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Preparation method of nano-liposome of goose blood anti-oxidation small-molecule enzymatic product

A nano-liposome and small-molecule technology, which is applied in liposome delivery, medical preparations of non-active ingredients, anti-toxins, etc., can solve problems such as low utilization rate of goose blood and environmental pollution

Pending Publication Date: 2019-05-03
YANGTZE UNIVERSITY
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  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to: provide a method that can effectively improve the utilization value and added value of goose processing by-products; solve the problems of low utilization rate and environmental pollution of existing goose blood, and further improve the added value and digestion and absorption rate of goose blood protein Preparation method of goose blood antioxidant small molecule enzymatic hydrolyzate nano-liposome with functional properties

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  • Preparation method of nano-liposome of goose blood anti-oxidation small-molecule enzymatic product
  • Preparation method of nano-liposome of goose blood anti-oxidation small-molecule enzymatic product
  • Preparation method of nano-liposome of goose blood anti-oxidation small-molecule enzymatic product

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Embodiment Construction

[0103] The fresh goose blood was centrifuged (3500r / min, 15min), and the lower layer was washed and collected with 5 times the volume of normal saline (0.9%), distilled water was added to the collected lower layer, and the volume was adjusted to the volume of the original goose blood .

[0104] The collected separation treatment liquid was subjected to ultrasonic cell disruption to release the protein in the cells, the ultrasonic power was 250w, and the time was 12min. The mixture after ultrasonic crushing was centrifuged (3500r / min, 10min) and then filtered to obtain goose hemoglobin.

[0105] Adjust the concentration of goose hemoglobin obtained by pretreatment to 6% with distilled water, and the pH value is 10.0, then preheat at 50°C for 5 minutes, then add 6000U / g alkaline protease, and enzymatically hydrolyze at 50°C in the dark for 5.0h (speed 90r / min), then inactivate the enzyme at 100°C for 10 minutes, centrifuge (3000r / min, 10min) to get the supernatant, which is the...

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Abstract

The invention relates to a preparation method of nano-liposome of a goose blood anti-oxidation small-molecule enzymatic product, and belongs to the technical field of deep processing of goose blood by-products. The method comprises the following steps: taking goose blood as a raw material, separating goose blood hemoglobin, then degrading macromolecular proteins by using an alkaline protease and an optimized process, enriching polypeptides and amino acids with antioxidant activity, simultaneously performing sequential separation to obtaining a small-molecule active substance less than 1KD, andthen embedding the small-molecule active substance by using phospholipid, cholesterol and Tween 80 so as to obtain the nano-liposome. The liposome can effectively prevent the external environment from destroying the goose blood anti-oxidation small-molecule enzymatic product, improves the stability, increases the digestibility of goose blood protein, slowly releases active ingredients in the gastrointestinal environment step by step, and gives full play to the physiological functions. The method reduces the pollution problem caused by direct discharge of goose blood to the environment, enables the goose blood to be used with high added value, and can be industrialized.

Description

technical field [0001] The invention relates to a preparation method of goose blood antioxidant small molecule enzymatic hydrolyzate nano liposome, which belongs to the technical field of goose blood by-product deep processing. Background technique [0002] Goose blood is a high-protein resource, with a protein content as high as 19%, of which hemoglobin content is about 12.99 g / 100 mL, accounting for 2 / 3 of whole blood protein. The hemoglobin contains highly active SOD, and the weight-deficient immunoglobulin IgY (△Fc) unique to waterfowl. These substances can remove free radicals in the body and enhance the immune function of the body. Preparation of antioxidant hydrolyzate from goose blood can not only rationally utilize goose blood protein resources, but also reduce environmental pollution caused by direct discharge. Using goose blood as a raw material to prepare antioxidant polypeptides can effectively increase the content of antioxidants in goose blood hydrolyzate and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/57A61K9/127A61K47/24A61K47/28A61P39/06
Inventor 孙卫青
Owner YANGTZE UNIVERSITY
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