Extraction method of tobramycin

A technology of tobramycin and extraction method, applied in the field of biomedicine, can solve the problems of long production cycle, difficult quality control of production process, complicated process, etc., to simplify production process, reduce environmental protection discharge and processing pressure, and shorten production effect of cycles
CN109705174AActive Publication Date: 2019-05-03NEW FOUNDER HLDG DEV LLC +2
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NEW FOUNDER HLDG DEV LLC
Publication Date
2019-05-03

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Abstract

The present invention provides an extraction method of tobramycin. The extraction method comprises the following steps: (1) pH of a 5'carbamoyl tobramycin fermentation filtrate is adjusted to be 7.0-7.5, a cationic resin is added for absorption, water and ammonia water are used for rinsing, and an ammonia water eluent is collected; (2) the eluent is concentrated; (3) the obtained concentrate is hydrolyzed at an alkaline condition, pH of a hydrolyzate is adjusted to be 5.0-6.0, a cationic resin is used for decolorizing, a decoloring solution is connected, pH is adjusted to be 7.0-7.5, a medium-pressure chromatography self-prepared column equipped with a cationic resin is eluted with the water and ammonia water to collect an ammonia water eluent, then a strong alkaline anion resin is used for decoloring, and a decoloring solution is connected; and (4) the obtained decoloring solution is concentrated and then decolorized with activated carbon, and then spray-drying is conducted to obtaina finished product of the tobramycin. The method simplifies production processes, uses super nanofiltration, combines the medium-pressure chromatography self-prepared column technology, shortens production cycle, is free of solvent crystals and residual solvents, and reduces environmental discharge and treatment pressure.
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Description

technical field

[0001] The invention belongs to the field of biomedicine, and in particular relates to a method for extracting tobramycin. Background technique

[0002] Tobramycin (Tobramycin) is an aminoglycoside antibiotic, which is a product obtained by fermentation of Streptomyces darkerus. At present, the fermentation process of tobramycin mainly produces 3 products of 5' carbamoyl tobramycin, apramycin and carbamycin karamycin B (kb), each accounting for about 30%, and about 10% Left and right other impurity products are hydrolyzed to produce tobramycin and kanamycin B and other by-products. These three types of substances are aminoglycoside antibiotics with very similar properties. The entire tobramycin purification process is complicated, the production cycle is long, and the quality control of the production process is difficult. Therefore, the existing tobramycin extraction method needs to be improved. Contents of the invention

[0003] The purpose of the pres...

Claims

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