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A bacterium of the genus Zorbia and its application in improving plant stress resistance

A technology of Zurbacillus and Bacillus, applied in the field of microorganisms, can solve the problems of reduced activity of PEP carboxylase and RuBP carboxylase, enhanced photorespiration, etc.

Active Publication Date: 2021-08-03
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Too much salt will also reduce the activity of PEP carboxylase and RuBP carboxylase, and strengthen photorespiration.

Method used

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  • A bacterium of the genus Zorbia and its application in improving plant stress resistance
  • A bacterium of the genus Zorbia and its application in improving plant stress resistance
  • A bacterium of the genus Zorbia and its application in improving plant stress resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] Example 1. Isolation, Identification and Preservation of Zobellella sp. DQSA1 CCTCC NO: M 2018587

[0091] 1. Isolation of bacterial DQSA1

[0092] 1. Add 10g of saline-alkali soil sample (collected from the saline-alkali grassland in Longfeng District, Daqing City, Heilongjiang Province, China) to 90mL of sterile water, shake at 28°C for 20min, let it stand for 10min, then take 1mL of the supernatant, add 9mL of Mix thoroughly in a sterile test tube in bacterial water (at this time, the dilution is recorded as 10 -1 ), then draw 1mL from this test tube and add it to another sterile test tube filled with 9mL sterile water and mix evenly, and so on to make 10 -2 、10 -3 Bacterial suspensions at different dilutions.

[0093] 2. After completing step 1, dilute 0.2mL to 10 -3 The bacterial suspension was uniformly spread on the improved S-G solid plate, and incubated at a constant temperature of 28°C for 7 days.

[0094] 3. After completing step 2, pick a single colony ...

Embodiment 2

[0118] Embodiment 2, Zorbeella genus DQSA1 can promote the rooting of mung bean cuttings

[0119] 1. Obtaining mung bean cuttings

[0120] (1) Select plump mung bean seeds and wash them with water for 2 hours.

[0121] (2) After completing the step (1), the mung bean seeds were taken, sterilized with 0.1% (v / v) sodium hypochlorite aqueous solution for 30 minutes, and then rinsed with sterile water for 10 minutes.

[0122] (3) After completing step (2), soak the mung bean seeds in sterile water for 12 hours, then sow them in sterile vermiculite, cultivate them in the dark at 27±2°C for 48 hours, and then cultivate them alternately in light and dark at 27±2°C 3d, get mung bean seedlings.

[0123] (4) After completing step (3), select mung bean seedlings whose growth, cotyledon size, and hypocotyl thickness are basically the same, and cut off the hypocotyl and root system below the cotyledon 4cm to obtain mung bean cuttings.

[0124] 2. Preparation of DQSA1 bacterial agent

...

Embodiment 3

[0134] Embodiment 3, the ability of saline-alkali stress resistance of corn and mung bean to be improved by Zorbeia genus DQSA1

[0135] The saline-alkali soil was collected in the saline-alkali grassland of Daqing City, Heilongjiang Province, China, with a pH value of 8.34, an alkalization degree of 34.28%, and a soluble salt content of 0.77%. The non-saline-alkali soil was collected from farmland in Datong District, Daqing City, Heilongjiang Province, China, with a pH value of 6.56, an alkalization degree of 7.60%, and a soluble salt content of 0.10%.

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Abstract

The invention discloses a bacterium of the genus Zorbella and its application in improving the stress resistance of plants. The bacterium of the genus Zobellella is specifically Zobellella sp. DQSA1, and its preservation number in the China Center for Type Culture Collection is CCTCC NO: M 2018587. Experiments have shown that applying Zobellella sp. DQSA1 CCTCCNO: M 2018587 to the root system of corn or mung bean can promote growth and improve resistance to saline-alkali stress, specifically expressed as plant height, fresh weight, dry weight, chlorophyll , soluble sugar content, soluble protein content and the increase of superoxide dismutase activity. The invention has important application value.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a bacterium of the genus Zorbeia and its application in improving the stress resistance of plants. Background technique [0002] In recent years, with the increase of the world's population and the reduction of arable land, some saline-alkali soils have been developed and utilized, and the saline-alkali soil has a tendency to expand. Soil salinization seriously restricts the development of global agriculture and animal husbandry, especially in arid and semi-arid areas, where saline-alkali hazards and drought stress overlap each other, seriously threatening the growth of plants. How to improve plant salt-alkali tolerance and promote plant growth, It is an urgent problem to be solved in salinized areas. [0003] The harm of saline-alkali stress to plants is mainly manifested in the following three points: (1) Physiological drought: there are too many soluble salts in the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08A01N63/20A01P21/00C12R1/01
CPCA01N63/00C05F11/08C12N1/20C12N1/205C12R2001/01
Inventor 殷奎德向君亮刘权张兴梅申永瑞王佳琪
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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