A AND gate logic operation method based on linear double-stranded DNA molecular connection and its application

Abstract

The invention discloses an AND gate logic operation method based on linear double-stranded DNA molecule connection. The gene expression core assembly sequence containing a promoter, a gene coding region and a poly A signal is split into 2-3 linear double-stranded DNA molecules as the input by PCR amplification reaction and transfected into cells. When all linear double-stranded DNA molecules are simultaneously present, the linear double-stranded DNA molecules reform the complete gene expression core assembly sequence by the connection reaction in the cells and achieve gene expression to achieve the AND gate logic operation accordingly. The low noise ratio AND gate logic operation method based on the linear double-stranded DNA molecules is provided and can be widely applied to synthetic biology and biological calculation.

Description

technical field [0001] The invention relates to the field of synbiotic organisms, in particular to an AND gate logic operation method based on the connection of linear double-stranded DNA molecules. Background technique [0002] Synthetic biology (Synthetic biology) is an emerging interdisciplinary subject in the 21st century, emphasizing "design" and "redesign", and its purpose is to solve problems such as health, environmental protection and energy by artificially designing and constructing biological systems that do not exist in nature. In recent years, major breakthroughs such as the artemisinic acid microbial synthesis factory of the J.D. Keasling research group, the E. coli imaging system of the C.A. Voigt research group, and the new strains created by the J.C. Venter and G. Church research groups have demonstrated the great potential of synthetic biology to change the world. potential. Synthetic biology will have great scientific significance for human beings to unde...

AI Technical Summary

Problems solved by technology

[0005] In the prior art, the defects and deficiencies mainly include: (1) The construction strategy of the existing AND gate logic gene circuit is relatively complicated. Firstly, cells need to express various proteins or RNA molecules, and on this basis, through the relationship between these molecules and DNA molecules The interaction among them realizes the AND gate operation

Expressing protein or RNA molecules before logic operations increases the burden on cells; (2) The current AND gate logic gene circuit design strategy cannot achieve high signal-to-noise ratio, that is, in [0,0][0,1][1,0 ] to achieve a signal output close to the background, and a stronger signal output in the case of [1,1]; (3) The current AND gate logic gene circuit design strategy does not utilize the split gene expression core components (promoter- Gene coding region-poly A signal, promoter-gene-polyA signal) to achieve high signal-to-noise ratio output

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