Haworthia truncatapropagating method by test tube micro-cuttage

A technology of jade fan brocade and micro-cutting, which is applied in the direction of vegetative propagation, horticultural methods, botanical equipment and methods, etc., can solve the problems of high price, low survival rate of jade fan brocade leaf cuttings, high ornamental value, etc., and achieve stability , high economic value and practical effect

Active Publication Date: 2019-05-28
ZHANGZHOU INST OF AGRI SCI
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Problems solved by technology

In the greenhouse, the leaf cuttings of Yushanjin are used. Because the leaves of Yushanjin are thicker, have higher water content, and are affected by the environment, the leaves are easy to rot a

Method used

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  • Haworthia truncatapropagating method by test tube micro-cuttage
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  • Haworthia truncatapropagating method by test tube micro-cuttage

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Embodiment Construction

[0029] (1) Selection and disinfection of materials Take the finished seedlings of Jade Shanjin as the material, put them in the greenhouse to control the water for 1 week, then pull them out, cut off the roots, rinse them, and then place them on the ultra-clean workbench with 75% alcohol Soak for 30s, then sterilize with 0.1% mercuric acid solution containing 1-2 drops of Tween-80 for 10 minutes, rinse with sterile water for 3 to 5 times, and filter the residual water with sterile filter paper;

[0030] (2) Under aseptic conditions, remove the leaves of the sterilized jade fan without brocade, and keep the integrity of the growth point of the base of the leaves with brocade during the grafting process. In order to increase the bud induction rate of Yufan leaves, the basic medium MS was improved, and the improvement method was as follows: reduce the amount of ammonium nitrate in MS, and increase the amount of potassium dihydrogen phosphate , added calcium nitrate. The improved...

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Abstract

The invention discloses a haworthia truncata propagating method by test tube micro-cuttage. The method comprises the following steps that (1), a material is selected and disinfected; (2), under aseptic conditions, leaves without stripes of the disinfected haworthia truncata are removed, leaves with stripes are connected to a culture medium for inducing leaf blade bases to grow; (3), the induced haworthia truncata plantlets are connected to a root medium, the haworthia truncataplantlets are cultivated until the haworthia truncata plantlets grow to root seedlings with the diameter of 2.8-3.2cm;and (4), the haworthia truncataroot seedlings with good root systems and complete blades are subjected to adaptive seedling refining for 18-22 days in a seedling hardening shed, then the hardened rootseedlings are taken out and disinfected,the disinfected haworthia truncata seedlings are placed on a cool and ventilated place to be air-dried until the blades of the haworthia truncata group cultureseedlings wrinkle, and transplant can be conducted. The haworthia truncata can be successfully propagated.

Description

technical field [0001] The invention relates to a method for propagating jade fan brocade by micro-cutting in test tubes. Background technique [0002] Jade fan (Haworthia truncata), also known as truncated twelve volumes, is a small succulent plant of the genus Twelve Volumes in the family Liliaceae, native to southern Africa. Its plants are dignified, beautiful in shape, rich in variety variation, and rich in window patterns. It is very suitable for home maintenance and is a precious species that succulent lovers like to collect. [0003] The traditional propagation methods of Twelve Volumes mainly include cuttings, divisions and sowing. The survival rate of cuttings is extremely low, which is suitable for small-scale propagation of plants by plant lovers, but not suitable for commercial large-scale propagation. Branching is a commonly used traditional propagation method of the genus Twelve, but the annual reproduction amount is only 3-5, there are fewer rare varieties,...

Claims

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Application Information

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IPC IPC(8): A01G22/60A01G2/10A01H4/00
Inventor 陈汉鑫余德亿万学锋姚锦爱
Owner ZHANGZHOU INST OF AGRI SCI
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