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Preparation method of liquid nisin

A nisin and liquid technology, applied in chemical instruments and methods, food preservation, food science, etc., can solve the problems of nisin instability, low extraction yield, cumbersome use, etc., to reduce energy consumption, Improve the product yield and improve the effect of the effect

Pending Publication Date: 2019-04-09
山东元泰生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the traditional nisin products are solid products with cumbersome use, low extraction yield, high energy consumption and high pollution, while liquid products are very few. The main reason is that nisin is unstable in liquid state and easy to extract. Inactivation; With the advancement of technology, this problem is gradually solved. The advantages of liquid products, such as convenient use, high extraction yield, low energy consumption and low pollution, will surely attract the attention of more and more manufacturers.

Method used

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  • Preparation method of liquid nisin
  • Preparation method of liquid nisin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1) Put the fermented nisin fermentation broth with a titer of 10000IU / mL into the foam separation tower, 3 The ventilation rate per hour is fed into compressed air with an outlet pressure of 0.2-0.4MPa for foam separation;

[0032] 2) Use a collector to collect the foam concentrate, and according to the condition of the foam in the collector, add a total amount of 0.001% to 0.005% (v / v) polyoxypropylene polyoxyethylene glyceryl ether defoamer to it for defoaming. Soaking treatment to obtain a 4-fold concentrated solution (more than 35,000 IU / mL titer);

[0033] 3) Add food-grade hydrochloric acid with a concentration of 30-32% (w / w) to the defoamed concentrated solution, and adjust its pH value to 2.0-3.0;

[0034] 4) Heat the concentrated solution after pH adjustment to 60-100°C in a water bath, and maintain it for 10-60 minutes;

[0035] 5) Filtrate with a ceramic membrane with a cut-off pore size of 50-200 nm to obtain a ceramic membrane filtrate (potency above 30,...

Embodiment 2

[0039] 1) Put the fermented nisin fermented liquid with a titer of 9000IU / mL into the foam separation tower, and 3.0m from the bottom 3 The ventilation rate per hour is fed into compressed air with an outlet pressure of 0.2-0.4MPa for foam separation;

[0040] 2) Use a collector to collect the foam concentrate, and according to the condition of the foam in the collector, add a total amount of 0.001% to 0.005% (v / v) polyoxypropylene polyoxyethylene glyceryl ether defoamer to it for defoaming. Soaking treatment to obtain a 6-fold concentrated solution (more than 50,000 IU / mL titer);

[0041] 3) Add food-grade hydrochloric acid with a concentration of 30-32% (w / w) to the defoamed concentrated solution, and adjust its pH value to 2.0-3.0;

[0042] 4) Heat the concentrated solution after pH adjustment to 60-100°C in a water bath, and maintain it for 10-60 minutes;

[0043] 5) Filtrate with a ceramic membrane with a cut-off pore size of 50-200 nm to obtain a ceramic membrane filtr...

Embodiment 3

[0047] 1) Put the fermented nisin fermentation broth with a titer of 8000IU / mL into the foam separation tower, 3 The ventilation rate per hour is fed into compressed air with an outlet pressure of 0.2-0.4MPa for foam separation;

[0048]2) Use a collector to collect the foam concentrate, and according to the condition of the foam in the collector, add a total amount of 0.001% to 0.005% (v / v) polyoxypropylene polyoxyethylene glyceryl ether defoamer to it for defoaming. Soaking treatment to obtain concentrated 8-fold concentrated solution (titer 65000IU / mL or more);

[0049] 3) Add food-grade hydrochloric acid with a concentration of 30-32% (w / w) to the defoamed concentrated solution, and adjust its pH value to 2.0-3.0;

[0050] 4) Heat the concentrated solution after pH adjustment to 60-100°C in a water bath, and maintain it for 10-60 minutes;

[0051] 5) Filtrate with a ceramic membrane with a cut-off pore size of 50-200 nm to obtain a ceramic membrane filtrate (a potency of...

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Abstract

The invention discloses a preparation method of liquid nisin, and belongs to the field of biological food additives. The preparation method comprises the following steps: carrying out foam separationon fermented nisin fermentation liquid; collecting the foam concentrated liquid, and performing defoaming treatment to obtain concentrated solution with different concentration multiples; adjusting the pH of the concentrated solution; heating the pH adjusted concentrated solution to a certain temperature for a period of time; filtering the concentrated solution through a ceramic membrane to obtainceramic membrane filtrate; and performing temperature treatment on the ceramic membrane filtrate, adding a protective agent thereto for complete dissolution, and mixing uniformly to obtain the liquidnisin. The preparation method of the liquid nisin is convenient to use, high in extraction yield, low in energy consumption and low in pollution.

Description

technical field [0001] The invention relates to the field of biological food additives, in particular to a method for preparing liquid nisin. Background technique [0002] Nisin is a polypeptide antibacterial substance extracted from the fermentation product of Lactococcus lactis. It is a world-recognized safe and natural biological food preservative and antibacterial agent. In 1928, Rogers and Whittier first discovered it, and in 1944 Mattick and Hirsch named it N_inhibitorySubstance for the first time, that is, group N antibacterial substances, referred to as Nisin. In 1951, Hirsch et al applied nisin to cheese preservation and achieved success. It was first sold commercially in 1953 by the British company Aplin and Barrett. In 1969, the FAO / WHO Joint Expert Committee on Food Additives approved nisin as a biological preservative for the food industry. In 1988, the US National Food and Drug Administration (FDA) officially approved the application of nisin in food. my co...

Claims

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Application Information

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IPC IPC(8): A23L3/3463C07K14/315
CPCA23L3/34635C07K14/315
Inventor 徐洪利李东平高祥友
Owner 山东元泰生物工程有限公司
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