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Placenta-like chondroitin sulfate A targeted delivery system and preparation method and application thereof

A chondroitin sulfate and delivery system technology is applied in the field of placenta-like chondroitin sulfate A targeted delivery system and its preparation, which can solve the problems of no literature report on receptors and delivery systems, and achieves improved diagnosis or treatment effect and good target tropic effect

Active Publication Date: 2019-04-05
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the specific receptor of pl-CSA is unknown, and there are no literature reports on receptors and delivery systems that can specifically bind to pl-CSA.

Method used

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  • Placenta-like chondroitin sulfate A targeted delivery system and preparation method and application thereof
  • Placenta-like chondroitin sulfate A targeted delivery system and preparation method and application thereof

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0076] A preparation method of placenta-like chondroitin sulfate A targeted delivery system, comprising the following steps:

[0077](1) 0.1 g of human serum albumin and 0.2 g of glucose were dissolved in 2 mL of isotonic phosphate buffered saline (PBS) at pH=7.4, and then 10 mg of methotrexate was added thereto to obtain the first mixed Solution; wherein, the mass concentration of human serum albumin is 5%, and the mass concentration of glucose is 10%;

[0078] (2) Pass 3mL of octafluoropropane into the first mixed solution, vibrate for 2-5min, and then sonicate for 1-3min to obtain a suspension; wherein, the power of the sonication is 200W, and the frequency is 20kHz;

[0079] (3) Add 100 μL of biotin (the mass of biotin is 0.1 mg) to the suspension, stir evenly, let stand at 4° C. for 1 hour, and collect the sediment in the lower layer that settles at the bottom of the solution;

[0080] (4) Add an isotonic PBS solution of pH=7.4 to the above-mentioned sediment for washing...

Embodiment 2

[0086] A preparation method of a pl-CSA targeted delivery system, comprising the following steps:

[0087] (1) The bovine serum albumin of 0.18g and the fructose of 0.39g are dissolved in the isotonic phosphate buffer saline (PBS) of 3mL, pH=7.4, then add 36mg curcumin wherein, obtain the first mixed solution; Wherein , the concentration of bovine serum albumin is 6%, the concentration of fructose is 13%;

[0088] (2) Pass 3mL of octafluoropropane into the first mixed solution, vibrate for 2-5min, and then sonicate for 1min; obtain a suspension; wherein, the power of the sonication is 300W, and the frequency is 20kHz;

[0089] (3) Add 200 μL of biotin (the mass of biotin is 2 mg) to the suspension, stir evenly, let stand at 6° C. for 30 minutes, and collect the lower sediment that settles at the bottom of the solution;

[0090] (4) Add PBS solution to the above sediment to wash, centrifuge at 3100rpm for 4min, repeat the washing-centrifugation step 3 times to remove unbound b...

Embodiment 3

[0094] A preparation method of a pl-CSA targeted delivery system, comprising the following steps:

[0095] (1) Dissolve 0.125 g of egg albumin and 0.3 g of fructose in 2.5 mL of isotonic phosphate buffered saline (PBS) at pH=7.4, and then add 20 mg of methotrexate therein to obtain the first mixture Solution; Wherein, the concentration of bovine serum albumin is 5%, and the concentration of fructose is 12%;

[0096] (2) Add 70 mg of perfluorooctyl ammonium bromide (PFOB) to the first mixed solution, and sonicate for 1 min to obtain a suspension; wherein, the power of the sonication is 300W, and the frequency is 20kHz;

[0097] (3) Add 100 μL of biotin (the mass of biotin is 0.2 mg) to the suspension, stir evenly, let stand at 10° C. for 40 minutes, and collect the lower sediment that settles at the bottom of the solution;

[0098] (4) Add isotonic PBS solution to the above sediment to wash, centrifuge at 3500rpm for 2min, repeat the washing-centrifugation step 3 times to remo...

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Abstract

The invention provides a placenta-like chondroitin sulfate A targeted delivery system, and further provides a preparation method and application of the placenta-like chondroitin sulfate A targeted delivery system. The placenta-like chondroitin sulfate A targeted delivery system comprises a serum albumin layer and a saccharide molecule, the saccharide molecule adheres to the serum albumin layer, and the serum albumin layer is further grafted with polypeptides of targeted placenta-like chondroitin sulfate A, wherein the polypeptides are grafted with serum albumins in the serum albumin layer through biotin-avidin specific binding. The amino acid sequence of the polypeptides is selected from one or more of amino acid sequences shown in SEQ ID NO: 1-SEQ ID NO: 3. The targeted delivery system iscapable of specifically targeting tissue which is inappropriately expressed by the placenta-like chondroitin sulfate A.

Description

technical field [0001] The invention belongs to the technical field of drug carriers, in particular to a placenta-like chondroitin sulfate A targeted delivery system and its preparation method and application. Background technique [0002] Chondroitin sulfate (CS) is a class of glycosaminoglycans covalently attached to proteins to form proteoglycans. Chondroitin sulfate is widely distributed in the extracellular matrix and cell surface of animal tissues, and plays an important physiological function. Although the polysaccharide skeleton of chondroitin sulfate is simple, there are large differences in terms of the degree of sulfation, the distribution of sulfate groups and the distribution of two different isomeric uronic acids in the chain. The fine structure of chondroitin sulfate determines its functional specificity and its interaction with various protein molecules. [0003] Placenta-like chondroitin sulfate A (pl-CSA) belongs to the glycosaminoglycan family, which are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K47/26A61K31/519A61P35/00
CPCA61K9/5015A61K9/5052A61K31/519
Inventor 范秀军张保珍陈杰张键
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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