A method for rapid detection of lignocellulose content in ramie leaves by near-infrared
A lignocellulosic and near-infrared technology, applied in the biological field, can solve the problems of expensive equipment, complicated operation, and inability to carry out mass production, and achieve the effect of low-cost and rapid screening technology
Active Publication Date: 2021-05-04
HUNAN AGRICULTURAL UNIV
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Problems solved by technology
Traditionally, 72% concentrated sulfuric acid method is commonly used for the determination of lignin content, oxidation method of sulfuric acid and potassium dichromate, nitric acid ethanol method, differential gravity method for determination of cellulose content, lye extraction method and 2mol / L hydrochloric acid for determination of hemicellulose content The main disadvantage of these methods is that the operation is complicated, time-consuming and cannot be carried out in large quantities.
Although the results of high performance liquid chromatography for the determination of lignin, cellulose, and hemicellulose are accurate, the equipment is expensive
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[0029] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0030] The test materials were 110 parts of ramie leaf powder, and each part was divided into two groups, one group was used for chemical composition determination, and the other group was used for near-infrared spectrum collection. The near-infrared spectrum sample set is divided into a calibration set and a test set. The calibration set is used for model building and internal cross-validation of the model, and the test set is used for external validation of t...
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The invention discloses a method for quickly detecting the lignocellulose content of ramie leaves by infrared. First, the chemical measurement method is used to measure the component contents of cellulose, hemicellulose, lignin and lignocellulose in the sample ramie leaf powder; The agricultural product quality analyzer scans the absorption spectrum data of the sample ramie leaf powder; then selects the spectral pretreatment method of cellulose, hemicellulose, lignin and lignocellulose; uses the scanned absorption spectrum data, combined with the detection value of the chemical measurement method and The spectral data obtained by the spectral preprocessing method was used to establish a spectral analysis model; other ramie leaf powders were selected to correct the spectral analysis model; The standard equation predicts the content of cellulose, hemicellulose, lignin and lignocellulose in the ramie leaves to be tested. Rapid and efficient detection of lignocellulose content in ramie leaves.
Description
technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for quickly detecting the lignocellulose content of ramie leaves by near infrared. Background technique [0002] Research at home and abroad has found that the tender stems and leaves of ramie are rich in crude protein, crude fiber, trace elements, amino acids and other nutrients, which can be used as high-quality protein feed. Simultaneously ramie is rich in lignocellulose, and the content of lignocellulose is a key indicator for evaluating feed, and feed with high lignocellulose content is not conducive to the digestion and absorption of nutrition by animals. Therefore, it is particularly important to screen out ramie leaf germplasm with low lignocellulose content and suitable for high-quality protein feed. [0003] Lignocellulose consists of lignin, cellulose, and hemicellulose. Traditionally, 72% concentrated sulfuric acid method is commonly used for the determination ...
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IPC IPC(8): G01N21/359
CPCG01N21/359
Inventor 邢虎成马玉申刘艳刘泽航揭雨成
Owner HUNAN AGRICULTURAL UNIV