A rapid and effective method for determining the total sugar content in sorbitol liquid

Abstract

The invention relates a rapid and effective method for determining total sugar content in sorbitol liquor. The method comprises the following steps: obtaining the content of maltotetraol, maltotriol and maltitol by high performance liquid chromatography; and calculating the total sugar content by a formula: maltotetraol content * a + maltotriol content * b + maltitol content * c + d = total sugarcontent of sorbitol liquid according to the data of the three contents. The invention ensures reliable result of a test for determining the total sugar content in sorbitol liquid, which not only savesreagents, but also greatly shortens the measurement time, thereby improving the detection efficiency.

Description

technical field [0001] The invention belongs to the technical field of sugar alcohol determination, in particular to a method for quickly and effectively measuring the total sugar content in sorbitol liquid. Background technique [0002] The starch milk or starch obtained from corn and other plants is hydrolyzed and refined to make glucose, hydrogenated under the action of a catalyst, and then refined and concentrated to obtain the food additive sorbitol liquid. Sorbitol liquid is a colorless and transparent viscous liquid, in which the content of sorbitol is more than 50%, and the total sugar content is less than 8%. Among them, the total sugars include reducing glucose, fructose, pentose, lactose and sucrose (1 molecule of glucose and 1 molecule of fructose after hydrolysis), maltose (after hydrolysis into 2 molecules of glucose), etc. The methods for determining the total sugar content are all based on its ability to be oxidized by various reagents. In the national sta...

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Problems solved by technology

[0004] It can be seen from the above specific operation steps that the national standard method has the following two problems: 1) The detection takes a long time; the hydrolysis of the sample liquid takes 45-50 minutes, plus post-cooling, redox sugar reaction, filtration, dissolution, titration, etc. 1 step, analyze a sorbitol liquid sample for at least 2 hours; 2) There are many steps, and the reproducibility of the test is relatively poor. The same sample has been transferred multiple times and chemically reacted multiple times, and each step will affect the accuracy of the final test result.

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