A microfluidic chip for manipulating and pairing single particles and its application
A microfluidic chip and microparticle technology, which is applied in specific-purpose bioreactors/fermenters, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve the difficulties in the isolation and pairing process and the lack of cell types Selectivity, it is difficult to achieve multi-cell pairing, etc., to overcome the Poisson distribution, easy to operate, and cost-effective
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[0064] Such as Figure 7 As shown, the chip of the present invention for manipulating and pairing single particles includes three parts: channel layer A, control layer B and slide C, wherein channel layer A is located on the uppermost layer, control layer B is located in the middle, and the slide sheet is located on the lowermost layer .
[0065] Wherein the channel layer A includes a plurality of single particle capture and transfer units, in this embodiment, these units are connected in series (such as Figure 4 and Figure 5 shown). In other embodiments, these units may also be connected in parallel or in a hybrid manner.
[0066] see Figure 1 to Figure 6 , each unit includes a capture channel 1 , a capture chamber 2 , a capture slit 3 , a transfer channel 4 , a matching chamber 5 and a matching slit 6 . exist figure 1 In , the flow direction of capture channel 1 is from left to right.
[0067] The capture chamber 2 is located in the capture flow channel 1, where a ...
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