Synthesis method and applications of organic mineralized structure based on framework nucleic acid coding

A synthesis method and nucleic acid technology, applied in the field of nano-manufacturing, can solve problems such as inability to synthesize ordered silica materials, difficult chemical synthesis, and inability to achieve 3D scale.

Active Publication Date: 2019-03-29
SHANGHAI INST OF APPLIED PHYSICS - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this complex intracellular mineralization process involves the synergy of various organic and inorganic components, which is difficult to achieve through simple chemical synthesis
People have tried to extract proteins in living organisms and regulate the ordered silica structure required for synthesis in vitro, but so far, the most successful work reported has not been able to synthesize nano-scale highly ordered silica similar to diatom shells Material
At the same time, people also try to use other non-chemical methods to build this structure, such as template-assisted nano-etching, but it can only realize the construction of ordered silica materials on the 2D scale, and cannot be realized on the 3D scale.
At the same time, this technology has high requirements on the stability of the template.

Method used

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  • Synthesis method and applications of organic mineralized structure based on framework nucleic acid coding
  • Synthesis method and applications of organic mineralized structure based on framework nucleic acid coding
  • Synthesis method and applications of organic mineralized structure based on framework nucleic acid coding

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Embodiment 1

[0054] The present invention provides a kind of synthesis method based on the organic mineralization structure of framework nucleic acid code, comprises the following steps: SS1, the DNA origami structure (DNA-diatom) that is nucleic acid framework is provided with the diatom-like shell shape of hierarchical pore structure solution. Using DNA to directly imitate the structural unit of the diatom shell, skipping the steps of transcription and translation of nucleic acid into protein and induction of mineral synthesis. The diatom shell DNA nanostructure has three levels of hole structures with different sizes, and is formed by different types of DNA structures. Specifically, the staple short chain, M13mp18 long chain and phiX 174 long chain were mixed at a ratio of 20:1:1, in 1×TAE-Mg 2+ Buffer (Tris, 40mM; acetic acid, 20mM; EDTA, 2mM; and magnesium acetate 12.5mM; pH 8.0) was annealed, and the temperature was slowly lowered from 95°C to 25°C to obtain a DNA origami structure ...

Embodiment 2

[0070] Through molecular dynamics (MD) simulation, the importance of the synthesis method provided by the invention in preparing the DOS composite structure is explored, and verified through a control experiment. Using the 198bp 3D DNA origami structure as a model for molecular dynamics simulation, a region is obtained by extending outward from the center of the DNA origami structure, and the number of silica clusters in this region is statistically analyzed. Figure 6 is the cross-sectional view of the simulated system; Figure 7 is the number of different types of silica clusters in the selected area. When the number of TMAPS in the silica clusters is greater than 3, the number of silica clusters in the selected region exceeds the average number of silica clusters in the solution. This is due to the high concentration of Mg required for the DNA origami structure 2+ to maintain its structural stability, so a single TMAPS cannot convert Mg 2+ Compete down. Only when TMAPS ...

Embodiment 3

[0080] The analysis of the growth kinetics of the triangular DNA origami-silica complex demonstrates that this method can achieve precise replication of the DNA origami structure.

[0081] TEM statistical results show that, as Figure 14 Shown is the TEM schematic diagram before and after the nucleic acid reaction of the triangular framework. Under TEM observation, its geometric shape is highly consistent. Such as Figure 15 As shown, the average side lengths of the triangular DNA origami structure and the corresponding DOS structure are 120.2±2.3nm and 122.9±4.1nm, respectively, and the side length of the latter only increases by about 2.7nm, which shows that the framework nucleic acid coding strategy has a strong influence on the DNA origami structure. Highly reducible. At the same time, the side widths W of the two are 22.6±1.5nm and 25.9±1.4nm respectively. Compared with the theoretical side width (26nm) of the triangular DNA origami structure, the side of the pure DNA o...

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Abstract

The invention relates to a synthesis method of an organic mineralized structure based on framework nucleic acid coding. The method includes following steps: synthesizing a framework nucleic acid whichstably exists in a solution containing Mg<2+> or Ca<2+>; providing a cationic cluster with a mineral element that is Si or Ca; and bonding the cationic cluster and the phosphate skeleton of the framework nucleic acid by electrostatic attraction to form a nucleic acid-inorganic composite, thus solving a synthesis problem of the organic mineralized structure. The invention further relates to the nucleic acid-inorganic composite synthesized by the method, and applications of the nucleic acid-inorganic composite in preparation of composites of the nucleic acid-inorganic composite and metal or metal oxide, preparation of silica nanopores used for sequencing, preparation of organic inorganic multi-element composite materials with stable configuration or porous materials used for membrane separation techniques and preparation of mesoporous silica for drug targeted transport.

Description

technical field [0001] The invention relates to the field of nano-manufacturing, in particular to a synthesis method and application of an organic mineralization structure encoded by a framework nucleic acid. Background technique [0002] Through the continuous exploration of biological hard tissues, it has been generally recognized that organisms can skillfully use various inorganic minerals to construct biological minerals with different functions (such as magnetic navigation, mechanical support, protection of soft tissues, etc.). The highly regulated mechanism of organisms on the composition, structure, size and shape of biominerals has produced organic / inorganic composite hard tissues with various surprisingly complex and ordered structures and special functions. For example, diatoms, a single-celled photosynthetic organism, have a layered multi-level porous structure composed of indeterminate silica in their outer shell, and have excellent mechanical properties to prote...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B33/18C01B25/32A61K47/02B01D71/02
CPCA61K47/02B01D71/02B01D71/027C01B25/325C01B33/18C01P2006/16
Inventor 樊春海颜颢刘小果靖薪薪张菲王丽华
Owner SHANGHAI INST OF APPLIED PHYSICS - CHINESE ACAD OF SCI
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