Food bacterium content detection device

Abstract

The invention discloses a food bacterium content detection device. The food bacterium content detection device comprises a detection machine body, a high speed motor and an electrochemistry sensor, and also comprises a vertical guide rail, a first transversing mechanism, a probe mechanism and a loading cylinder; the vertical guide rail is arranged at the middle position of the inner side of the back of the detection machine body; the electrochemistry sensor is mounted on the vertical guide rail through an L-shaped plate, and the other side of the L-shaped plate is arranged above the electrochemistry sensor; the first transversing mechanism is mounted below the electrochemistry sensor and the probe mechanism is mounted below the transversing mechanism; and the loading cylinder is arranged below the probe mechanism, the high speed motor is arranged on the plane parallel to the loading cylinder, and the loading cylinder is connected with the high speed motor through a transmission belt. Through combination of a mechanical centrifugation manner and a DNA probe manner, the food bacterium content is thrown out under the action of centrifugal force; and through a DNA probe, detailed and accurate detection is performed. Through quality and size of obtained samples, the food bacterium content is determined, and detection results are given accurately and rapidly.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a bacteria detection device, more specifically to a food bacteria content detection device. Background technique [0002] Rapid detection of bacterial content in food is an important means to ensure food hygiene and safety. Foodborne diseases caused by pathogenic bacteria are the biggest hidden danger to food safety. The WHO report shows that there are hundreds of millions of people suffering from foodborne diseases worldwide, and about There are hundreds of millions of diarrhea cases, resulting in the death of about 3 million children under the age of 5, 70% of which are caused by food contamination by pathogenic bacteria. The detection methods for bacteria content in the existing technology include: flow cytometry, surface plasma Resonance, piezoelectric sensing, DNA probes, bioluminescence, and spectrophotometry. [0003] At present, the commonly used detection methods...

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Problems solved by technology

[0003] At present, the commonly used detection methods in food hygiene inspection include plate culture method, polymerase chain reaction (PCR) and enzyme-linked immunoassay (ELISA). , The workload is heavy, and the detection time is long, requiring more than 48 hours, which is not suitable for timely observation of a large number of samples; the PCR technology based on molecular biology is highly sensitive and relatively short in time, but the detection results are prone to false positives; EL ISA technology is also It is relatively mature, but there are many factors affecting the test results, which may easily cause distortion of the results, and the benzene reagents used for color development are likely to cause adverse effects on people and the environment

[0004] DNA probe method, also known as molecular hybridization technology, is a technology that uses the high accuracy of base complementary pairing of DNA molecules to detect specific target DNA sequences. DNA probe method has the advantages of simple production, high sensitivity and low cost. , easy to achieve miniaturization and other advantages and has been widely used, but this detection method cannot completely and thoroughly detect the bacterial content in the sample to obtain results, and it is necessary to use auxiliary tools for comprehensive detection

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