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A method for preparing gold nanoparticles using polymyxin e as a template

A polymyxin and nano-gold technology, applied in nanotechnology and other directions, can solve problems such as being difficult to apply to biological systems, and achieve the effects of simple operation, uniform particle size and good stability

Active Publication Date: 2020-04-21
河北柏默科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most polymers are toxic and difficult to apply to biological systems

Method used

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  • A method for preparing gold nanoparticles using polymyxin e as a template
  • A method for preparing gold nanoparticles using polymyxin e as a template
  • A method for preparing gold nanoparticles using polymyxin e as a template

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] S1, preparation of polymyxin E solution:

[0046] Dissolve polymyxin E in distilled water to prepare a polymyxin E solution with a concentration of 3 mg / mL;

[0047] S2, preparation of chloroauric acid solution:

[0048] With the mass ratio of distilled water and chloroauric acid 1:0.4, prepare a concentration of 0.4mg / mL chloroauric acid solution;

[0049] S3, prepare ascorbic acid solution:

[0050] With the mass ratio of distilled water and ascorbic acid 1:1, prepare ascorbic acid solution with a concentration of 1 mg / mL;

[0051] S4, preparation of gold nanoparticles:

[0052] Add 400 μL of polymyxin E solution into a 2 mL centrifuge tube, and mix the polymyxin E solution with the chloroauric acid solution evenly at a molar ratio of 1:1 between the polymyxin E solution and the chloroauric acid solution , put the mixed solution into a metal bath, add 400 μL of phosphate buffered saline solution with pH=7.4 and 400 μL of distilled water, rotate at 600 rpm, incubat...

Embodiment 2

[0058] S1, preparation of polymyxin E solution:

[0059] Dissolve polymyxin E in distilled water to prepare a polymyxin E solution with a concentration of 3 mg / mL;

[0060] S2, preparation of chloroauric acid solution:

[0061] With the mass ratio of distilled water and chloroauric acid 1:0.4, prepare a concentration of 0.4mg / mL chloroauric acid solution;

[0062] S3, prepare ascorbic acid solution:

[0063] With the mass ratio of distilled water and ascorbic acid 1:1, prepare ascorbic acid solution with a concentration of 1 mg / mL;

[0064] S4, preparation of gold nanoparticles:

[0065] Add 400 μL of polymyxin E solution into a 2 mL centrifuge tube, and mix the polymyxin E solution with the chloroauric acid solution evenly at a molar ratio of 1:1 between the polymyxin E solution and the chloroauric acid solution , put the mixed solution into a metal bath with a rotating speed of 500rpm and incubate for 60min at a temperature of 25°C, then add an equal amount of ascorbic a...

Embodiment 3

[0070] S1, preparation of polymyxin E solution:

[0071] Dissolve polymyxin E in distilled water to prepare a polymyxin E solution with a concentration of 3 mg / mL;

[0072] S2, preparation of chloroauric acid solution:

[0073] With the mass ratio of distilled water and chloroauric acid 1:0.4, prepare a concentration of 0.4mg / mL chloroauric acid solution;

[0074] S3, prepare ascorbic acid solution:

[0075] With the mass ratio of distilled water and ascorbic acid 1:1, prepare ascorbic acid solution with a concentration of 1 mg / mL;

[0076] S4, preparation of gold nanoparticles:

[0077] Add 400 μL of polymyxin E solution into a 2 mL centrifuge tube, and mix the polymyxin E solution with the chloroauric acid solution evenly at a molar ratio of 1:1 between the polymyxin E solution and the chloroauric acid solution , put the mixed solution into a metal bath, add 800 μL of phosphate buffered saline solution with pH = 7.4, incubate at 25°C for 60 minutes at a rotation speed of...

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Abstract

The invention relates to a method for preparing nanogold particles by taking polymyxin E as a template. Firstly, a polymyxin E solution is prepared, partial gold particles are reduced by utilizing thereducibility of the polymyxin E to be served as a seed crystal; and then, an ascorbic acid is added for reduction so as to obtain the nanogold particles with the photo-thermal effect. The method comprises the following specific steps that 0.1-3 mg / mL of the polymyxin E solution is prepared, 0.01-10 mg / mL of a chloroauric acid solution is prepared, and 0.1-10 mg / mL of the ascorbic acid solution is prepared; according to the molar ratio that the polymyxin E solution to the chloroauric acid solution is 1: 0.1-10, the polymyxin E solution and the chloroauric acid solution are uniformly mixed; the mixed solution is placed into a metal bath, a phosphoric acid buffer salt solution with the pH value of 7.4 and distilled water are added, and incubation is carried out; then, the equivalent ascorbic acid solution is added step by step, wherein the interval time is 1-10 minutes every time; and after the color of the solution is changed from a light yellow to a purple blue, the nanogold particlesare obtained. The prepared nanogold particles have photo-thermal conversion performance, are uniform in particle size and good in stability.

Description

technical field [0001] The invention belongs to the technical field of metal materials, and relates to a method for preparing nano-gold particles by using polymyxin E as a template. Background technique [0002] Metal nanoparticles have broad application prospects in many fields such as molecular devices, chemical / biological sensors, catalysis, and optoelectronic materials. The preparation of metal nanoparticles is generally based on the sol-gel method, that is, the metal salt of the reducing agent is used, and then sintered to obtain nanoparticles. The nanoparticles obtained by this method have a large particle size and a wide particle size distribution, and it is difficult to Solve the reunion problem. [0003] In recent years, photothermal therapy for cancer has become a research hotspot by utilizing the unique optical properties of noble metal nanoparticles. Noble metal nanoparticles have the ability of plasmon resonance, which absorbs light energy and converts it into...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B22F9/24B82Y40/00
CPCB22F9/24B82Y40/00
Inventor 王龙刚于淑倩赵禹王静崔艳帅
Owner 河北柏默科技有限公司