Method for establishing HPLC (High Performance Liquid Chromatography) fingerprints of stiff silkworms

A technique of fingerprinting and establishing methods, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of ensuring stability
CN109521132AInactive Publication Date: 2019-03-26SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
Publication Date
2019-03-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for establishing HPLC (High Performance Liquid Chromatography) fingerprints of stiff silkworms. The method comprises the following steps: weighing stiff silkworm powder, performing ultrasonic extraction with an 80% (V / V) ethanol solution according to a solid-liquid ratio of 1:20, filtering, evaporating to dryness, fixing the volume with methanol, and filtering by amicrofiltration membrane so as to obtain a test solution; preparing a reference solution; sucking 10uL of the test solution or mixed reference solution, injecting into a high performance liquid chromatograph to detect, and recording the fingerprints within 60 minutes; determining HPLC fingerprints of multiple batches of stiff silkworm medicinal materials, and analyzing by a similarity evaluationsystem, thereby obtaining the HPLC characteristic fingerprints of the traditional Chinese medicinal material stiff silkworms. The HPLC reference fingerprints of the stiff silkworms established by highperformance liquid chromatography have excellent precision, reproducibility and stability, and can achieve effects of comprehensively reflecting the types and quantities of the chemical components contained in the stiff silkworms and performing overall description and evaluation on the drug quality.
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Description

technical field

[0001] The invention belongs to the field of instrument analysis, and in particular relates to a method for establishing a silkworm HPLC fingerprint. Background technique

[0002] Bombyx mori is the dead body of Bombyx mori L. 4th to 5th instar larvae infected (or artificially inoculated) with Beauveria bassiana (Bals.) Vuillant.

[0003] Bombyx mori has a long history of medicinal use. In the traditional medical books of China, Korea and Japan, silkworm has the effects of relieving wind and spasm, dispelling wind and relieving pain, resolving phlegm and dispelling stagnation. tuyere Treatment of diseases such as wind-heat headache, red eyes and sore throat, rubella itching, and mumps. Modern pharmacological experiments have confirmed that the water extract or alcohol extract of Bombyx mori has various pharmacological activities such as anticonvulsant, antiepileptic, anticoagulant, hypoglycemic, antioxidative, antitumor, etc. Prospects.

[0004] At pres...

Claims

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