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Preparation method of EGCG liposome gel and EGCG liposome gel

A liposome gel, liposome technology, applied in skin care preparations, pharmaceutical formulations, cosmetic preparations, etc., can solve problems such as large membrane fluidity, drug leakage, etc., and achieve improved retention rate and good biological phase. Capacitive, good sustained release effect

Active Publication Date: 2019-03-15
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Among them, liposomes are mainly composed of non-ionic surfactants. Compared with liposomes mainly composed of phospholipids, liposomes have lower cost and better oxidation stability. However, this type of liposomes Due to the bilayer structure of the carrier, the membrane fluidity is relatively large, and it is easy to cause leakage of the drug. How to provide a preparation method that is stable, has a high retention rate, and can obtain a good EGCG slow-release effect remains to be solved in this area. technical problem

Method used

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  • Preparation method of EGCG liposome gel and EGCG liposome gel
  • Preparation method of EGCG liposome gel and EGCG liposome gel
  • Preparation method of EGCG liposome gel and EGCG liposome gel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of EGCG liposomes:

[0033] Weigh 4 portions of 170mg Tween 60 and 25mg cholesterol, add them to 10mL centrifuge tubes, add 1mL ethanol each, dissolve in a water bath at 60°C, then add 20mg of EGCG powder and vortex to fully dissolve, and dissolve in a water bath at 60°C Afterwards, a mixed solution is obtained. Use a 2.5mL syringe to slowly inject the 4 groups of mixed solutions into 20mL deionized water, pH 5.7PBS, pH6.8PBS, pH 7.4PBS hydration medium, use a high-speed disperser at 12000r / min, and disperse at a high speed under the condition of a 50℃ water bath 1 min, 50 ° C rotary evaporation to remove ethanol.

[0034] The encapsulation efficiency was determined by dialysis; after demulsification by mixing Triton TX-100 and the sample 1:1 at 0d and 30d respectively, it was diluted with water, and the EGCG content was determined by HPLC, and the retention rate was calculated.

[0035] The results showed that the particle size of the EGCG liposome prepar...

Embodiment 2

[0037] According to Table 1, take 3 parts of PVA / HA / EGCG liposome mixed solution in different proportions (wherein the EGCG liposome is prepared by Example 1), stir at room temperature at a speed of 600r / min for 6h, and obtain PVA / HA / EGCG liposome mixed solution. Put the PVA / HA / EGCG liposome mixed solution in a -20°C refrigerator, freeze for 10 hours, and then thaw at 25°C for 2 hours. This is a cycle, and a total of 3 cycles can be used to prepare PVA with different EGCG loads. / HA / EGCG liposome gel. According to Table 1, 3 parts of PVA / HA / EGCG mixed solutions with different ratios were weighed, and PVA / HA / EGCG gel was prepared according to the above steps.

[0038] Table 1: Formulations of gels with different EGCG liposome contents

[0039]

[0040] Note: The abbreviation of EGCG liposome suspension is EN, and the abbreviation of EGCG aqueous solution is ES, and the concentration of EGCG in EN and ES is 1 mg / mL.

[0041] Put a certain quality of the above six kinds of ...

Embodiment 3

[0045] Take by weighing 4 parts of different ratios of PVA / HA / EGCG liposome mixed solution (wherein the EGCG liposome is made by Example 1) according to Table 2, and stir at room temperature at a speed of 600r / min for 6h to obtain PVA / HA / EGCG liposome mixed solution. Put the PVA / HA / EGCG liposome mixed solution in a -20°C refrigerator, freeze for 10 hours, and then thaw at 25°C for 2 hours. This is a cycle, and a total of 3 cycles is obtained to obtain the mass of HA gel. PVA / HA / EGCG liposome gels with fractions of 0, 0.25%, 0.5%, and 0.75%, wherein the mass fraction of PVA in the gel is 3%, and the mass fraction of EGCG liposome suspension in the gel 25%. Put a certain amount of gel in a 25mL beaker, and add 5mL of ethanol / PBS solution (pH5.5, 20mM) mixture with a volume ratio of 1:4 to it, and place them at 1, 3, 5, 7, and 9 respectively. , 13, and 24 hours later, take out all the mixed solution and measure the content of EGCG, and add 5mL of fresh mixed solution to the bea...

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Abstract

The invention provides a preparation method of EGCG liposome gel and the EGCG liposome gel. According to the method, EGCG liposome with small particle size and high encapsulation efficiency is formedthrough ethanol injection and high-speed dispersion and then is loaded into gel, so as to form PVA / HA / EGCG liposome gel. The gel does not introduce chemical crosslinking agent, and is milder, greener,and good in biocompatibility. In addition, the PVA / HA / EGCG liposome gel has better slow-release effect than PVA / HA / EGCG gel. The release rate of EGCG can be controlled through control of the contentsof PVA and HA. The gel has a potential application value in terms of active matter slow release of cosmetics and drug controlled release of biological medicine.

Description

technical field [0001] The invention belongs to the technical field of cosmetics, and in particular relates to a preparation method of an EGCG liposome gel and the EGCG liposome gel. Background technique [0002] EGCG is the main component of tea polyphenols in tea. It has good antioxidant and anti-tumor effects, so it is often used in cosmetics, medicine and food. However, because EGCG is easily affected by factors such as pH and temperature, it shows poor stability. At the same time, due to the strong hydrophilic ability of EGCG, its bioavailability is low. Therefore, choosing a suitable carrier to coat EGCG can improve the stability of EGCG and broaden the application range of EGCG. At present, a large number of carriers with a bilayer structure have been used for the coating of EGCG, such as ethosomes, liposomes, and liposomes. [0003] Among them, liposomes are mainly composed of non-ionic surfactants. Compared with liposomes mainly composed of phospholipids, liposom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/81A61K8/49A61K8/14A61K8/04A61Q19/08
CPCA61K8/042A61K8/14A61K8/498A61K8/8129A61Q19/08
Inventor 杨成孙香梁蓉
Owner JIANGNAN UNIV
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