A kind of traditional Chinese medicine mouthwash and its preparation method and application
A technology of mouthwash and traditional Chinese medicine, which is applied in the field of preparation of oral health preparations, can solve the problems of not suitable for oral care of patients, low acceptance, turbid color, etc., and achieve the effects of slightly sweet taste, less infection, and easy preparation method
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Embodiment 1
[0020] Embodiment 1, prepare 100g Chinese medicine mouthwash
[0021] 1. Recipe
[0022]
[0023] Vitamin B complex is a compound preparation (commercially available), containing vitamin B1, vitamin B2, vitamin B6, niacinamide, and calcium pantothenate.
[0024] The sweetener is xylitol, the preservative is sorbic acid, and the active ingredient stabilizer is Tween 20.
[0025] 2, a preparation method of Chinese medicine mouthwash, the steps are as follows:
[0026] (1) Preparation of each selected single-flavor traditional Chinese medicine extract: place the selected traditional Chinese medicines (mint leaves, Radix Radix, Salvia miltiorrhiza, Scutellaria baicalensis) in a container, add 3 times the weight of water, soak at 4°C for 4h; boil again 1h, let stand, filter, and take the filtrate to obtain the extract of each single Chinese medicine;
[0027] (2) Mix the traditional Chinese medicine extract obtained in (1) according to the formula amount, add the ultrapure wa...
Embodiment 2
[0028] Embodiment 2, prepare 100g Chinese medicine mouthwash
[0029] 1. Recipe
[0030]
[0031] The vitamin B complex is a compound preparation (commercially available), containing vitamin B1, vitamin B2, vitamin B6, niacinamide, and calcium pantothenate.
[0032] The sweetener is aspartame (C 14 H 18 N 2 O 5 , Mr=294.30), the preservative is sodium benzoate, and the active ingredient stabilizer is Tween 20.
[0033] 2. The preparation method of a traditional Chinese medicine mouthwash is the same as in Example 1.
Embodiment 3
[0034] Embodiment 3. Determination of the antibacterial effect of mouthwash
[0035] (1) Preparation of agar plates
[0036] The mouthwashes prepared in Example 1 and Example 2 were added to the agar medium that was melted and cooled to about 60°C according to the volume ratio of 3:7, and the same amount of sterilized water was used as a negative control under the same conditions. The composition of agar medium: tryptone 1.0g, yeast extract 0.5g, sodium chloride 1.0g, agar 1.5g, dilute to 100mL with deionized water, sterilize under high pressure for 20 minutes, pour the plate to become an agar plate.
[0037] (2) Inoculate bacteria
[0038] E. coli (ATC8099) was grown to OD at 37°C, 180rpm 600 =0.6-0.8, obtain Escherichia coli bacterial liquid. After the agar plate prepared in step (1) was completely condensed, 0.05 mL of Escherichia coli bacterial liquid was taken and evenly spread on the above plate, and cultured at 37°C overnight.
[0039] The results showed that a larg...
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