A kind of plastic wrap with ethylene adsorption and preparation method thereof
A technology of adsorption and fresh-keeping film, applied in the field of fresh-keeping film with ethylene adsorption and its preparation, can solve the problems of unfavorable large-scale popularization and use, high cost of physical methods, toxic and side effects, etc., and achieves good use prospects and long acting time. , the effect of small dosage
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Embodiment 1
[0021] (1) Styrene (St), hydroxyethyl methacrylate (HEMA) perbasic alumina columns were used to remove the polymerization inhibitor in the monomer in advance. Add 5mL St, 170μL HEMA, 10mL tetrahydrofuran under nitrogen protection, react at 70°C overnight, precipitate under ice-bath n-hexane, filter and dry to obtain styrene hydroxyethyl methacrylate copolymer. Take 1.0 g of the product, dissolve it in 10 mL of THF, add 1 mL of methacrylic anhydride and 1 mL of triethylamine, react at room temperature overnight, precipitate in ice-bathed n-hexane, filter and dry to obtain a membrane material.
[0022] (2) Cultivate the mycobacterial bacteria liquid to OD600=1, take 50 mL of the bacterial liquid, centrifuge at 4000 rpm for 15 minutes, wash the bacteria sludge with phosphate buffer, and centrifuge again to get the bacteria sludge, repeat this 3 times, and finally record Weight 0.60g. Weigh 1.00g of skimmed milk powder, dissolve it with 10 mL of deionized water to obtain a 10% sk...
Embodiment 2
[0026] (1) Styrene (St), hydroxyethyl methacrylate (HEMA) perbasic alumina columns were used to remove the polymerization inhibitor in the monomer in advance. Add 5mL St, 170μL HEMA, 10mL tetrahydrofuran under nitrogen protection, react at 70°C overnight, precipitate under ice-bath n-hexane, filter and dry to obtain styrene hydroxyethyl methacrylate copolymer. Take 1.0 g of the product, dissolve it in 10 mL of THF, add 1 mL of methacrylic anhydride and 1 mL of triethylamine, react at room temperature overnight, precipitate in ice-bathed n-hexane, filter and dry to obtain a membrane material.
[0027] (2) Cultivate the mycobacterial bacteria liquid to OD600=1, take 50 mL of the bacterial liquid, centrifuge at 4800 rpm for 15 minutes, wash the bacteria sludge with phosphate buffer, and centrifuge again to get the bacteria sludge, repeat this 3 times, and finally record Weight 0.60g. Weigh 1.00g of skimmed milk powder, dissolve it with 10 mL of deionized water to obtain a 10% sk...
Embodiment 3
[0031] (1) Styrene (St), hydroxyethyl methacrylate (HEMA) perbasic alumina columns were used to remove the polymerization inhibitor in the monomer in advance. Add 5mL St, 170μL HEMA, 10mL tetrahydrofuran under nitrogen protection, react at 70°C overnight, precipitate under ice-bath n-hexane, filter and dry to obtain styrene hydroxyethyl methacrylate copolymer. Take 1.0 g of the product, dissolve it in 10 mL of THF, add 1 mL of methacrylic anhydride and 1 mL of triethylamine, react at room temperature overnight, precipitate in ice-bathed n-hexane, filter and dry to obtain a membrane material.
[0032] (2) Cultivate the mycobacterial bacteria liquid to OD600=1, take 50 mL of the bacterial liquid, centrifuge at 4500 rpm for 15 minutes, wash the bacteria sludge with phosphate buffer, and centrifuge again to get the bacteria sludge, repeat this 3 times, and finally record Weight 0.60g. Weigh 1.00g of skimmed milk powder, dissolve it with 10 mL of deionized water to obtain a 10% sk...
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