A kind of fermentation and separation and purification method of L-leucine

Abstract

The invention provides a fermentation and separation purification method of L-leucine, and relates to the technical field of production of amino acids. According to the method, corynebacterium glutamicum and brevibacterium ammoniagenes are utilized for co-culture and production of L-leucine, and the method comprises the steps of activation culture, seed culture, fermentation culture and separationpurification of L-leucine. The fermentation culture and separation purification process for L-leucine is optimized, the yield of L-leucine is significantly increased, the generation of by-products isreduced, the fermentation time is shortened, the sugar acid conversion rate is increased, and the generation of a large amount of wastewater is avoided; a pretreatment liquid and a concentrated auxiliary are utilized, L-leucine and L-valine are effectively separated, the purity of L-leucine is significantly improved, and the method is simple and easy to control, low in cost, and has extremely-high industrial practical value.

Description

technical field [0001] The invention relates to the technical field of amino acid production, in particular to a method for fermentation and separation and purification of L-leucine. Background technique [0002] L-leucine (L-leucine) is α-amino-γ-methylpentanoic acid and α-aminoisocaproic acid, the molecular formula is C 6 h 13 o 2 N, relative molecular mass is 131.17, contains C 54.89%, N 10.67%, pure product is white crystal or crystalline powder, is a non-polar amino acid, soluble in water, insoluble in chloroform and methanol, slightly soluble in ethanol. Proust was first isolated from cheese in 1819, and later Braconnot was crystallized from the acid hydrolyzate of muscle and wool, and named leucine. Together with valine and isoleucine, it belongs to branched-chain amino acids and is one of the eight essential amino acids for human body. L-leucine has a variety of physiological functions, and has been widely used in the fields of medicine, food, chemical industry, ...

AI Technical Summary

Problems solved by technology

Among them, the ion exchange method is the most commonly used method, but when the isoelectric point difference between the mixed amino acids is small, it is not easy to separate, and there are also the following disadvantages: large salt consumption, excessive regeneration waste liquid, long operation cycle, and discharge of a large amount of amino acids containing Salt wastewater is easy to cause pipeline corrosion. When there are multiple ions in the solution, different resins need to be selected for different purpose ions, and the general applicability is poor.

But the purity of L-leucine obtained by this method is not high, and there is no disclosure of effective separation of L-leucine from other amino acids, especially L-valine

[0006] The present invention aims to solve the problems existing in the prior art, that is, low yield and purity of L-leucine, accumulation of a large number of by-products in fermentation, low sugar-acid conversion rate, complex extraction process, long time consumption, excessive regeneration waste liquid, Difficulty separating from L-valine, etc.

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