Nanofiber-based composite hemodialysis membrane and preparation method thereof

Abstract

The invention relates to a nanofiber-based composite hemodialysis membrane and a preparation method thereof. The preparation method includes the steps that a high-molecular polymer solution is subjected to electrostatic spinning, a nanofiber non-woven fabricis obtained, and subjected to cold pressing, and then a nanofiber porous supporting layer is obtained; after silk is subjected to degumming, dissolving, dialyzing, vulcanizing and self-assembling, a vulcanized silk fibroin protein nanowire precursor is obtained; a prepared vulcanized silk fibroin protein nanowire and polyvinyl alcohol are added into water; and then glutaric dialdehyde is added to be crosslinked, the mixture is coated in the nanofiber porous supporting layer, and the nanofiber-based composite hemodialysis membrane can beobtained after sealing at the room temperature. According to the preparation method of the nanofiber-based composite hemodialysis membrane, silk materials with the characteristics of low cost and wide sources are used for preparing the vulcanized silk fibroin protein nanowire, the process is simple, and implementation is easy; and the obtained nanofiber-based composite hemodialysis membrane has good water permeability, the excellent dialysis efficiency and anticoagulant activity.

Description

technical field

[0001] The invention belongs to the field of biological materials and their preparation, in particular to a nanofiber-based composite hemodialysis membrane and a preparation method thereof. Background technique

[0002] With the aging of the population in the world, the number of patients with chronic renal failure and acute uremia has increased sharply. About 4 million people in my country suffer from acute kidney disease every year. How to effectively solve renal diseases such as renal failure has become a research hotspot for researchers around the world. Hemodialysis, as one of the main ways to effectively treat patients with acute and chronic renal failure, has been widely used in recent years. The hemodialysis membrane plays a decisive role in the hemodialysis system, and the surface structure of the hemodialysis membrane directly affects the efficiency of hemodialysis.

[0003] Because the hemodialysis membrane is in direct contact with the blood, it ...

Examples

Embodiment 1

[0045] This embodiment provides a nanofiber-based composite hemodialysis membrane, the nanofiber-based composite hemodialysis membrane has a nanofiber composite membrane structure, including an outer layer and an inner layer, and the outer layer of the nanofiber-based composite hemodialysis membrane is Anticoagulant sulfided silk fibroin nanowires modified polyvinyl alcohol hydrogel skin layer, the inner layer is a nanofiber porous support layer prepared by electrospinning.

[0046] The thickness of the polyvinyl alcohol hydrogel cortex modified by the anticoagulant sulfurized silk fibroin nanowires is about 0.6 μm, the average diameter of the nanofibers in the nanofiber porous support layer is 300 nm, the deposition thickness is 40 μm, and the porosity 75%.

[0047] Concrete preparation steps are as follows:

[0048] Step 1: Polyacrylonitrile (PAN, M w =120000g / mol) 16g was dissolved in 184g of N,N-dimethylformamide (DMF) solvent, stirred at 40°C for 6h to obtain a transpar...

Embodiment 2

[0054] This embodiment provides a nanofiber-based composite hemodialysis membrane, the nanofiber-based composite hemodialysis membrane has a nanofiber composite membrane structure, including an outer layer and an inner layer, and the outer layer of the nanofiber-based composite hemodialysis membrane is Anticoagulant sulfided silk fibroin nanowires modified polyvinyl alcohol hydrogel skin layer, the inner layer is a nanofiber porous support layer prepared by electrospinning.

[0055] The thickness of the polyvinyl alcohol hydrogel cortex modified by the anticoagulant sulfurized silk fibroin nanowires is about 0.6 μm, the average diameter of the nanofibers in the nanofiber porous support layer is 300 nm, the deposition thickness is 40 μm, and the porosity 75%.

[0056] Concrete preparation steps are as follows:

[0057] Step 1 is the same as embodiment 1;

[0058] Step 2 is the same as embodiment 1;

[0059] Step 3 is the same as embodiment 1;

[0060] Step 4: Freeze-dry the...

Embodiment 3

[0063] like figure 2 As shown, this embodiment provides a nanofiber-based composite hemodialysis membrane, the nanofiber-based composite hemodialysis membrane has a nanofiber composite membrane structure, including an outer layer and an inner layer, and the nanofiber-based composite hemodialysis membrane The outer layer is a polyvinyl alcohol hydrogel skin layer modified by anticoagulation sulfurized silk fibroin nanowires, and the inner layer is a nanofiber porous support layer prepared by electrospinning.

[0064] The thickness of the polyvinyl alcohol hydrogel cortex modified by the anticoagulant sulfided silk fibroin nanowires is about 0.6 μm, such as figure 1 As shown, the nanofibers of the nanofiber porous support layer have an average diameter of 300 nm, a deposition thickness of 40 μm, and a porosity of 75%.

[0065] Concrete preparation steps are as follows:

[0066] Step 1 is the same as embodiment 1;

[0067] Step 2 is the same as embodiment 1;

[0068] Step 3 ...

technical field

[0001] The invention belongs to the field of biological materials and their preparation, in particular to a nanofiber-based composite hemodialysis membrane and a preparation method thereof. Background technique

[0002] With the aging of the population in the world, the number of patients with chronic renal failure and acute uremia has increased sharply. About 4 million people in my country suffer from acute kidney disease every year. How to effectively solve renal diseases such as renal failure has become a research hotspot for researchers around the world. Hemodialysis, as one of the main ways to effectively treat patients with acute and chronic renal failure, has been widely used in recent years. The hemodialysis membrane plays a decisive role in the hemodialysis system, and the surface structure of the hemodialysis membrane directly affects the efficiency of hemodialysis.

[0003] Because the hemodialysis membrane is in direct contact with the blood, it ...

Examples

Embodiment 1

[0045] This embodiment provides a nanofiber-based composite hemodialysis membrane, the nanofiber-based composite hemodialysis membrane has a nanofiber composite membrane structure, including an outer layer and an inner layer, and the outer layer of the nanofiber-based composite hemodialysis membrane is Anticoagulant sulfided silk fibroin nanowires modified polyvinyl alcohol hydrogel skin layer, the inner layer is a nanofiber porous support layer prepared by electrospinning.

[0046] The thickness of the polyvinyl alcohol hydrogel cortex modified by the anticoagulant sulfurized silk fibroin nanowires is about 0.6 μm, the average diameter of the nanofibers in the nanofiber porous support layer is 300 nm, the deposition thickness is 40 μm, and the porosity 75%.

[0047] Concrete preparation steps are as follows:

[0048] Step 1: Polyacrylonitrile (PAN, M w =120000g / mol) 16g was dissolved in 184g of N,N-dimethylformamide (DMF) solvent, stirred at 40°C for 6h to obtain a transpar...

Embodiment 2

[0054] This embodiment provides a nanofiber-based composite hemodialysis membrane, the nanofiber-based composite hemodialysis membrane has a nanofiber composite membrane structure, including an outer layer and an inner layer, and the outer layer of the nanofiber-based composite hemodialysis membrane is Anticoagulant sulfided silk fibroin nanowires modified polyvinyl alcohol hydrogel skin layer, the inner layer is a nanofiber porous support layer prepared by electrospinning.

[0055] The thickness of the polyvinyl alcohol hydrogel cortex modified by the anticoagulant sulfurized silk fibroin nanowires is about 0.6 μm, the average diameter of the nanofibers in the nanofiber porous support layer is 300 nm, the deposition thickness is 40 μm, and the porosity 75%.

[0056] Concrete preparation steps are as follows:

[0057] Step 1 is the same as embodiment 1;

[0058] Step 2 is the same as embodiment 1;

[0059] Step 3 is the same as embodiment 1;

[0060] Step 4: Freeze-dry the...

Embodiment 3

[0063] like figure 2 As shown, this embodiment provides a nanofiber-based composite hemodialysis membrane, the nanofiber-based composite hemodialysis membrane has a nanofiber composite membrane structure, including an outer layer and an inner layer, and the nanofiber-based composite hemodialysis membrane The outer layer is a polyvinyl alcohol hydrogel skin layer modified by anticoagulation sulfurized silk fibroin nanowires, and the inner layer is a nanofiber porous support layer prepared by electrospinning.

[0064] The thickness of the polyvinyl alcohol hydrogel cortex modified by the anticoagulant sulfided silk fibroin nanowires is about 0.6 μm, such as figure 1 As shown, the nanofibers of the nanofiber porous support layer have an average diameter of 300 nm, a deposition thickness of 40 μm, and a porosity of 75%.

[0065] Concrete preparation steps are as follows:

[0066] Step 1 is the same as embodiment 1;

[0067] Step 2 is the same as embodiment 1;

[0068] Step 3 ...

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