Biological culture method for producing 2-phenylethyl alcohol with high yield from saccharomyces cerevisiae

A technology of Saccharomyces cerevisiae and biological cultivation, which is applied in the fields of bioengineering and bioengineering, can solve the problems of product separation and extraction, increase production cost, etc., and achieve the effect of green and natural products, relatively low production cost and low production cost

Inactive Publication Date: 2019-03-29
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Common 2-phenylethyl alcohol yeast production strains include: Saccharomyces cerevisiae (Saccharomyces cerevisiae), Yarrowia lipolytica (Yarrowia lipolytica) etc., people such as Albertazzi use abnormal Hansenula (Hansenula anomala CBS 110) in the L containing 2g / L -Phenylalanine without other nitrogen sources in the culture medium for 24 hours to obtain 1.7g / L 2-phenylethanol; Huang et al. used fermentation Pichia fermentans L-5 to convert L-phenylalanine The 2-phenylethanol of 453mg / L can be obtained in 16 days; Stark et al. adopt Saccharomyces cerevisiae Giv 2009 to carry out two-phase fed-batch transformation, and the final 2-phenylethanol concentration can reach 12.6g / L; Though a kind of method 2-phenylethanol yield is improved greatly, it has brought a lot of difficulties to the separation and extraction of product simultaneously, has also increased production cost

Method used

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  • Biological culture method for producing 2-phenylethyl alcohol with high yield from saccharomyces cerevisiae
  • Biological culture method for producing 2-phenylethyl alcohol with high yield from saccharomyces cerevisiae
  • Biological culture method for producing 2-phenylethyl alcohol with high yield from saccharomyces cerevisiae

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Embodiment 1

[0031] A biological culture method using Saccharomyces cerevisiae to high-yield 2-phenylethanol, inoculating Saccharomyces cerevisiae isolated from a brewery into YEPD medium and successively undergoing slant culture, solid plate culture, liquid culture, strain scale-up culture and fermentation After culturing, transform L-phenylalanine to produce 2-phenylethanol by fermentation.

[0032] Further, the optimized YEPD seed medium is used for the amplified culture of the strain; the formula of the YEPD seed medium is: peptone 20g / L, yeast extract 10g / L, glucose 20g / L and magnesium sulfate 0.5g / L , configured with 1 L as a standard, and the balance is water; the pH of the YEPD liquid seed medium is 6.0.

[0033] Further, the fermentation culture uses an optimized fermentation medium; the formula of the fermentation medium is: L-phenylalanine 15g / L, yeast extract 5g / L, glucose 25g / L, magnesium sulfate 0.5g / L L and potassium dihydrogen phosphate 5g / L are configured with 1L as standar...

Embodiment 2

[0036] A biological culture method using Saccharomyces cerevisiae to high-yield 2-phenylethanol, inoculating Saccharomyces cerevisiae isolated from a brewery into YEPD medium and successively undergoing slant culture, solid plate culture, liquid culture, strain scale-up culture and fermentation After culturing, transform L-phenylalanine to produce 2-phenylethanol by fermentation.

[0037] Further, the optimized YEPD seed medium is used for the enlarged culture of the strains; the formula of the YEPD seed medium is: peptone 18g / L, yeast extract 8.5g / L, glucose 19g / L and magnesium sulfate 0.35g / L L, configured with 1 L as a standard, and the balance is water; the pH of the YEPD liquid seed medium is 6.0.

[0038] Further, the fermentation culture uses an optimized fermentation medium; the formula of the fermentation medium is: L-phenylalanine 17.3g / L, yeast extract 3.2g / L, glucose 23g / L, magnesium sulfate 0.5 g / L and potassium dihydrogen phosphate 4.2g / L, configured with 1L as ...

Embodiment 3

[0041] A biological culture method using Saccharomyces cerevisiae to high-yield 2-phenylethanol, inoculating Saccharomyces cerevisiae isolated from a brewery into YEPD medium and successively undergoing slant culture, solid plate culture, liquid culture, strain scale-up culture and fermentation After culturing, transform L-phenylalanine to produce 2-phenylethanol by fermentation.

[0042] Further, the optimized YEPD seed medium is used for the enlarged culture of the strain; the formula of the YEPD seed medium is: peptone 22g / L, yeast extract 12g / L, glucose 23g / L and magnesium sulfate 0.62g / L , configured with 1 L as a standard, and the balance is water; the pH of the YEPD liquid seed medium is 6.0.

[0043] Further, the fermentation culture uses an optimized fermentation medium; the formula of the fermentation medium is: L-phenylalanine 22.6g / L, yeast extract 6.8g / L, glucose 26g / L, magnesium sulfate 0.6 g / L and potassium dihydrogen phosphate 6.7g / L, configured with 1L as a s...

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Abstract

The invention discloses a biological culture method for producing 2-phenylethyl alcohol with high yield from saccharomyces cerevisiae. An optimized YEPD culture medium is inoculated with saccharomycescerevisiae obtained by separation from a brewery, slant culture, solid plate culture, liquid culture, strain amplification culture and fermentation culture are performed in sequence, then L-phenylalanine is converted and fermented, and 2-phenylethyl alcohol is produced. The saccharomyces cerevisiae strain is subjected to optimized culture, and the conversion ratio and yield of 2-phenylethyl alcohol produced from L-phenylalanine by fermentation are increased.

Description

technical field [0001] The invention belongs to the field of bioengineering and technology, and in particular relates to a biological culture method using Saccharomyces cerevisiae to high-yield 2-phenylethanol. Background technique [0002] 2-phenylethanol (2-Phenylethyl alcohol, 2-PE), also known as β-phenylethanol, is an aromatic alcohol with rose fragrance, which naturally exists in many flowers and plant essential oils, such as rose, hyacinth, jasmine, lily It is widely used in daily chemical and food industries, and it is the most widely used rose-flavored formula in cosmetics. As a spice additive, 2-phenylethanol is also a flavor substance in foods such as bread, cheese, biscuits, wine, etc. It can also be used in rose, caramel, honey and other fruity food flavors, various tobacco flavors, and soap flavors. Formulated, its consumption is second only to vanillin, and is the second largest spice ingredient in the world. [0003] At present, the annual output of 2-pheny...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/22C12N1/18C12R1/865
CPCC12N1/18C12P7/22
Inventor 杨欣伟陈宇娴朱涵容陈晓钰胡小曼柯崇榕黄建忠
Owner FUJIAN NORMAL UNIV
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