Preparation and application of photoelectricity and temperature dual-signal response sialic acid bio-sensor based on polymer dots

A biosensor, polymer dot technology, applied in the field of biosensors, can solve the problems of limited application and development, tedious preliminary preparation, expensive detection instruments, etc., to achieve excellent photothermal performance, widen detection range, and good reproducibility.

Inactive Publication Date: 2019-05-28
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these detection methods require expensive detection instruments, complicated operation procedures and tedious preparations, which greatly limit their application and development.

Method used

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  • Preparation and application of photoelectricity and temperature dual-signal response sialic acid bio-sensor based on polymer dots
  • Preparation and application of photoelectricity and temperature dual-signal response sialic acid bio-sensor based on polymer dots
  • Preparation and application of photoelectricity and temperature dual-signal response sialic acid bio-sensor based on polymer dots

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Experimental program
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Effect test

Embodiment 1

[0033] 1. A preparation method and application of a biosensor based on photoelectric and temperature dual-signal responses of polymer dots, characterized in that, comprising the following steps:

[0034] (1) Mechanically polish the glassy carbon electrode (GCE) with 50 nanometers and 30 nanometers of alumina powder on the wet suede until the surface of the electrode is bright and clean, and rinse the residual alumina powder on the surface of the electrode with deionized water , and finally rinse thoroughly with absolute ethanol and secondary deionized water.

[0035] (2) Add dropwise 3 μL of fullerene (C 60 ) solution (the solvent is dimethylformamide) on the surface of a polished glassy carbon electrode, put it in an infrared light box for drying, take it out, and cool it at room temperature to obtain C 60 Modified glassy carbon electrode;

[0036] (3) Drop 3 μL of gold nanometer (Au) solution with a concentration of 3 mg / mL on the surface of the electrode prepared in step ...

Embodiment 2

[0044] The sector-shaped rutile-type titanium dioxide (R-TiO2) used in above-mentioned embodiment 1 2 ) was prepared by the following method: 1 g sodium dodecylbenzene sulfonate (SDBS) was dissolved in 50 mL 2.2 mol / L HNO 3 Solution, shake and mix on a vortex mixer for tens of minutes, then add 1 mL of isopropyl titanate (TIP), then shake the above mixture for 24 hours at 48 °C, and react with deionized water and ethanol The resulting mixture was washed several times, centrifuged, dried at 60°C, and then calcined at 400°C for 1 h to remove residual organic components in the product to obtain the final product. The obtained product was evenly dispersed in 1 mL of deionized water (use methods such as ultrasonic mixing to make it uniformly dispersed), and the obtained R-TiO 2 The solution was placed in a 4°C refrigerator and stored for future use.

Embodiment 3

[0046] The preparation method of the tetraphenol-doped polymer dot (TPP-pdot) used in the above Example 1 is as follows: polymer (PFBT), functional polymer co-maleic anhydride (PSMA) and sensitizer tetraphenol ( TPP) were dissolved in tetrahydrofuran (THF), and dispersed uniformly by ultrasonication. Mix the above three solutions. In the mixture, the concentration of PFBT is 200 µg / mL, the concentration of PSMA is 40 µg / mL, and the concentration of TPP is 10 µg / mL. Then the obtained mixed solution was ultrasonicated for 20 min to form a uniformly dispersed solution. Under ultrasonic conditions in a water bath, 4 mL of the above mixture was quickly added to 20 mL of deionized water. The THF solvent molecules in the solution were purged with nitrogen, and finally the mixture was rotated and evaporated at 55° C. to fully remove residual THF molecules to obtain a yellow transparent liquid.

[0047] The poly[(9,9-dioctylfluorene-2,7-diyl)-alternate-(2,1,3-benzothiazole)](PFBT), p...

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Abstract

The invention discloses preparation and application of a photoelectricity and temperature dual-signal response sialic acid bio-sensor based on polymer dots. According to the sensor, two nano-materialsof fullerene (C60) and a Au cone serve as a substrate of the sensor, mercaptophenylboronic acid (MPBA) molecules are fixed through the bonding effect of Au-S bonds, and a large number of sialic acid(SA) molecules are captured. Antibodies marked by intertetraphenol doped polymer dot (TPP-pdot) functionalized sector rutile type titanium dioxide (R-Tio2) serve as probe molecules, excellent photocurrent response and good temperature response are achieved, and a dual-signal reading pattern is obtained. Based on the advantages, the dual-signal response bio-sensor is established, high-sensitivity detection of sialic acid is achieved, compared with a traditional single-signal reading sensor, the sensor is higher in accuracy and better in stability, and the situation that the detection result isfalsely positive or negative is avoided to a large degree.

Description

technical field [0001] The invention belongs to the fields of preparation and application of new nanometer materials and bioimmune sensing and detection, and specifically designs a biosensor based on photoelectricity and temperature dual-signal response of polymer dots for highly sensitive detection of sialic acid molecules. Background technique [0002] Sialic acid molecule (sialic acid, SA) is an acetylated derivative widely present in various tissue cells of the human body. It is related to various biological functions of the organism and participates in various physiological processes of the organism. Studies have shown that the increase of SA content in human serum is closely related to the development of tumors, so the development of a sensitive and practical detection method to realize the detection of SA in serum is useful for early screening and detection of related diseases Significance. So far, a series of methods have been used for the detection of SA content, s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327G01N33/531
Inventor 戴宏陈妍洁王佳妮高利红衣欢宋建榕郑祥钦韩晴
Owner FUJIAN NORMAL UNIV
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