Fermented compound drink and preparation method thereof
A compound formula and beverage technology, applied to bacteria, yeast-containing food ingredients, food ingredients and other directions used in food preparation, can solve the problems of poor effect and low curative effect, achieve significant economic and social benefits, and regulate intestinal bacteria. Effects of cluster structure, fermentation conditions and process simplicity
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[0029] 1. Preparation of lactic acid bacteria seed solution
[0030] 1) Prepare seed medium materials: 20 g glucose, 10 g peptone, 10 g beef extract, 5 g yeast extract, 5 g sodium acetate, 2 g diammonium hydrogen citrate, 0.25 g manganese sulfate, 0.6 g magnesium sulfate, 2 g potassium dihydrogen phosphate, 1ml Tween 80, heat water until completely dissolved, then dilute to 1L with tap water, adjust pH 6.5-7.5, fill in 200ml bottles, add 0.7g calcium carbonate to each bottle, sterilize at 0.1 MPa 121°C for 20min, then cool for later use ;
[0031] 2) Activation culture of strains: draw single colonies of Lactobacillus casei, Lactobacillus plantarum and Lactobacillus rhamnosus on the MRS plate medium, culture them in an incubator at 37°C for 48 hours, and expand them Cultivate the required strains;
[0032] 3) Seed liquid: Use the culture medium prepared in step 1) cooled to room temperature, inoculate the three strains in equal amounts under sterile conditions, shake well an...
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