Screening method for spring-rape high-photosynthetic-efficiency germplasm

Abstract

The invention discloses a screening method for spring-rape high-photosynthetic-efficiency germplasm. The screening method includes the steps that the net photosynthetic rate, the stomatal conductance,the intercellular CO2 concentration, the transpiration rate, the stomata limiting value and the water use efficiency of leaves and pods are measured; photosynthetic areas of spring-rape green pod skins are measured; the photoresponse curve and the CO2 response curve are measured; 1) long petiole leaves, short petiole leaves, sessile leaves and the pod net photosynthetic rate of a to-be-selected material at the seedling stage, the bolting stage, the flowering stage and the pod stage are compared with long petiole leaves, short petiole leaves, sessile leaves and the pod net photosynthetic rateof the comparison variety 'Qing hybridization 7' at the seedling stage, the bolting stage, the flowering stage and the pod stage, then an average value is solved, and a material higher than 5% of thecomparison group is a primary high-photosynthetic-efficiency material; 2) the photoresponse curve and the CO2 response curve of the short petiole leaves at the seedling stage and the bolting stage aremeasured; light saturation points, light compensation points and the light quantum efficiency are computed through the light response curve, and the carboxylation efficiency, CO2 compensation pointsand CO2 saturation points are computed through the CO2 curve; germplasm at least having a highlight saturation point or low CO2 compensation point serves as a final high-photosynthetic-efficiency material.

Description

technical field [0001] The invention relates to the technical field of agricultural breeding, in particular to a method for screening spring rape germplasm with high light efficiency. Background technique [0002] High photoefficiency breeding is a method of screening low photorespiration type plants to breed crop varieties. The strength of photorespiration is often expressed by the level of carbon dioxide compensation point. The higher the intensity of photorespiration, the higher the carbon dioxide compensation point, the lower the intensity of photosynthesis, and the lower the photosynthetic efficiency; otherwise, the higher the photosynthetic efficiency. Low-light efficiency plants can be transformed into high-light-efficiency plants by hybridization, radiation induction, etc.; or individual plants or lines with low or low compensation points can be selected from low-light-efficiency plants to breed new varieties with high light-efficiency. [0003] Photosynthesis is t...

AI Technical Summary

Problems solved by technology

It is becoming more and more difficult to greatly increase the yield level of rapeseed varieties simply by using heterosis; various ways to increase crop yield in production have played a role, but this yield "bottleneck" still exists

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