a wa...

Embodiment 1

[0108] (1) Extraction of the metabolites of Cordyceps militaris: 2kg of Cordyceps militaris raw material is crushed, passed through a 40-mesh sieve, then extracted with deionized water, and then the extract is filtered to obtain the Cordyceps militaris metabolite extract; the extraction condition is the ratio of material to liquid It is 1kg: 5L, temperature is 50°C, extraction time is 1, and the number of extractions is 1 time.

[0109] (2) Separation of metabolites in Cordyceps militaris:

[0110] The extract of Cordyceps militaris metabolites was applied to the MCI microporous resin column with a sample volume of 3 times the volume of the resin column; adsorbed for 30 minutes, then eluted with deionized water, and collected the eluate. The eluate was concentrated under reduced pressure with a rotary evaporator at a temperature of 60°C to obtain a water-extracted concentrate. Water was added as the eluent to the concentrated portion of the eluent and 3 times the amount of 95% etha...

Embodiment 2

[0116] (1) Extraction of metabolites from Cordyceps militaris: 4kg of Cordyceps militaris raw material is crushed, passed through a 120-mesh sieve, then extracted with deionized water, and then the extract is filtered to obtain the Cordyceps militaris metabolite extract; the extraction condition is the ratio of material to liquid It is 1kg: 50L, temperature is 85°C, extraction time is 3h, and extraction times are 3 times.

[0117] (2) Separation of metabolites in Cordyceps militaris:

[0118] The extract of Cordyceps militaris metabolites was applied to the MCI microporous resin column, and the amount of sample loaded was 6 times the volume of the resin column; adsorbed for 30-120 minutes, then eluted with deionized water, and collected the eluate. Concentrate the eluent under reduced pressure with a rotary evaporator at a temperature of 60°C to obtain a water-extracted concentrate. Add water as the eluent concentration to 3 times the amount of 95% ethanol, precipitate for 48 hours...

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Abstract

The invention relates to a preparation method of a cordyceps militaris extract, and a functional food, and belongs to the field of agricultural product processing. The preparation method comprises: providing a substance to be extracted; conducting water extraction on cordyceps militaris to obtain an aqueous extract of the substance to be extracted; performing a first elution operation on the aqueous extract by using microporous resin, wherein the first elution operation comprises conducting water washing, collecting a first eluate, and subjecting the first eluate to alcohol precipitation to obtain a first target substance which is precipitated as a precipitate; and performing a second elution operation after the first elution operation, wherein the second elution operation comprises performing alcohol washing, collecting a second eluate containing a second target substance, and performing a separation operation on the second eluate by liquid chromatography to obtain a second target substance. The above extraction method can achieve a more complete extraction of metabolites in the cordyceps militaris.

Description

Technical field [0001] The present invention relates to the field of agricultural product processing, in particular to a preparation method of Cordyceps militaris extract and functional food. Background technique [0002] Cordyceps militaris L. Link is also known as pupa, cordyceps, and cordyceps. It is of the same genus and different species as Cordyceps sinensis, which is a kind of medicinal and food fungus with rich nutritional value and medicinal value. Modern pharmacological studies have confirmed that Cordyceps militaris has various pharmacological activities such as antibacterial, anti-inflammatory, anti-tumor, anti-fatigue, anti-diabetic and improving immunity. Its medicinal and edible value is comparable to that of Cordyceps sinensis, so artificially cultivated Cordyceps militaris is commonly used instead of natural Cordyceps sinensis. [0003] Cordyceps militaris contains many biologically active ingredients such as adenosine, cordycepin, cordycepic acid, and polysaccha...

Claims

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Application Information

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IPC IPC(8): A23L31/00A23L33/125A23L5/10A61K36/068
CPCA23V2002/00A61K36/068A61K2236/331A61K2236/39A61K2236/53A61K2236/55A23L5/10A23L31/00A23L33/125A23V2200/30A23V2250/208A23V2300/14
Inventor 于瑞涛陶燕铎邵赟梅丽娟江磊李世华杨小兵文怀秀刘增根
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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