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A Hexabromocyclododecane Degrading Strain and Its Application in Environmental Remediation

A technology of hexabromocyclododecane and bacterial strain, which is applied in the field of environmental biology and achieves the effects of mild reaction conditions, low cost and energy saving

Active Publication Date: 2022-04-12
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above-mentioned defects of the prior art, the technical problem to be solved by the present invention is how to reduce the cost of environmental pollution treatment while improving the degradation efficiency of hexabromocyclododecane

Method used

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  • A Hexabromocyclododecane Degrading Strain and Its Application in Environmental Remediation
  • A Hexabromocyclododecane Degrading Strain and Its Application in Environmental Remediation
  • A Hexabromocyclododecane Degrading Strain and Its Application in Environmental Remediation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Isolation and identification of Pseudomonas aeruginosa HS9 and its degradation characteristics of HBCDs

[0048] 1. Screening and isolation of strains

[0049] 1) Take a sample

[0050] Soil sample collection location: Laogang Industrial Zone, Shanghai.

[0051] 2) Screening and isolation of strains

[0052] Dissolve a total of 10 g of soil samples collected from different depths in 50 mL of distilled water, shake on a vortex shaker for 5 minutes, take 5 mL of soil solution, add it to 50 mL of inorganic salt liquid medium, and culture at a temperature of 30 °C and a speed of 200 rpm. Minutes for 10 days. After 3 to 4 passages, the culture solution was diluted and spread on the inorganic salt solid slant medium added with 1.0 mg / L HBCDs, and cultured at 30° C. for 5 to 10 days. Pick a single colony into a fresh inorganic salt liquid medium, select the fastest-growing strain for streak isolation, and repeat several times until a purified single bacterium is ...

Embodiment 2

[0067] Example 2 Analysis of intermediate metabolites of Pseudomonas aeruginosa HS9 degrading HBCDs

[0068] 1. Culture of Pseudomonas aeruginosa HS9

[0069] Inoculate 15 mL of bacterial suspension into 1 L of MSM liquid medium containing HBCDs (1.0 mg / L), culture at 30° C., and rotate at 200 rpm. When the growth of the strain entered the late plateau stage, the cells were collected for resting cell reaction.

[0070] Add HBCDs (1.0mg / L) as a substrate to stop the cell reaction. During the reaction for 0 to 24 hours, take 10 mL of the culture, centrifuge at 8,000 rpm for 10 minutes at 4°C, and collect the supernatant. Extraction for subsequent gas chromatography-mass spectrometry (GC-MS) and HPLC-MS analysis.

[0071] 2. Sample preparation process and chromatographic conditions

[0072] 1) Sample preparation

[0073] Add 10 μL HCl to 1 mL sample to terminate the reaction, to reduce the low extraction effect caused by adsorption. After shaking with a vortex shaker for 30 ...

Embodiment 3

[0081] Example 3 Simulation application of Pseudomonas aeruginosa HS9 in environmental restoration

[0082] Because the actual pollution environment is usually relatively harsh, therefore, in order to simulate the application of Pseudomonas aeruginosa HS9 to remove HBCDs in wastewater, the adaptation range of the bacterial strain to the culture temperature and the initial pH of the culture solution has been tested (initial HBCDs concentration 1.7mg / L, 200 rpm shaking culture). Such as image 3 As shown, the strains can degrade HBCDs rapidly when the culture temperature is 25°C-37°C. Such as Figure 4 As shown, the strains can degrade HBCDs rapidly in the inorganic salt liquid medium with a pH value of 6.0-8.0. Among them, when the pH value was 7.0, the degradation rate of HBCDs was the fastest; when the pH value was 6.0 or 8.0, the strain's degradation rate of HBCDs decreased. Under the optimum conditions of 30°C and pH 7.0, such as Image 6 In the simulation application ...

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Abstract

The invention discloses a hexabromocyclododecane-degrading bacterium and its application in environmental restoration. The bacterium is Pseudomonas aeruginosa HS9, which was deposited in the China Center for Type Culture Collection on February 18, 2019, with the preservation number CCTCC NO.M2019094. The Pseudomonas aeruginosa (Pseudomonas aeruginosa) HS9 of the present invention has high-efficiency degradation rate and degradation speed for hexabromocyclododecane, and as an environmental biocatalyst, it not only has low cost and mild reaction conditions, but also saves energy.

Description

technical field [0001] The invention relates to the field of environmental biotechnology, in particular to a hexabromocyclododecane-degrading bacterial strain and its application in environmental restoration. Background technique [0002] Brominated flame retardants (BFRs) are currently one of the organic flame retardants with the largest output and the highest flame retardant efficiency in the world, and are widely used in various fields of national production and people's daily life. With the mass production and widespread use of brominated flame retardants, the environmental problems caused by brominated flame retardants have become increasingly prominent. Among them, hexabromocyclododecane (1,2,5,6,9,10-hexabromocyclododecanes, HBCDs), as one of the main representatives of brominated flame retardants, is mainly used in building materials expanded polystyrene board (EPS) And extruded polystyrene board (XPS), textiles and high impact polystyrene (HIPS). Due to the persis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/385C02F101/36
Inventor 唐鸿志黄玲许平王伟伟陶飞
Owner SHANGHAI JIAOTONG UNIV
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