Bioactive agent capable of degrading soil pesticide residues and preparation method and application thereof
A technology for bioactive agents and pesticide residues, applied in biochemical equipment and methods, methods based on microorganisms, fertilizers made from biological wastes, etc. No toxic side effects, stable structure, and the effect of promoting plant growth
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[0032] A preparation method of a bioactive agent capable of degrading soil pesticide residues, the specific steps are as follows:
[0033] The first step, fermentation, separately ferment the bacterial agents containing Bacillus amyloliquefaciens and Bacillus subtilis, and the medium is one or more of beef extract peptone medium, potato medium, and LB medium;
[0034] The second step is to prepare the composite bacterial fermentation product, filter the fermentation product of a single strain in the first step, and dry it, and mix the above-mentioned fermentation products to prepare a mixture containing Bacillus amyloliquefaciens ≥ 200 million / g, Bacillus subtilis ≥ 200 million / g;
[0035] The third step is to mix and weigh 10-15 parts of compound bacteria fermentation product, 15-20 parts of compound amino acid, 20-35 parts of humic acid, 20-30 parts of volcanic ash, and 25-35 parts of cassava wine tank;
[0036] Stir the amino acid, humic acid, volcanic ash and cassava dist...
Embodiment 1
[0043]Embodiment 1 is a bioactive agent and preparation method that can degrade soil pesticide residues, specifically as follows:
[0044] The bioactive agent includes the following components: 8 parts of amino acids, 30 parts of humic acid, 25 parts of volcanic ash; 35 parts of cassava distiller's grains, and the above ratio is based on mass ratio, and 2 parts of compound microbial fermentation products are added.
[0045] (1) Separately ferment the bacterial agents of Bacillus amyloliquefaciens, Bacillus subtilis and Pseudomonas, respectively, to obtain the fermentation broths of various bacteria, and use beef extract peptone medium, potato One or more of culture medium and LB medium. The fermentation condition of Bacillus amyloliquefaciens is 4 days at 25°C; the fermentation condition of Bacillus subtilis is 5 days at 25°C.
[0046] (2) After filtering, stirring, and drying the fermentation products of the above three single strains, the fermentation products were uniforml...
Embodiment 2
[0051] Embodiment 2 is a bioactive agent and preparation method that can degrade soil pesticide residues, specifically as follows:
[0052] The bioactive agent includes the following components: 7 parts of amino acids, 35 parts of humic acid, 30 parts of volcanic ash; 25 parts of cassava distiller's grains, and the above ratio is based on mass ratio, and 3 parts of compound microbial fermentation products are added.
[0053] (1) After the bacteria agents of Bacillus amyloliquefaciens, Bacillus subtilis and Pseudomonas were separately fermented, the fermentation broths of various bacteria were respectively obtained (fermentation conditions were the same as in Example 1).
[0054] (2) After filtering, stirring, and drying the above-mentioned three kinds of single strain fermentation products, according to the formula of 1 part of Bacillus amyloliquefaciens fermentation product, 1 part of Bacillus subtilis fermentation product and 2 parts of Pseudomonas fermentation product, The ...
PUM
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