Magnetic bead suspension for nucleic acid purification and screening and application thereof

A suspension and magnetic bead technology, used in biochemical equipment and methods, microbial determination/inspection, DNA preparation, etc., can solve the problems of few magnetic beads, uneven purification recovery rate and quality, and achieve stable performance and cost. Low, high flexibility effects

Inactive Publication Date: 2019-05-10
BEIJING USCI MEDICAL LAB CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Domestic companies have also emerged many imitators. At present, there are many kinds of magnetic bead brands, the a

Method used

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  • Magnetic bead suspension for nucleic acid purification and screening and application thereof
  • Magnetic bead suspension for nucleic acid purification and screening and application thereof
  • Magnetic bead suspension for nucleic acid purification and screening and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0039] The preparation of embodiment 1 magnetic bead suspension (1)

[0040] This example provides a method for preparing a magnetic bead suspension. The specific formula is: carboxylated nano-magnetic beads with a particle diameter between 0.5-1.0 μm, and the final concentration in the magnetic bead suspension is 1.0 mg / mL, a final concentration of 15% (mass percentage, g / 100mL) polyethylene glycol PEG10000, a final concentration of 1mol / L sodium chloride, 5mmol / L ethylenediaminetetraacetic acid, 2mmol / L Tris-HCl , a mass volume ratio (g / 100mL) of 0.01% sodium N-lauroyl sarcosinate, a mass volume ratio of 0.5% Tween 20, and a solvent of water. After preparation, store at 2°C-8°C. In the preparation process of the magnetic bead suspension of the present invention, water, PEG, sodium chloride and other high-concentration or high-concentration components are added first, and other low-concentration components are subsequently added. There is no special requirement for the sequ...

Embodiment 2

[0041] The preparation of embodiment 2 magnetic bead suspension (2)

[0042] This embodiment provides a magnetic bead suspension, the specific formula is: the solvent is water, the carboxylated nano-magnetic beads with a particle diameter of 0.5 μm, the final concentration in the magnetic bead suspension is 5 mg / mL, and the final concentration 15% (mass percentage, g / 100mL) of polyethylene glycol PEG6000, a final concentration of 0.5mol / L of sodium chloride and a final concentration of 1mol / L of magnesium chloride, 1mmol / L of ethylenediaminetetraacetic acid, 6mmol / L of Tris-HCl, mass volume ratio of 0.05% (g / 100mL) sodium N-lauroyl sarcosinate, mass volume ratio of 0.5% 1% Tween 20. After preparation, store at 2°C-8°C.

Embodiment 3

[0043] The preparation of embodiment 3 magnetic bead suspension (3)

[0044] This embodiment provides a magnetic bead suspension, the specific formula is: the solvent is water, the carboxylated nano-magnetic beads with a particle diameter of 1 μm, the final concentration in the magnetic bead suspension is 1 mg / mL, and the final concentration is The polyethylene glycol PEG8000 of 20% (mass percentage), final concentration is the sodium chloride of 2.5mol / L and the magnesium chloride that final concentration is 1.0mol / L, the ethylenediaminetetraacetic acid of 5mmol / L, the Tris of 2mmol / L -HCl, sodium N-lauroyl sarcosinate with a mass volume ratio of 0.01%, and Tween 20 with a mass volume ratio (g / 100mL) of 0.5%. After preparation, store at 2°C-8°C.

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Abstract

The invention belongs to the technical field of magnetic bead application, and discloses a magnetic bead suspension for nucleic acid purification and screening and application thereof. The magnetic bead suspension comprises a carboxylated modified nano magnetic bead, PEG polyethylene glycol, sodium chloride and/or magnesium chloride, ethylenediaminetetraacetic acid, Tris-HCl, N-sodium lauroyl sarcosinate and Tween 20, a solvent is water, and the concentration of the nano magnetic bead is 0.1-5 mg/mL. The magnetic bead suspension can purify nucleic acid with a recovery rate of up to 96.42%, andhave a good DNA fragment screening function. The magnetic bead suspension has stable performance, is suitable for high-throughput sample operation, has low cost and high flexibility, and is suitablefor use in various workstations.

Description

technical field [0001] The invention belongs to the technical field of magnetic beads application, and in particular relates to a magnetic bead suspension used for nucleic acid purification and screening and its application. Background technique [0002] With the rapid development of genomics detection technology, especially the maturation of next-generation sequencing (NGS) technology, the need for high-throughput, rapid, high-sensitivity, and standardized experimental operations is becoming more and more urgent after it is applied to clinical diagnosis or medical research. The library construction step of NGS sequencing technology is a very time-consuming and labor-intensive task. The sample needs to be purified between the enzymatic reaction steps so that the next experiment can be carried out. [0003] In the needs of small-scale experiments or scientific research projects, nucleic acid purification can use silicon-based filter columns or filter membranes to filter the s...

Claims

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Application Information

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IPC IPC(8): C12N15/10C12Q1/6806
Inventor 孙广欣王冬王建伟伍启熹刘倩刘珂弟唐宇
Owner BEIJING USCI MEDICAL LAB CO LTD
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