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A microfluidic chip for manipulating and pairing single particles and its application

A microfluidic chip and microparticle technology, which is applied in specific-purpose bioreactors/fermenters, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve the difficulties in the isolation and pairing process and the lack of cell types Selectivity, it is difficult to achieve multi-cell pairing, etc., to overcome the Poisson distribution, easy to operate, and cost-effective

Active Publication Date: 2020-06-09
WISDOM HEALTHY BIOTECH XIAMEN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the existing microfluidic platforms are based on the co-cultivation of cell populations, which observe the population behavior of cells, and cannot understand the interaction mode between cells at the single-cell scale
Although there are also single-cell pairing platforms based on fluid mechanics, most of the current pairing platforms are based on two cells. For multi-cell pairing with a larger number and more types, there are problems of difficult or low efficiency in the isolation and pairing process.
For example, combining microfluidic technology with light field, magnetic field, sound field, electric field, etc., although multi-cell pairing can be achieved, the cell type lacks selectivity, and the operation is cumbersome and requires high equipment.
Others such as microwell arrays and droplet systems can achieve large-scale pairing and are compatible with cells of different sizes, but due to the Poisson distribution, it is difficult to achieve a specific number and specific type of multi-cell pairing

Method used

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  • A microfluidic chip for manipulating and pairing single particles and its application
  • A microfluidic chip for manipulating and pairing single particles and its application
  • A microfluidic chip for manipulating and pairing single particles and its application

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Embodiment 1

[0064] Such as Figure 7 As shown, the chip of the present invention for manipulating and pairing single particles includes three parts: channel layer A, control layer B and slide C, wherein channel layer A is located on the uppermost layer, control layer B is located in the middle, and the slide sheet is located on the lowermost layer .

[0065] Wherein the channel layer A includes a plurality of single particle capture and transfer units, in this embodiment, these units are connected in series (such as Figure 4 and Figure 5 shown). In other embodiments, these units may also be connected in parallel or in a hybrid manner.

[0066] see Figure 1 to Figure 6 , each unit includes a capture channel 1 , a capture chamber 2 , a capture slit 3 , a transfer channel 4 , a matching chamber 5 and a matching slit 6 . exist figure 1 In , the flow direction of capture channel 1 is from left to right.

[0067] The capture chamber 2 is located in the capture flow channel 1, where a ...

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Abstract

The invention discloses a microfluidic chip for accurately controlling and pairing single particles. The chip comprises a channel layer and a control layer. The channel layer comprises a plurality ofunits for capturing and transferring single particles, and each unit consists of a capture flow channel, a capture chamber, a capture gap, a transfer flow channel, a pairing chamber and a pairing gap.The control layer is positioned below the capture flow channel and a pairing flow channel, is vertical to the capture flow channel and the pairing flow channel, and is isolated by a diaphragm. The chip can efficiently and accurately control the capture and transfer of single particles, and can realize high-throughput and high-efficiency single-particle pairing after different rounds of single-particle capture and transfer, and the number and the type of the paired particles are controllable. The chip can be widely applied to single-cell isolation and culture, single-cell heterogeneity analysis, multi-cell co-culture, multi-cell interaction, potential mechanism disclosure and the like.

Description

technical field [0001] The invention relates to a microfluidic chip for manipulating and pairing single particles, which can be applied to the isolation and culture of single cells, the analysis of single cell heterogeneity, multi-cell co-cultivation, multi-cell interaction and the revealing of potential mechanisms, etc. Background technique [0002] Due to the advantages of miniaturization, integration, high throughput, and precise control of microfluidics, microfluidic technology has been more and more widely used in the fields of physics, chemistry, biology, and engineering science. One of the important applications is to realize Precise manipulation and pairing of single particles. Taking a single cell as an example, a cell is the basic unit of the structure and function of an organism, and the function of a multicellular organism is inseparable from the interaction between single cells. Therefore, a comprehensive and accurate understanding of cells requires not only he...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/36
Inventor 杨朝勇刘卫枝李星锐黄山青朱志周雷激
Owner WISDOM HEALTHY BIOTECH XIAMEN CO LTD
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