Graphene modified nano fibroin-based microbial pigment dye particle and preparation method thereof
A technology of microbial pigment and graphene modification, applied in the field of textile materials, can solve the problem of less nano-pigment, and achieve the effect of improving affinity, small molecular weight, good light resistance and heat resistance
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Embodiment 1
[0025] (1) Add Monascus fermented strains and silk protein with a mass ratio of 1:0.6 into a liquid fermentation medium containing 0.01% Tween 80, and culture on a constant temperature shaker at a frequency of 500 r / min at 28°C 3d, centrifuge to obtain a microbial pigment nanomicelle solution.
[0026] (2) Add the microbial pigment nanomicelle solution with a solid content of 1wt% at a volume ratio of 1:2 to the silk protein solution with a solid content of 3wt%, stir evenly, and freeze-thaw twice, wherein the freeze-thaw treatment The freezing temperature is -20°C, and the melting temperature is 25°C to obtain a solution of silk protein particles loaded with microbial pigments.
[0027] (3) Add graphene oxide solution dropwise to the silk protein particle solution loaded with microbial pigment, wherein graphene oxide accounts for 0.1wt% of the total system mass, after mixing evenly, according to the quality of graphene oxide and DL-cysteine The ratio is 1:0.3, add DL-cystein...
Embodiment 2
[0029] (1) Add B. trispora fermentation strain and silk protein with a mass ratio of 1:1.1 to a liquid fermentation medium containing 0.5% polyethylene glycol, and keep the temperature constant at a frequency of 800 r / min at 30°C Cultivate on a shaking table for 6 days, and centrifuge to obtain a microbial pigment nano-micelle solution.
[0030] (2) Add the microbial pigment nanomicelle solution with a solid content of 3wt% at a volume ratio of 1:4 to the silk protein solution with a solid content of 5wt%, stir evenly, and freeze-thaw 5 times, wherein the freeze-thaw treatment The freezing temperature is -20°C, and the melting temperature is 30°C to obtain a solution of silk protein particles loaded with microbial pigments.
[0031] (3) Add graphene oxide solution dropwise to the silk protein particle solution loaded with microbial pigment, wherein graphene oxide accounts for 0.3wt% of the total system mass, after mixing evenly, according to the quality of graphene oxide and D...
Embodiment 3
[0033] (1) Add the Aspergillus niger fermentation strain and silk protein with a mass ratio of 1:0.8 into the liquid fermentation medium containing 0.05% Tween 80, and culture on a constant temperature shaker at a frequency of 600r / min at 29°C 4d, centrifuging to obtain a microbial pigment nano-micelle solution.
[0034] (2) Add the microbial pigment nano-micelle solution with a solid content of 2wt% at a volume ratio of 1:2.5 to the silk protein solution with a solid content of 3.5wt%, stir evenly, and freeze-thaw 4 times, wherein The freezing temperature of the treatment is -20° C., and the melting temperature is 28° C. to obtain a solution of silk protein particles loaded with microbial pigments.
[0035] (3) Add graphene oxide solution dropwise in the silk protein particle solution loaded with microbial pigment, wherein graphene oxide accounts for 0.2wt% of the total system mass, after mixing evenly, according to the quality of graphene oxide and DL-cysteine The ratio is ...
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