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Application of a compound thrombus links agent in preparing anti-inflammatory medicines

A technology of compound Xueshuantong and anti-inflammatory drugs, which is applied in the direction of anti-inflammatory agents, drug combinations, antipyretics, etc., and can solve the problem of anti-inflammation of compound Xueshuantong capsules that have not been seen

Inactive Publication Date: 2019-05-21
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the anti-inflammatory aspects of Fufang Xueshuantong Capsules

Method used

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  • Application of a compound thrombus links agent in preparing anti-inflammatory medicines
  • Application of a compound thrombus links agent in preparing anti-inflammatory medicines
  • Application of a compound thrombus links agent in preparing anti-inflammatory medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1 in vivo anti-inflammatory experiment

[0019] 1. Experimental materials

[0020] 1.1 Test drug: Fufang Xueshuantong Capsules

[0021] 1.2 Control drug: heparin

[0022] 2. Experimental animals

[0023] 60 SD rats, SPF grade, male, weighing 150-200 g, were provided by the Guangdong Medical Experimental Animal Center, and the experimental animal quality certificate number: SCXK (Guangdong) 2012-0004.

[0024] 3. Experimental method

[0025] SD rats were randomly divided into 6 groups, respectively blank control group, model control group, heparin (500IU / kg) group, compound Xueshuantong capsule low dose (380mg / kg) group, compound Xueshuantong capsule medium dose (760mg / kg) group, kg) group, compound Xueshuantong capsule high dose (1520mg / kg) group, 10 rats in each group. After the experimental animals were adapted to the feeding environment for one week, they were administered orally once a day, and the dosage volume was 10 mL / kg. The blank control group...

Embodiment 2

[0033] Example 2: Determination of expression of NF-κB inflammatory signaling pathway

[0034] 1. Experimental materials:

[0035] RIPA protein extraction kit, BCA protein assay kit, rabbit anti-NF-κB p65 antibody, IκB-α antibody and β-actin antibody, HRP-labeled goat anti-mouse antibody (IgG) were purchased from Biyuntian Biotechnology Research Institute; photosensitive film : Kodak FF057; other common western reagent consumables such as SDS-PAGE loading buffer, electrophoresis solution, nitrocellulose membrane, transfer solution, Beyo-ECL luminescent solution were purchased from Beyontian Biotechnology Research Institute.

[0036] 2. Experimental method:

[0037] The rat liver tissue of Example 1 was taken to weigh and homogenate, and the total protein of each group was determined by BCA method. Adjust the total protein of each group to a uniform level, add 5×SDS-PAGE loading buffer, cook in boiling water for 10 minutes, and store the samples at -20°C. Prepare SDS-PAGE ge...

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PUM

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Abstract

The invention discloses the application of a compound thrombus links agent in preparing a medicine for inhibiting NF-kB pathway activation, a medicine for inhibiting raising of inflammatory factors ofTNF-alpha, IL-6, IL-8 or MCP-1 and other anti-inflammatory medicines. Proved by a pharmacological experiment in vivo of an inflammatory thrombus rat model induced by LPS, the compound thrombus linksagent can obviously inhibit the raising of the inflammatory factors of TNF-alpha, IL-6, IL-8 and MCP-1. Further research finds that the mechanism inhibits the degradation of IkB-alpha and the nucleation of p65, thereby inhibiting the activation of the NF-kB pathway.

Description

technical field [0001] The invention belongs to the field of Chinese patent medicines, and in particular relates to the anti-inflammatory application of compound Xueshuantong preparation. Background technique [0002] Nuclear transcription factor-κB (nuclear transcription factor-κB, NF-κB) is a regulatory transcription factor that enters the nucleus and can initiate the expression of various inflammatory genes. NF-κB is mostly a heterodimer of p65 and p50, usually combined with the inhibitory protein IκB-α to form an inactive form in the cytoplasm, when cells are stimulated by bacteria, oxidants, toxins, and pro-inflammatory cytokines , IκB-α undergoes rapid and massive phosphorylation and is hydrolyzed by non-specific proteases, thereby exposing the nuclear localization signal of Rel A protein, and the activated NF-κB migrates to the nucleus and binds to the promoter sequence of the induced gene. Corresponding κB sites, thereby initiating the transcription and expression o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/808A61P29/00
Inventor 苏薇薇刘宏吴灏生书晶
Owner SUN YAT SEN UNIV