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Medium for separating microorganisms in sauerkraut fermentation liquid and preparation method thereof

A technology of culture medium and fermentation broth, applied in bacteria and other directions, can solve the problems of unfavorable microbial diversity cultivation and separation, and achieve the effect of simple and quick configuration method.

Active Publication Date: 2019-04-09
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned medium belongs to the nutrient-rich medium. Due to competitive inhibition during the cultivation process, the rapid growth of dominant microorganisms inhibits the growth of a small number of microorganisms, which is not conducive to the cultivation and isolation of microbial diversity.

Method used

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  • Medium for separating microorganisms in sauerkraut fermentation liquid and preparation method thereof
  • Medium for separating microorganisms in sauerkraut fermentation liquid and preparation method thereof
  • Medium for separating microorganisms in sauerkraut fermentation liquid and preparation method thereof

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Embodiment 1

[0035] A kind of substratum that is used for the microbial separation in sauerkraut fermented liquid comprises the steps:

[0036] (1) Preparation of Northeast sauerkraut fermented liquid samples: Firstly adopt the traditional Northeast pickled cabbage pickling method and ferment for 5 days at a temperature of 5-10°C; At the positions of the middle and lower layers, take 5 mL of fermentation broth and mix them evenly; after low-temperature centrifugation, take the supernatant, dilute 20 times with ultrapure water, and filter through a 0.22 μM filter membrane to obtain a sample of kimchi fermentation broth;

[0037] (2) Determination of the C source of the microbial culture medium: For the pickle fermentation broth sample, the high-performance liquid chromatography method was used, the chromatographic column model was Pursuit5C18 (5μm, 4.6mm×250mm), and the mobile phase was 0.01M / L hydrogen phosphate Diammonium and pure methanol (9:1), the flow rate is 0.6-0.8mL / min, the column...

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Abstract

The invention relates to a medium, in particular to a medium for separating microorganisms in sauerkraut fermentation liquid and a preparation method thereof. The preparation method comprises the following steps: selecting sauerkraut fermentation liquid, determining organic acid species of the sample by adoption of high performance liquid chromatography, and then selecting sodium salts corresponding to several organic acids as sources of a medium C; detecting the types of N sources in the sample by adoption of an ion chromatography method and an indophenol blue colorimetric method; and finallydetermining the components of the medium: every 1L of the medium contains 0.1-0.5 g of magnesium sulfate, 0.5-1.0 g of dipotassium phosphate, 0.3-0.9 g of sodium malate, 0.1-0.9 g of sodium oxalate,0.1-0.7 g of sodium fumarate, 0.1-0.5 g of sodium nitrate, 0.1-0.5 g of sodium nitrite and 15-20 g of agar, wherein the pH is 6.8-7. The medium is simple and convenient in preparation method and provides a new medium for screening functional microorganisms.

Description

technical field [0001] The invention relates to a culture medium, in particular to a culture medium for separating microorganisms in sauerkraut fermentation broth and a preparation method thereof. Background technique [0002] The culture medium is a mixed nutrient for the growth, reproduction and metabolism of microorganisms. Due to the different types of microbial nutrition, experiments and research purposes, the types and raw materials of the medium are also different, but the medium generally must contain the carbon source (C source), nitrogen source (N source), and inorganic salts necessary for microorganisms. , auxin and water, etc. The conventional microbial culture medium preparation mostly uses mixed C sources, such as a variety of sugars mixed with sucrose, lactose, etc. or glucose with the simplest structure and the most easily utilized. N sources mainly choose peptone and yeast extract Other protein N sources, supplemented by adding a small amount of inorganic s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
CPCC12N1/20
Inventor 朱琳高凤魏巍
Owner JIANGSU UNIV
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