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Novel temperature-resisting nanometer medicine-carried system

A nano-drug loading and temperature-resistant technology, which is applied in the direction of microcapsules, anti-tumor drugs, drug combinations, etc., can solve the problems of not being able to obtain a large amount at one time, heat intolerance, and high cost, and achieve a mature extraction method and low cost , improve the release effect

Inactive Publication Date: 2019-03-29
天津善通医疗技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the large particle size of cell-derived vesicles (400-1000nm), they are unstable in vitro and are intolerant to heat; at the same time, some tumor cell-derived vesicles are used as drug carriers, which have the potential to induce tumor formation in vivo. Risk; cell-derived vesicles require high preparation and high cost, and cannot be obtained in large quantities at one time

Method used

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  • Novel temperature-resisting nanometer medicine-carried system
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  • Novel temperature-resisting nanometer medicine-carried system

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Example 1: Preparation of lipid microbubbles in milk: the milk is divided into centrifuge tubes, and the above lipid microbubbles are collected by centrifugation, including: first centrifuging at 4 degrees for 30 minutes with a centrifugal force of 13000g, Remove fat globules, casein and debris, centrifuge the obtained supernatant at 20,000 g for 1 hour, and collect the obtained precipitate as lipid microvesicles.

Embodiment 2

[0030] Example 2: Preparation of lipid microbubbles in milk: filter the milk through a polypropylene filter membrane with a pore size of 10 microns. The filtered supernatant was centrifuged at a centrifugal force of 20,000 g for 1 hour, and the obtained precipitate was collected as lipid microvesicles. The centrifugation conditions can be between 0.5-2 hours, and the obtained lipid microvesicles are basically similar.

[0031] The lipid microbubble effect that embodiment 1-2 prepares is similar, and effect explanation:

[0032] 1. Observing under a transmission electron microscope after the lipid microbubbles collected are resuspended with 0.9% (g / ml) physiological saline, from figure 1 It can be seen that the particle size range of lipid microbubbles is about 150 nanometers, and the uniformity is high, forming a bubble shape.

[0033] 2. After the collected lipid microbubbles were resuspended with 0.9% (g / ml) physiological saline, the resuspended lipid microbubbles were inc...

Embodiment 3

[0036] Example 3: The temperature-resistant nano-drug delivery system has a killing effect on tumor cells.

[0037] 1. Experimental materials and reagents: H22 mouse liver cancer cell line, the chemotherapeutic drug doxorubicin was purchased commercially, and milk was purchased commercially.

[0038] 2. Experimental steps:

[0039] 1) Preparation of lipid microbubbles. The preparation method is the same as in Example 1.

[0040] 2) Incubate the prepared lipid microbubbles and doxorubicin solution (1 mg / ml) at room temperature for 1 hour, then centrifuge at 20,000 g for 1 hour, and the precipitate is the drug-loaded lipid microbubbles encapsulating doxorubicin.

[0041] 3) H22 liver cancer tumor cells were cultured in RPMI medium1640 cell culture medium, and the tumor cells were planted in a 96-well plate, 1×10 4 pcs / hole.

[0042] Incubate the obtained drug-loaded lipid microbubbles with the equivalent of doxorubicin and 0.5, 2, 4ug / ml doxorubicin with the tumor cells resp...

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Abstract

The invention belongs to the field of nanometer medicine carrying, and particurly relates to a novel temperature-resisting nanometer medicine-carried system. The novel temperature-resisting nanometermedicine-carried system comprises fat microfoams from milk. According to the temperature-resisting nanometer medicine-carried system, the fat microfoams of which the size is about 150nm extracted fromthe milk of raised cows at a lactation period are used as a carrier, and through uniting with chemotherapeutic drugs packed in the fat microfoams, the chemotherapeutics can well act on tumor cells, and besides, the toxic and side effects of the chemotherapeutics on organisms can be reduced.

Description

technical field [0001] The invention belongs to the field of nano drug loading, and in particular relates to a novel temperature-resistant nano drug loading system. Background technique [0002] Nano-drug delivery system is widely used in tumor therapy because of its EPR effect, which can improve tumor targeting, enhance the efficacy of chemotherapy drugs and reduce side effects. Nanomaterials that have been reported to encapsulate chemotherapy drugs for targeted drug delivery include: polyethylene glycol-cephalin copolymer (PEG-PE), polylactaldehyde-glycolic acid copolymer (PLGA), etc. It is used to wrap chemotherapy drugs, and can deliver chemotherapy drugs to tumor sites, improving the killing effect of chemotherapy drugs on tumor cells. The anti-tumor nano-drug delivery system needs to pass through multiple physiological barriers in the body when reaching the target site through intravenous injection. In order to break through these physiological barriers, the ideal nan...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/46A61K31/704A61P35/00
CPCA61K9/5176A61K31/704A61P35/00
Inventor 梁清乐杨益
Owner 天津善通医疗技术有限公司
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