Unlock instant, AI-driven research and patent intelligence for your innovation.

Detection method of amylase

A detection method, amylase technology, applied in the field of inspection, can solve problems such as complicated operation and cumbersome calculation process, and achieve the effects of simple sample processing, high detection accuracy, and intuitive and easy-to-understand results

Active Publication Date: 2019-03-29
JUNLEBAO DAIRY GRP CO LTD
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The above method is complicated to operate and the calculation process is cumbersome. The present invention aims to provide a fast, simple and intuitive detection method for amylase

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection method of amylase
  • Detection method of amylase
  • Detection method of amylase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1 A kind of detection method of amylase in white granulated sugar

[0040] (1) Mix 40g of white sugar, 40g of pure water and 20g of starch paste with pH 6.6, heat it in a water bath to 95°C while stirring, and keep the temperature for 5 minutes to obtain the test solution;

[0041] (2) Put the experimental solution prepared in step (1) in a 75°C water bath for 24 hours to obtain the test solution;

[0042] (3) Shake the solution to be tested prepared in step (2), take 5 μL of the solution to be tested and 10 μL of potassium iodide-iodine solution on a glass slide, spread it evenly with a glass rod, cover it with a cover glass, and observe under a microscope Morphology of starch granules.

Embodiment 2~6

[0043] The detection method of amylase in embodiment 2~6 white granulated sugar

[0044] Examples 2 to 6 are respectively a method for detecting amylase in white granulated sugar. The operation steps are the same as in Example 1, except that the control parameters such as the amount of raw materials added, reaction temperature, reaction time and sampling volume are different. See the table below:

[0045]

Embodiment 7

[0046] Example 7 A method for detecting amylase in glucose

[0047] (1) Mix 40g of glucose, 40g of pure water and 20g of starch paste with a pH of 6.8, heat it in a water bath to 95°C while stirring, and keep the temperature for 5 minutes to obtain the experimental solution;

[0048] (2) Put the experimental solution prepared in step (1) in a 75°C water bath for 24 hours to obtain the test solution;

[0049] (3) Shake the solution to be tested prepared in step (2), take 5 μL of the solution to be tested and 10 μL of potassium iodide-iodine solution on a glass slide, spread it evenly with a glass rod, cover it with a cover glass, and observe under a microscope Morphology of starch granules.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention discloses a detection method of amylase. A principle of the detection method is as follows: after a sample to be detected and starch are mixed, the mixture is dissolved in water,the dissolved mixture is stained with a potassium iodide-iodine solution, morphology of starch granules is observed under a microscope, and morphological changes of the starch granules are used to determine whether the sample to be detected contains the amylase. If the starch granules swell significantly, become lighter in color or disappear, the sample to be detected contains the amylase. If themorphological changes of the starch granules are not shown, the sample to be detected is free of the amylase. The provided detection method is simple and convenient to operate, intuitive and easy tounderstand in results, high in detection precision, simple in sample processing and small in required sample amount.

Description

technical field [0001] The invention belongs to the technical field of inspection, in particular to a method for detecting amylase. Background technique [0002] The full name of amylase is 1,4-α-D-glucan hydrolase, which catalyzes the hydrolysis of starch and glycogen to produce glucose, maltose and dextrin containing α-1,6-glycosidic bond branch. The raw and auxiliary materials for making yogurt contain many kinds of sugars, such as white sugar, glucose, whey protein powder, fruit jam and so on. If the raw material contains amylase, it will have an important impact on the quality of yogurt. Therefore, the detection of amylase in yogurt raw materials is of great significance. [0003] At present, there are four types of methods for the determination of amylase: [0004] The first is to determine the consumption of substrate starch, including viscosity method, turbidity method and iodine-starch colorimetry, etc.; the second is the sugar production method, which measures t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/40
CPCC12Q1/40
Inventor 李建磊刘凤昝王少波张广辉岳旭良杨新尧苏海涛于文花姚欢付翠霞刘豆康志远王红叶
Owner JUNLEBAO DAIRY GRP CO LTD