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Extraction method of shark chondroitin sulfate

A technology of chondroitin sulfate and extraction method, which is applied in the field of seafood processing technology, can solve the problems of loss of enzyme activity and increased cost, and achieve the effect of reducing cost

Pending Publication Date: 2019-03-22
福州宏东食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the loss of enzyme activity when immobilized enzymes are immobilized, the use of immobilized enzymes virtually increases the cost of production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The extraction method of the shark chondroitin sulfate of the present embodiment is characterized in that, comprises the following steps:

[0035] (1) Pretreatment: Wash the shark vertebral cartilage and carry out degreasing and pulverizing in turn to obtain the powder raw material, and add a concentration of 1% sodium hydroxide solution and a concentration of 5% sodium chloride solution to the obtained powder raw material, and hydrogen The addition of sodium oxide solution and sodium chloride solution is 2.5 times the weight of shark cartilage; extract at a temperature of 40°C for 12 hours, filter to obtain the filtrate, and then adjust the pH of the filtrate to 7.6;

[0036] (2) Enzymolysis: Add the first enzyme of 2% cartilage feed weight to the filtrate obtained in step (1), and enzymolyze for 3 hours at a pH value of 5.5 and a temperature of 40°C; then adjust the pH value to 6, At this time, a second enzyme of 1% by weight of the cartilage was added, and enzymolysi...

Embodiment 2

[0040]The extraction method of the shark chondroitin sulfate of the present embodiment is characterized in that, comprises the following steps:

[0041] (1) Pretreatment: Wash the shark vertebral cartilage and carry out degreasing and crushing in turn to obtain the powder raw material, and add a concentration of 1.5% sodium hydroxide solution and a concentration of 6% sodium chloride solution to the obtained powder raw material, and hydrogen The addition of sodium oxide solution and sodium chloride solution is 3.5 times the weight of shark cartilage; extract at a temperature of 50°C for 13 hours, filter to obtain the filtrate, and then adjust the pH of the filtrate to 7.9;

[0042] (2) Enzymolysis: Add the first enzyme of 3% cartilage feed weight to the filtrate obtained in step (1), and enzymolyze for 4 hours at a pH value of 6.5 and a temperature of 60°C; then adjust the pH value to 6.3, At this time, add a second enzyme of 2% by weight of the cartilage, and enzymolyze it fo...

Embodiment 3

[0046] The extraction method of the shark chondroitin sulfate of the present embodiment is characterized in that, comprises the following steps:

[0047] (1) Pretreatment: Wash the shark vertebral cartilage and carry out degreasing and pulverizing in turn to obtain the powder raw material. In the obtained powder raw material, add a concentration of 1.2% sodium hydroxide solution and a concentration of 5.5% sodium chloride solution, hydrogen The addition of sodium oxide solution and sodium chloride solution is 3 times the weight of shark cartilage; extract at a temperature of 45°C for 12.5 hours, filter to obtain the filtrate, and then adjust the pH of the filtrate to 7.8;

[0048] (2) Enzymolysis: Add the first enzyme of 2.5% cartilage weight to the filtrate obtained in step (1), and enzymolyze it for 3.5 hours at a pH value of 6 and a temperature of 50°C; then adjust the pH value to 6.2 , at this time, add a second enzyme of 1.5% by weight of the cartilage, and perform enzyma...

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Abstract

The invention relates to an extraction method of shark chondroitin sulfate. The method comprises the following steps: firstly, pretreating; secondly, enzymolysis: adding first enzyme of which the amount is 2 to 3 percent of the feeding weight of cartilage into filtrate obtained in the first step and carrying out enzymolysis for 3 to 4 hours under the conditions that the pH value is 5.5 to 6.5 andthe temperature is 40 to 60 DEG C; then adjusting the pH value to 6 to 6.3, adding second enzyme of which the amount is 1 to 2 percent of the feeding weight of the cartilage and carrying out enzymolysis for 1 to 2 hours under the condition of 50 to 60 DEG C, wherein the first enzyme is at least one of papain and alkaline protease; the second enzyme is at least one of trypsin and pepsin; thirdly, ultrafiltration; fourthly, post treatment. The extraction method of the shark chondroitin sulfate, disclosed by the invention, can replace an existing immobilized enzyme enzymolysis technology; the product yield of the shark chondroitin sulfate reaches 25 to 30 percent and the purity reaches 90 to 92 percent.

Description

technical field [0001] The invention relates to a seafood processing technology, in particular to a method for extracting shark chondroitin sulfate. Background technique [0002] Chondroitin sulfate is a mucopolysaccharide extracted from animal tissues, and its basic units are D-glucuronic acid and N-acetyl-D-galactosamine sulfate. Generally, chondroitin sulfate contains about 50-70 disaccharide units, with an average molecular weight of 10,000-30,000. The molecule has a large number of negative charges, and has lipid-lowering, anti-inflammatory and weak anti-coagulant and anti-thrombotic biological activities, and plays an important role in maintaining the relative stability and normal function of the cell environment. [0003] Chondroitin sulfate can promote the growth of fibers in the matrix, enhance permeability, improve blood circulation, accelerate metabolism, promote the absorption of permeate and eliminate inflammation; its polyanion has strong water retention and c...

Claims

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Application Information

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IPC IPC(8): C08B37/08
CPCC08B37/0069
Inventor 李健陈燕池梦秋陈忠杰徐李群叶表联罗联钰
Owner 福州宏东食品有限公司
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